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Information on EC 2.1.1.212 - 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase and Organism(s) Medicago truncatula and UniProt Accession Q29U70

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EC Tree
IUBMB Comments
Specifically methylates 2,4',7-trihydroxyisoflavanone on the 4'-position. No activity with isoflavones . The enzyme is involved in formononetin biosynthesis in legumes . The protein from pea (Pisum sativum) also methylates (+)-6a-hydroxymaackiain at the 3-position (cf. EC 2.1.1.270, (+)-6a-hydroxymaackiain 3-O-methyltransferase) .
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This record set is specific for:
Medicago truncatula
UNIPROT: Q29U70
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The taxonomic range for the selected organisms is: Medicago truncatula
The enzyme appears in selected viruses and cellular organisms
Synonyms
2-hydroxyisoflavanone 4'-o-methyltransferase, sam 2,7,4'-trihydroxyisoflavanone 4'-o-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
HI4'OMT/HM3OMT
bifunctional enzyme
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
Specifically methylates 2,4',7-trihydroxyisoflavanone on the 4'-position. No activity with isoflavones [2]. The enzyme is involved in formononetin biosynthesis in legumes [1]. The protein from pea (Pisum sativum) also methylates (+)-6a-hydroxymaackiain at the 3-position (cf. EC 2.1.1.270, (+)-6a-hydroxymaackiain 3-O-methyltransferase) [4].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
show the reaction diagram
additional information
?
-
low activity with dihydrodaidzein. 4'-Methoxy, 7-methoxy, and 4',7-dimethoxy products are formed in the reaction with dihydrodaidzein. No activity with: 6,7,4'-trihydroxyisoflavone, 6,7'-dihydroxy,4'-methoxyisoflavone, 7,3',4'-trihydroxyisoflavone, daidzein, genistein, glycitein, coumestrol, apigenin, 7,4'-dihydroxyflavone and myricetin
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2,7,4'-trihydroxyisoflavanone
S-adenosyl-L-homocysteine + 2,7-dihydroxy-4'-methoxyisoflavanone
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0733
2,7,4'-trihydroxyisoflavanone
pH 8.0, 30°C
0.0998
S-adenosyl-L-methionine
pH 8.0, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
334
2,7,4'-trihydroxyisoflavanone
pH 8.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
I4OMT_MEDTR
364
0
40754
Swiss-Prot
other Location (Reliability: 3)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystallized from a polyethylene glycol precipitant containing 2.5 mM of the reaction product S-adenosyl-L-homocysteine, 2.5 A resolution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Deavours, B.E.; Liu, C.J.; Naoumkina, M.A.; Tang, Y.; Farag, M.A.; Sumner, L.W.; Noel, J.P.; Dixon, R.A.
Functional analysis of members of the isoflavone and isoflavanone O-methyltransferase enzyme families from the model legume Medicago truncatula
Plant Mol. Biol.
62
715-733
2006
Medicago truncatula (Q29U70)
Manually annotated by BRENDA team
Liu, C.J.; Deavours, B.E.; Richard, S.B.; Ferrer, J.L.
Blount, J.W.; Huhman, D.; Dixon, R.A.; Noel, J.P.: Structural basis for dual functionality of isoflavonoid O-methyltransferases in the evolution of plant defense responses
Plant Cell
18
3656-3669
2006
Medicago truncatula (Q29U70)
Manually annotated by BRENDA team