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Information on EC 2.1.1.198 - 16S rRNA (cytidine1402-2'-O)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P67087

for references in articles please use BRENDA:EC2.1.1.198
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IUBMB Comments
RsmI catalyses the 2'-O-methylation of cytidine1402 and RsmH (EC 2.1.1.199) catalyses the N4-methylation of cytidine1402 in 16S rRNA. Both methylations are necessary for efficient translation initiation at the UUG and GUG codons.
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This record set is specific for:
Escherichia coli
UNIPROT: P67087
The expected taxonomic range for this enzyme is: Escherichia coli
The taxonomic range for the selected organisms is: Escherichia coli
Reaction Schemes
Synonyms
RsmI, YraL, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
YraL
290104
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:16S rRNA (cytidine1402-2'-O)-methyltransferase
RsmI catalyses the 2'-O-methylation of cytidine1402 and RsmH (EC 2.1.1.199) catalyses the N4-methylation of cytidine1402 in 16S rRNA. Both methylations are necessary for efficient translation initiation at the UUG and GUG codons.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
show the reaction diagram
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
-
-
?
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
show the reaction diagram
P67087
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
-
-
?
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
strain DELTAyraL produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme
physiological function
both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons
malfunction
-
enzyme deletion affects efficiency of non-AUG initiation and the fidelity of translation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
-
-
-
-
-
-
-
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
x * 31000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 31000, SDS-PAGE
CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 20% (w/v) PEG 3350
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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
recombinant protein with N-terminal 6His-tags
Ni-NTA column chromatography, heparin column chromatography, and Superdex 200 gel filtration
-
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) Star cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kimura, S.; Suzuki, T.
Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA
Nucleic Acids Res.
38
1341-1352
2010
Escherichia coli (P67087)
Manually annotated by BRENDA team
Zhao, M.; Wang, L.; Zhang, H.; Dong, Y.; Gong, Y.; Zhang, L.; Wang, J.
Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli
Acta Crystallogr. Sect. F
70
1256-1259
2014
Escherichia coli
Manually annotated by BRENDA team
Lioy, V.S.; Goussard, S.; Guerineau, V.; Yoon, E.J.; Courvalin, P.; Galimand, M.; Grillot-Courvalin, C.
Aminoglycoside resistance 16S rRNA methyltransferases block endogenous methylation, affect translation efficiency and fitness of the host
RNA
20
382-391
2014
Escherichia coli, Escherichia coli BW25113
Manually annotated by BRENDA team
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