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Information on EC 2.1.1.240 - trans-resveratrol di-O-methyltransferase

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.240 trans-resveratrol di-O-methyltransferase
IUBMB Comments
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
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This record set is specific for:
UNIPROT: A8QW53
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
resveratrol o-methyltransferase, sbromt3syn, vrromt, sbomt3, vrromtsyn, vrromtsyn protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-pentadecatrienyl resorcinol O-methyltransferase
UniProt
resveratrol O-methyltransferase
-
SbROMT3syn protein
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:trans-resveratrol 3,5-O-dimethyltransferase
The enzyme catalyses the biosynthesis of pterostilbene from resveratrol.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene
S-adenosyl-L-homocysteine + pterostilbene
show the reaction diagram
S-adenosyl-L-methionine + trans-resveratrol
S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3-methoxy-4',5-dihydroxy-trans-stilbene
S-adenosyl-L-homocysteine + pterostilbene
show the reaction diagram
i.e. pinostilbene
-
-
?
S-adenosyl-L-methionine + trans-resveratrol
S-adenosyl-L-homocysteine + 3-methoxy-4',5-dihydroxy-trans-stilbene
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
OMT3_SORBI
374
0
40884
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42500
x * 42500, recombinant enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 42500, recombinant enzyme, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3)-RIPL by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene sbOMT3, recombinant expression of codon-optimized resveratrol O-methyltransferase genes sbOMT1 and sbOMT3 in Escherichia coli strain C41 (DE3), development of an artificial biosynthetic pathway that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources in Escherichia coli
gene SbROMT3syn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha
recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, coexpression with His-tagged cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor and Strep-tagged stilbene synthase from Rheum palmatum
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
large-scale production of plant metabolites via microbial approaches is a promising alternative to chemical synthesis and extraction from plant sources Development of an Escherichia coli system containing an artificial biosynthetic pathway, involving the enzyme, that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources
synthesis
the enzyme from Sorghum bicolor, SbROMT3syn, can be used as an enzyme to produce pinostilbene by methylating resveratrol in microorganisms
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kang, S.Y.; Lee, J.K.; Choi, O.; Kim, C.Y.; Jang, J.H.; Hwang, B.Y.; Hong, Y.S.
Biosynthesis of methylated resveratrol analogs through the construction of an artificial biosynthetic pathway in E. coli
BMC Biotechnol.
14
67
2014
Sorghum bicolor (A8QW53)
Manually annotated by BRENDA team
Jeong, Y.J.; An, C.H.; Woo, S.G.; Jeong, H.J.; Kim, Y.M.; Park, S.J.; Yoon, B.D.; Kim, C.Y.
Production of pinostilbene compounds by the expression of resveratrol O-methyltransferase genes in Escherichia coli
Enzyme Microb. Technol.
54
8-14
2014
Sorghum bicolor (A8QW53), Vitis riparia (K7XQ68)
Manually annotated by BRENDA team
Jeong, Y.J.; Woo, S.G.; An, C.H.; Jeong, H.J.; Hong, Y.S.; Kim, Y.M.; Ryu, Y.B.; Rho, M.C.; Lee, W.S.; Kim, C.Y.
Metabolic engineering for resveratrol derivative biosynthesis in Escherichia coli
Mol. Cells
38
318-326
2015
Sorghum bicolor (A8QW53), Vitis riparia (K7XQ68)
Manually annotated by BRENDA team