Information on EC 2.1.1.198 - 16S rRNA (cytidine1402-2'-O)-methyltransferase

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The expected taxonomic range for this enzyme is: Escherichia coli

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EC NUMBER
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2.1.1.198
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RECOMMENDED NAME
GeneOntology No.
16S rRNA (cytidine1402-2'-O)-methyltransferase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:16S rRNA (cytidine1402-2'-O)-methyltransferase
RsmI catalyses the 2'-O-methylation of cytidine1402 and RsmH (EC 2.1.1.199) catalyses the N4-methylation of cytidine1402 in 16S rRNA. Both methylations are necessary for efficient translation initiation at the UUG and GUG codons.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
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LITERATURE
malfunction
physiological function
both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
show the reaction diagram
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
show the reaction diagram
P67087
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
show the reaction diagram
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
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LITERATURE
31000
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x * 31000, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
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LITERATURE
?
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x * 31000, SDS-PAGE
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 20% (w/v) PEG 3350
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, heparin column chromatography, and Superdex 200 gel filtration
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recombinant protein with N-terminal 6His-tags
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) Star cells
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Show AA Sequence (19225 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.198)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)