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IUBMB Comments The enzyme specifically methylates cytosine1962 C 5
Reaction Schemes
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cytosine1962 in 23S rRNA
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5-methylcytosine1962 in 23S rRNA
Synonyms
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rRNA large subunit methyltransferase I
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RlmI
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YccW
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
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S-adenosyl-L-methionine:23S rRNA (cytosine1962-C5)-methyltransferase
The enzyme specifically methylates cytosine1962 at C5 in 23S rRNA.
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
Substrates: the enzyme can methylate naked 23S rRNA, but not assembled 50S ribosomal subunits or 70S ribosomes
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
Substrates: the enzyme specifically modifies Escherichia coli 23S rRNA at nucleotide C1962 to form 5-methylcytosine
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S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
Substrates: -
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SwissProt
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SwissProt
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no activity in Staphylococcus aureus
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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evolution
YccW directed methylation at nucleotide C1962 is conserved in bacteria
evolution
possible evolutionary relationships of the enzyme is discussed
malfunction
inactivation of the yccW gene leads to loss of methylation at nucleotide 1962 in Escherichia coli 23S rRNA. This methylation is restored by complementing the knockout strain with a plasmid-encoded copy of the yccW gene. Loss of this methylation in Escherichia coli results in a slight growth defect compared to wild-type cells during competitive growth experiments in rich or minimal medium
malfunction
a mutant with a deletion of rlmI shows a significant decrease in biofilm formation
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46500
x * 46500, SDS-PAGE
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hanging-drop vapor-diffusion technique, crystal structure of RlmI at 2 A resolution, domain structure
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full-length YccW protein with a C-terminal histidine tag is expressed in Escherichia coli
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expression of rlmI increases 17fold upon deletion of tqsA (which increases biofilm formation)
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Herzberg, M.; Kaye, I.K.; Peti, W.; Wood, T.K.
YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport
J. Bacteriol.
188
587-598
2006
Escherichia coli K-12 (P75876)
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Purta, E.; O'Connor, M.; Bujnicki, J.M.; Douthwaite, S.
YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962
J. Mol. Biol.
383
641-651
2008
Escherichia coli (P75876)
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Sunita, S.; Tkaczuk, K.L.; Purta, E.; Kasprzak, J.M.; Douthwaite, S.; Bujnicki, J.M.; Sivaraman, J.
Crystal structure of the Escherichia coli 23S rRNA:m5C methyltransferase RlmI (YccW) reveals evolutionary links between RNA modification enzymes
J. Mol. Biol.
383
652-666
2008
Escherichia coli (P75876)
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Kita, S.; Tanaka, Y.; Hirano, N.; Kimura, S.; Suzuki, T.; Suzuki, T.; Yao, M.; Tanaka, I.
Crystal structure of a putative methyltransferase SAV1081 from Staphylococcus aureus
Protein Pept. Lett.
20
530-537
2013
no activity in Staphylococcus aureus
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