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Information on EC 2.1.1.199 - 16S rRNA (cytosine1402-N4)-methyltransferase for references in articles please use BRENDA:EC2.1.1.199
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EC Tree
IUBMB Comments RsmH catalyses the N4-methylation of cytosine1402 and RsmI (EC 2.1.1.198) catalyses the 2'-O-methylation of cytosine1402 in 16S rRNA. Both methylations are necessary for efficient translation initiation at the UUG and GUG codons.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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cytosine1402 in 16S rRNA
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N4-methylcytosine1402 in 16S rRNA
Synonyms
methyltransferase RsmH, mraW, RsmH,
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RsmH
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA = S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
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S-adenosyl-L-methionine:16S rRNA (cytosine1402-N4)-methyltransferase
RsmH catalyses the N4-methylation of cytosine1402 and RsmI (EC 2.1.1.198) catalyses the 2'-O-methylation of cytosine1402 in 16S rRNA. Both methylations are necessary for efficient translation initiation at the UUG and GUG codons.
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytidine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytidine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
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?
S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytosine1402 in 16S rRNA
S-adenosyl-L-homocysteine + N4-methylcytosine1402 in 16S rRNA
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S-adenosyl-L-methionine
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the cofactor binds in the active site, structure, overview
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UniProt
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physiological function
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both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons
additional information
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RsmH in complex with S-adenosyl-L-methionine and cytidine consists of two distinct but structurally related domains: the typical MTase domain and the putative substrate recognition and binding domain. A deep pocket occurs in the conserved AdoMet binding domain, cytidine binds far from S-adenosyl-L-methionine with the distance of 25.9 A, The complex is not in a catalytically active state, and structural rearrangement of RsmH or the nucleotides neighboring C1402 may be necessary to trigger catalysis
malfunction
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strain DELTAmraW produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme
malfunction
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enzyme deletion affects efficiency of non-AUG initiation and the fidelity of translation
malfunction
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enzyme deletion affects efficiency of non-AUG initiation and the fidelity of translation
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malfunction
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enzyme deletion affects efficiency of non-AUG initiation and the fidelity of translation
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Escherichia coli (strain K12)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
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homodimer
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active enzyme in solution, structure analysis, overview
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purified recombinant His6-tagged RsmH in complex with S-adenosyl-L-methionine and cytidine in a 1:5:5 ratio, sitting drop vapour diffusion method, from 20 mM Tris, 100 mM NaCl, 5% v/v glycerol, 2 mM DTT, pH 8.0, room temperature, the best crystal is obtained in the mixture solution 1.0 M ammonium sulfate, 0.1 M HEPES, pH 6.5, and 0.5% w/v PEG 8, 000 in 3-4 days, X-ray diffraction structure determination and analysis at 2.25 A resolution
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recombinant His6-tagged RsmH from Escherichia coli strain BL21 DE3 star by nickel affinity chromatigraphy and gel filtration
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recombinant protein with N-terminal 6His-tags
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gene rsmH, expression of His6-tagged RsmH in Escherichia coli strain BL21 DE3 star
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Kimura, S.; Suzuki, T.
Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA
Nucleic Acids Res.
38
1341-1352
2010
Escherichia coli (P60390)
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Wei, Y.; Zhang, H.; Gao, Z.Q.; Wang, W.J.; Shtykova, E.V.; Xu, J.H.; Liu, Q.S.; Dong, Y.H.
Crystal and solution structures of methyltransferase RsmH provide basis for methylation of C1402 in 16S rRNA
J. Struct. Biol.
179
29-40
2012
Escherichia coli, Escherichia coli (P60390)
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Zhao, M.; Wang, L.; Zhang, H.; Dong, Y.; Gong, Y.; Zhang, L.; Wang, J.
Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli
Acta Crystallogr. Sect. F
70
1256-1259
2014
Escherichia coli
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Lioy, V.S.; Goussard, S.; Guerineau, V.; Yoon, E.J.; Courvalin, P.; Galimand, M.; Grillot-Courvalin, C.
Aminoglycoside resistance 16S rRNA methyltransferases block endogenous methylation, affect translation efficiency and fitness of the host
RNA
20
382-391
2014
Escherichia coli, Escherichia coli BW25113
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2.1.1.199 16S rRNA (cytosine1402-N4)-methyltransferase
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