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Information on EC 2.1.1.187 - 23S rRNA (guanine745-N1)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P36999

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IUBMB Comments
The enzyme specifically methylates guanine745 at N1 in 23S rRNA.
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This record set is specific for:
Escherichia coli
UNIPROT: P36999
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
Synonyms
rlma(i), rlmai, 23s rrna m1g745 methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23S rRNA m1G745 methyltransferase
-
23S rRNA:m1G745 methyltransferase
-
ribosomal RNA(m1G)-methylase
-
RlmA
ambiguous
rRNA large subunit methyltransferase I
-
rRNA(m1G)methylase
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine745-N1)-methyltransferase
The enzyme specifically methylates guanine745 at N1 in 23S rRNA.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-25-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine745 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31500
2 * 31500
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 31500
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion techniques at 22°C, crystal structure of RlmAI at 2.8 A resolution. The crystal structure of RlmAI has a well defined and largely positively charged W-shaped RNA-binding cleft formed by asymmetric dimerization
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Isaksson, L.A.
Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)-methylases from Escherichia coli and demonstration of some proteins affecting their apparent activity
Biochim. Biophys. Acta
312
122-133
1973
Escherichia coli (P36999)
Manually annotated by BRENDA team
Hansen, L.H.; Kirpekar, F.; Douthwaite, S.
Recognition of nucleotide G745 in 23 S ribosomal RNA by the RrmA methyltransferase
J. Mol. Biol.
310
1001-1010
2001
Escherichia coli (P36999)
Manually annotated by BRENDA team
Liu, M.; Douthwaite, S.
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria
Mol. Microbiol.
44
195-204
2002
Acinetobacter sp. (Q9AEP4), Acinetobacter sp. ADP1 (Q9AEP4), Azotobacter vinelandii (C1DSW3), Dickeya chrysanthemi, Escherichia coli (P36999), Escherichia coli, Klebsiella aerogenes, Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri (A4VMZ0), Pseudomonas syringae (Q48F48), Shewanella putrefaciens
Manually annotated by BRENDA team
Bujnicki, J.M.; Blumenthal, R.M.; Rychlewski, L.
Sequence analysis and structure prediction of 23S rRNA:m1G methyltransferases reveals a conserved core augmented with a putative Zn-binding domain in the N-terminus and family-specific elaborations in the C-terminus
J. Mol. Microbiol. Biotechnol.
4
93-99
2002
Escherichia coli (P36999)
Manually annotated by BRENDA team
Das, K.; Acton, T.; Chiang, Y.; Shih, L.; Arnold, E.; Montelione, G.T.
Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site
Proc. Natl. Acad. Sci. USA
101
4041-4046
2004
Escherichia coli (P36999), Escherichia coli
Manually annotated by BRENDA team
Gustafsson, C.; Persson, B.C.
Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant
J. Bacteriol.
180
359-365
1998
Escherichia coli (P36999), Escherichia coli
Manually annotated by BRENDA team