We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.1.1.181 - 23S rRNA (adenine1618-N6)-methyltransferase for references in articles please use BRENDA:EC2.1.1.181Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The expected taxonomic range for this enzyme is: Escherichia coli
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
23S rRNA (adenine1618-N6)-methyltransferase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + adenine1618 in 23S rRNA = S-adenosyl-L-homocysteine + N6-methyladenine1618 in 23S rRNA
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine:23S rRNA (adenine1618-N6)-methyltransferase
The recombinant YbiN protein is able to methylate partially deproteinized 50 S ribosomal subunit, but neither the completely assembled 50 S subunits nor completely deproteinized 23 S rRNA [1].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
m6A1618 methyltransferase
-
rRNA large subunit methyltransferase F
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
malfunction
knockout of the ybiN gene leads to loss of modification at adenine1618. The modification is restored if ybiN knock-out strain has been co-transformed with a plasmid expressing the ybiN gene. Both lack of the ybiN gene and it's overexpression leads to growth retardation and loss of cell fitness comparative to the parental strain
physiological function
A1618 modification could be necessary for the exit tunnel interaction with some unknown regulatory peptides
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
-
-
-
?
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
the recombinant YbiN protein is able to methylate partially deproteinized 50 S ribosomal subunit, but neither the completely assembled 50 S subunit nor completely deproteinized 23 S rRNA
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + adenine1618 in 23S rRNA
S-adenosyl-L-homocysteine + 6-methyladenine1618 in 23S rRNA
P75782
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
34000
x * 34000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant YbiN carrying a N-terminal His6 tag is expressed in Escherichia coli and purified
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Sergiev, P.; Serebryakova, M.; Bogdanov, A.; Dontsova, O.
The ybiN gene of Escherichia coli encodes adenine-N6 methyltransferase specific for Modification of A1618 of 23 S ribosomal RNA, a methylated residue located close to the ribosomal exit tunnel
J. Mol. Biol.
375
291-300
2008
Escherichia coli, Escherichia coli (P75782)
brenda
html completed