Substrates: mitochondria of the yeast Saccharomyces cerevisiae assemble their ribosomes from ribosomal proteins, encoded by the nuclear genome (with one exception), and rRNAs of 15S and 21S, encoded by the mitochondrial genome. Unlike cytoplasmic rRNA, which is highly modified, mitochondrial rRNA contains only three modified nucleotides: a pseudouridine and two 2'-O-methylated riboses (Gm2270 and Um2791) located at the peptidyl transferase centre of 21S rRNA. Mrm2 is required for methylating U2791 of 21S rRNA Products: -
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S-adenosyl-L-methionine + uridine2791 in 21S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2791 in 21S rRNA
Substrates: Mrm2p methylates the 21S rRNA at position U2791 in vitro, when it is assembled with proteins into the ribosomal large subunit ir the precursor of the ribosomal large subunit. NO methylation is observed with deproteinized rRNA Products: -
Substrates: mitochondria of the yeast Saccharomyces cerevisiae assemble their ribosomes from ribosomal proteins, encoded by the nuclear genome (with one exception), and rRNAs of 15S and 21S, encoded by the mitochondrial genome. Unlike cytoplasmic rRNA, which is highly modified, mitochondrial rRNA contains only three modified nucleotides: a pseudouridine and two 2'-O-methylated riboses (Gm2270 and Um2791) located at the peptidyl transferase centre of 21S rRNA. Mrm2 is required for methylating U2791 of 21S rRNA Products: -
mitochondria of the yeast Saccharomyces cerevisiae assemble their ribosomes from ribosomal proteins, encoded by the nuclear genome (with one exception), and rRNAs of 15S and 21S, encoded by the mitochondrial genome. Unlike cytoplasmic rRNA, which is highly modified, mitochondrial rRNA contains only three modified nucleotides: a pseudouridine and two 2'-O-methylated riboses (Gm2270 and Um2791) located at the peptidyl transferase centre of 21S rRNA. Mrm2 is required for methylating U2791 of 21S rRNA
a knockout mutant shows a defect in respiration and the reduction of the 2'-O-methylmodification at position U2791 in the yeast mitochondrial 21S rRNA. Complementation with the Mrm2 allele carrying the yeast mutation G259R which is equivalent to G189R in the human gene fails to rescue the respiratory phenotype, which is instead completely rescued by expressing the wild-type allele
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