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Information on EC 2.1.1.191 - 23S rRNA (cytosine1962-C5)-methyltransferase for references in articles please use BRENDA:EC2.1.1.191Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Escherichia coli
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23S rRNA (cytosine1962-C5)-methyltransferase
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
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S-adenosyl-L-methionine:23S rRNA (cytosine1962-C5)-methyltransferase
The enzyme specifically methylates cytosine1962 at C5 in 23S rRNA.
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rRNA large subunit methyltransferase I
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RlmI
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YccW
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SwissProt
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SwissProt
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no activity in Staphylococcus aureus
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evolution
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YccW directed methylation at nucleotide C1962 is conserved in bacteria
evolution
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possible evolutionary relationships of the enzyme is discussed
malfunction
a mutant with a deletion of rlmI shows a significant decrease in biofilm formation
malfunction
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inactivation of the yccW gene leads to loss of methylation at nucleotide 1962 in Escherichia coli 23S rRNA. This methylation is restored by complementing the knockout strain with a plasmid-encoded copy of the yccW gene. Loss of this methylation in Escherichia coli results in a slight growth defect compared to wild-type cells during competitive growth experiments in rich or minimal medium
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
the enzyme can methylate naked 23S rRNA, but not assembled 50S ribosomal subunits or 70S ribosomes
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
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the enzyme specifically modifies Escherichia coli 23S rRNA at nucleotide C1962 to form 5-methylcytosine
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
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46500
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x * 46500, SDS-PAGE
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hanging-drop vapor-diffusion technique, crystal structure of RlmI at 2 A resolution, domain structure
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full-length YccW protein with a C-terminal histidine tag is expressed in Escherichia coli
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expression of rlmI increases 17fold upon deletion of tqsA (which increases biofilm formation)
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Herzberg, M.; Kaye, I.K.; Peti, W.; Wood, T.K.
YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport
J. Bacteriol.
188
587-598
2006
Escherichia coli K-12 (P75876)
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Purta, E.; O'Connor, M.; Bujnicki, J.M.; Douthwaite, S.
YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962
J. Mol. Biol.
383
641-651
2008
Escherichia coli (P75876)
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Sunita, S.; Tkaczuk, K.L.; Purta, E.; Kasprzak, J.M.; Douthwaite, S.; Bujnicki, J.M.; Sivaraman, J.
Crystal structure of the Escherichia coli 23S rRNA:m5C methyltransferase RlmI (YccW) reveals evolutionary links between RNA modification enzymes
J. Mol. Biol.
383
652-666
2008
Escherichia coli (P75876)
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Kita, S.; Tanaka, Y.; Hirano, N.; Kimura, S.; Suzuki, T.; Suzuki, T.; Yao, M.; Tanaka, I.
Crystal structure of a putative methyltransferase SAV1081 from Staphylococcus aureus
Protein Pept. Lett.
20
530-537
2013
no activity in Staphylococcus aureus
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