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Information on EC 2.1.1.238 - mycinamicin VI 2''-O-methyltransferase and Organism(s) Micromonospora griseorubida and UniProt Accession Q83WF2

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.238 mycinamicin VI 2''-O-methyltransferase
IUBMB Comments
The enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics. Requires Mg2+ for optimal activity.
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This record set is specific for:
Micromonospora griseorubida
UNIPROT: Q83WF2
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The taxonomic range for the selected organisms is: Micromonospora griseorubida
The enzyme appears in selected viruses and cellular organisms
Synonyms
MycE, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:mycinamicin VI 2''-O-methyltransferase
The enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics. Requires Mg2+ for optimal activity.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + mycinamicin VI
S-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + mycinamicin VI
S-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram
-
the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation is about 50% of the activation obtained by Mg2+
Fe2+
-
activation is about 70% of the activation obtained by Mg2+
Mg2+
-
optimal activity in presence of 10 mM MgCl2
Mn2+
-
activation is about 70% of the activation obtained by Mg2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
significantly lowers the activity of MycE even in the presence of 10 mM Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0264
mycinamicin VI
-
pH 7.4, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.083
mycinamicin VI
-
pH 7.4, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.14
mycinamicin VI
-
pH 7.4, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
construction of a MycE disrupion mutant. The disruption mutant mainly produces mycinamicin VI
physiological function
-
the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics. Mycinamicins represent a family of macrolide antibiotics with more than twenty members produced by the rare actinomycete Micromonospora griseorubida
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MYCE_MICGR
399
0
44809
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44800
-
44800, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
44800, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant N-terminal His6-tagged protein, purified by one-step Ni-NTA agarose chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
MycE is cloned into pET28b for overexpression in Escherichia coli BL21 (DE3)
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
the antibacterial activities of some mycinamicin products against Staphylococcus aureus are higher than those of clinical macrolide antibiotics erythromycin and leucomycin
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li, S.; Anzai, Y.; Kinoshita, K.; Kato, F.; Sherman, D.H.
Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics
Chembiochem
10
1297-1301
2009
Micromonospora griseorubida
Manually annotated by BRENDA team
Anzai, Y.; Saito, N.; Tanaka, M.; Kinoshita, K.; Koyama, Y.; Kato, F.
Organization of the biosynthetic gene cluster for the polyketide macrolide mycinamicin in Micromonospora griseorubida
FEMS Microbiol. Lett.
218
135-141
2003
Micromonospora griseorubida (Q83WF2), Micromonospora griseorubida A11725 (Q83WF2)
Manually annotated by BRENDA team
Tsukada, S.; Anzai, Y.; Li, S.; Kinoshita, K.; Sherman, D.H.; Kato, F.
Gene targeting for O-methyltransferase genes, mycE and mycF, on the chromosome of Micromonospora griseorubida producing mycinamicin with a disruption cassette containing the bacteriophage phi C31 attB attachment site
FEMS Microbiol. Lett.
304
148-156
2010
Micromonospora griseorubida
Manually annotated by BRENDA team