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Information on EC 2.1.1.185 - 23S rRNA (guanosine2251-2'-O)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P63177

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IUBMB Comments
The enzyme catalyses the methylation of guanosine2251, a modification conserved in the peptidyltransferase domain of 23S rRNA.
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This record set is specific for:
Escherichia coli
UNIPROT: P63177
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
23s rrna methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23S rRNA methyltransferase
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rRNA Gm2251 methyltransferase
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanosine2251-2'-O)-methyltransferase
The enzyme catalyses the methylation of guanosine2251, a modification conserved in the peptidyltransferase domain of 23S rRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanosine2251 in 23S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine2251 in 23S rRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanosine2251 in 23S rRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine2251 in 23S rRNA
show the reaction diagram
the enzyme is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
RlmB displays a AdoMet binding site located in the divergent C-terminal half of the catalytic domain
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
a DELTArlmB mutant lacks 2'-O-methylguanosine in 23S rRNA, the mutant does not show any ribosome assembly defects and is not outgrown by a wild-type strain even after 120 cell mass doublings
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
2 * 29000
60000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 29000
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sparse matrix screening by hanging drop vapor diffusion, crystal structure of this 2'O-methyltransferase has been determined at 2.5 A resolution
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
a DELTArlmB mutant lacks 2'-O-methylguanosine in 23S rRNA, the mutant does not show any ribosome assembly defects and is not outgrown by a wild-type strain even after 120 cell mass doublings
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lvgren, J.M.; Wikstrm, P.M.
The rlmB gene is essential for formation of Gm2251 in 23S rRNA but not for ribosome maturation in Escherichia coli
J. Bacteriol.
183
6957-6960
2001
Escherichia coli (P63177)
Manually annotated by BRENDA team
Michel, G.; Sauve, V.; Larocque, R.; Li, Y.; Matte, A.; Cygler, M.
The structure of the RlmB 23S rRNA methyltransferase reveals a new methyltransferase fold with a unique knot
Structure
10
1303-1315
2002
Escherichia coli (P63177), Escherichia coli
Manually annotated by BRENDA team