Substrates: the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Products: -
?
S-adenosyl-L-methionine + adenosine4 in tRNAHis
S-adenosyl-L-homocysteine + 2'-O-methyladenosine4 in tRNAHis
Substrates: the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Products: -
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
Substrates: the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Products: -
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
Substrates: Trm13p belongs to the RFM superfamily of MTases despite the absence of a significant sequence similarity to previously characterized members of this superfamily. The model reveals residues potentially responsible for cofactor binding, tRNA binding, and catalysis of the 2'-O-methylation reaction. The model suggests a presence of one to three disulfide bonds and/or metal ion binding site next to the substrate binding pocket Products: -
Substrates: Trm13p belongs to the RFM superfamily of MTases despite the absence of a significant sequence similarity to previously characterized members of this superfamily. The model reveals residues potentially responsible for cofactor binding, tRNA binding, and catalysis of the 2'-O-methylation reaction. The model suggests a presence of one to three disulfide bonds and/or metal ion binding site next to the substrate binding pocket Products: -
Substrates: the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Products: -
?
S-adenosyl-L-methionine + cytidine4 in tRNAGly(GCC)
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAGly(GCC)
Substrates: the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Products: -
?
S-adenosyl-L-methionine + cytidine4 in tRNAPro
S-adenosyl-L-homocysteine + 2'-O-methylcytidine4 in tRNAPro
Substrates: the enzyme is necessary and sufficient for 2'-O-methylation at position 4 of yeast tRNA. 2'-O-methylation in the acceptor stem of tRNA Products: -
Trm13p, the tRNA:Xm4 modification enzyme from Saccharomyces cerevisiae is a member of the Rossmann-fold MTase superfamily: prediction of structure and active site
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