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Information on EC 2.1.1.212 - 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase and Organism(s) Lotus japonicus and UniProt Accession Q84KK4

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IUBMB Comments
Specifically methylates 2,4',7-trihydroxyisoflavanone on the 4'-position. No activity with isoflavones . The enzyme is involved in formononetin biosynthesis in legumes . The protein from pea (Pisum sativum) also methylates (+)-6a-hydroxymaackiain at the 3-position (cf. EC 2.1.1.270, (+)-6a-hydroxymaackiain 3-O-methyltransferase) .
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Lotus japonicus
UNIPROT: Q84KK4
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The taxonomic range for the selected organisms is: Lotus japonicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
2-hydroxyisoflavanone 4'-o-methyltransferase, sam 2,7,4'-trihydroxyisoflavanone 4'-o-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SAM: 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
Specifically methylates 2,4',7-trihydroxyisoflavanone on the 4'-position. No activity with isoflavones [2]. The enzyme is involved in formononetin biosynthesis in legumes [1]. The protein from pea (Pisum sativum) also methylates (+)-6a-hydroxymaackiain at the 3-position (cf. EC 2.1.1.270, (+)-6a-hydroxymaackiain 3-O-methyltransferase) [4].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
show the reaction diagram
the enzyme is involved in formononetin biosynthesis in legumes
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
I4OMT_LOTJA
365
0
40690
Swiss-Prot
other Location (Reliability: 2)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akashi, T.; Sawada, Y.; Shimada, N.; Sakurai, N.; Aoki, T.; Ayabe, S.
cDNA cloning and biochemical characterization of S-adenosyl-L-methionine:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway
Plant Cell Physiol.
44
103-112
2003
Glycyrrhiza echinata (Q84KK6), Glycyrrhiza echinata, Lotus japonicus (Q84KK4), Lotus japonicus
Manually annotated by BRENDA team