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Information on EC 2.1.1.22 - carnosine N-methyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P53934

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.22 carnosine N-methyltransferase
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Saccharomyces cerevisiae
UNIPROT: P53934 not found.
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Synonyms
carnosine n-methyltransferase, ynl092w, c9orf41, upf0586 protein c9orf41 homolog, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:carnosine N-methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37256-93-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structure in complex with SAH. At the cofactor-binding site, SAH forms an extensive interaction network with the enzyme. Two disulfide bridges might be functionally important to the enzyme
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C123A
mutation in disulfide bridge, displays strongly enhanced activity compared to that of wild-type
C238A
mutation in disulfide bridge, significantly reduces the activity
D278A/T279A
mutant barely displays any affinity to SAM
E167A
mutant barely displays any affinity to SAM
H309A
about 40% of wild-type activity
L307F
activity similar to wild-type
N274A
substantial reduction in expression level. Mutant barely displays any affinity to SAM
Q108A
mutant barely displays any affinity to SAM
R111A
mutant barely displays any affinity to SAM
S104A
activity similar to wild-type
S234A
mutant barely displays any affinity to SAM
Y286A
about 10fold lower affinity to SAM than wild-type
Y371A
substantial reduction in expression level, mutant barely displays any affinity to SAM
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Liu, X.; Wu, J.; Sun, Y.; Xie, W.
Substrate recognition mechanism of the putative yeast carnosine N-methyltransferase
ACS Chem. Biol.
12
2164-2171
2017
Saccharomyces cerevisiae (P53934), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (P53934)
Manually annotated by BRENDA team