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Information on EC 2.1.1.198 - 16S rRNA (cytidine1402-2'-O)-methyltransferase for references in articles please use BRENDA:EC2.1.1.198
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IUBMB Comments RsmI catalyses the 2'-O-methylation of cytidine1402 and RsmH (EC 2.1.1.199) catalyses the N4-methylation of cytidine1402 in 16S rRNA. Both methylations are necessary for efficient translation initiation at the UUG and GUG codons.
The enzyme appears in viruses and cellular organisms
Reaction Schemes
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cytidine1402 in 16S rRNA
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2'-O-methylcytidine1402 in 16S rRNA
Synonyms
RsmI, YraL,
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RsmI
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
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S-adenosyl-L-methionine:16S rRNA (cytidine1402-2'-O)-methyltransferase
RsmI catalyses the 2'-O-methylation of cytidine1402 and RsmH (EC 2.1.1.199) catalyses the N4-methylation of cytidine1402 in 16S rRNA. Both methylations are necessary for efficient translation initiation at the UUG and GUG codons.
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine 1402 in 16S rRNA
the enzyme recognizes the 30S subunit as a substrate, but not the naked 16S rRNA or the tightly-coupled 70S ribosome
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?
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
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S-adenosyl-L-methionine + cytidine1402 in 16S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA
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SwissProt
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physiological function
both the 2'-O-methylation (catalyzed by RsmI) and N4-methylation of C1402 (catalyzed by RsmH) are needed for efficient initiation at the UUG and GUG codon. m4Cm1402 in the 16S rRNA modulates the accuracy of P-site function. The modification is needed for efficient translation initiation at the UUG and GUG codons
malfunction
strain DELTAyraL produces monomethylated cytosine1402 instead of dimethylated C1402 produced by the wild-type enzyme
malfunction
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enzyme deletion affects efficiency of non-AUG initiation and the fidelity of translation
malfunction
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enzyme deletion affects efficiency of non-AUG initiation and the fidelity of translation
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malfunction
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enzyme deletion affects efficiency of non-AUG initiation and the fidelity of translation
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Escherichia coli (strain K12)
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31000
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x * 31000, SDS-PAGE
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in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 20% (w/v) PEG 3350
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Ni-NTA column chromatography, heparin column chromatography, and Superdex 200 gel filtration
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recombinant protein with N-terminal 6His-tags
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expressed in Escherichia coli BL21 (DE3) Star cells
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Kimura, S.; Suzuki, T.
Fine-tuning of the ribosomal decoding center by conserved methyl-modifications in the Escherichia coli 16S rRNA
Nucleic Acids Res.
38
1341-1352
2010
Escherichia coli (P67087)
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Zhao, M.; Wang, L.; Zhang, H.; Dong, Y.; Gong, Y.; Zhang, L.; Wang, J.
Purification, crystallization and preliminary crystallographic analysis of the 16S rRNA methyltransferase RsmI from Escherichia coli
Acta Crystallogr. Sect. F
70
1256-1259
2014
Escherichia coli
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Lioy, V.S.; Goussard, S.; Guerineau, V.; Yoon, E.J.; Courvalin, P.; Galimand, M.; Grillot-Courvalin, C.
Aminoglycoside resistance 16S rRNA methyltransferases block endogenous methylation, affect translation efficiency and fitness of the host
RNA
20
382-391
2014
Escherichia coli, Escherichia coli BW25113
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2.1.1.198 16S rRNA (cytidine1402-2'-O)-methyltransferase
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