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IUBMB Comments Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (guanine2535 N 1 EC 2.1.1.208 [23S rRNA (uridine2479 O )-methyltransferase], whose actions confer avilamycin resistance to the RNA.
Reaction Schemes
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guanine2535 in 23S rRNA
=
+
N1-methylguanine2535 in 23S rRNA
Synonyms
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AviRa
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S-adenosyl-L-methionine + guanine2535 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
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S-adenosyl-L-methionine:23S rRNA (guanine2535-N1)-methyltransferase
Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (guanine2535-N1)-methyltransferase and EC 2.1.1.208 [23S rRNA (uridine2479-2-O)-methyltransferase], whose actions confer avilamycin resistance to the RNA.
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S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
Substrates: AviRa modifies guanine2353 within helix 91 of 23S rRNA
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S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
Substrates: confers resistance to antibiotic avilamycin
?
S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
Substrates: AviRa modifies guanine2353 within helix 91 of 23S rRNA. The exact position of the modification is conformed by MALDI mass spectrometry, a single methyl group is added. No methylation activity with 16S rRNA
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S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
Substrates: AviRa modifies guanine2353 within helix 91 of 23S rRNA
?
S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
Substrates: confers resistance to antibiotic avilamycin
?
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strain Tü57
UniProt
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UniProt
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strain Tü57
UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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AVRA_STRVR
250
0
26636
Swiss-Prot
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hanging-drop vapour-diffusion method at 20°C. 1.5 A crystal structure
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I11SeMet/L239SeMet
as active as wild-type enzyme
I11SeMet/L246SeMet
as active as wild-type enzyme
L173SeMet/L239SeMet
as active as wild-type enzyme
L239SeMet/L246SeMet
as active as wild-type enzyme
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purified as fusion protein
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expressed in Streptomyces lividans TK66 or Escherichia coli
expression in Escherichia coli
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Weitnauer, G.; Gaisser, S.; Trefzer, A.; Stockert, S.; Westrich, L.; Quiros, L.M.; Mendez, C.; Salas, J.A.; Bechthold, A.
An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes T57
Antimicrob. Agents Chemother.
45
690-695
2001
Streptomyces viridochromogenes (Q9F5K5), Streptomyces viridochromogenes, Streptomyces viridochromogenes Tu57 (Q9F5K5)
brenda
Mosbacher, T.G.; Bechthold, A.; Schulz, G.E.
Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes
J. Mol. Biol.
329
147-157
2003
Streptomyces viridochromogenes (Q9F5K5), Streptomyces viridochromogenes
brenda
Treede, I.; Jakobsen, L.; Kirpekar, F.; Vester, B.; Weitnauer, G.; Bechthold, A.; Douthwaite, S.
The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose
Mol. Microbiol.
49
309-318
2003
Streptomyces viridochromogenes (Q9F5K5), Streptomyces viridochromogenes
brenda
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