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Information on EC 2.1.1.217 - tRNA (adenine22-N1)-methyltransferase and Organism(s) Bacillus subtilis and UniProt Accession P54471

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     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.217 tRNA (adenine22-N1)-methyltransferase
IUBMB Comments
The enzyme specifically methylates adenine22 in tRNA.
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This record set is specific for:
Bacillus subtilis
UNIPROT: P54471
The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
Sp1610, TrmK, tRNA: m1A22 methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TrmK
293156
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tRNA: m1A22 methyltransferase
293156
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (adenine22-N1)-methyltransferase
The enzyme specifically methylates adenine22 in tRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine22 in Bacillus subtilis tRNA(Ser)
S-adenosyl-L-homocysteine + N1-methyladenine22 in Bacillus subtilis tRNA(Ser)
show the reaction diagram
crude Escherichia coli tRNA is used, since it does not contain m1A22
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-
?
S-adenosyl-L-methionine + adenine22 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine22 in tRNA
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA
show the reaction diagram
P54471
a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules
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-
?
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roovers, M.; Kaminska, K.H.; Tkaczuk, K.L.; Gigot, D.; Droogmans, L.; Bujnicki, J.M.
The YqfN protein of Bacillus subtilis is the tRNA: m1A22 methyltransferase (TrmK)
Nucleic Acids Res.
36
3252-3262
2008
Bacillus subtilis, Bacillus subtilis (P54471)
Manually annotated by BRENDA team
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