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Information on EC 2.1.1.167 - 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P25582

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IUBMB Comments
Spb1p is a site-specific 2'-O-ribose RNA methyltransferase that catalyses the formation of 2'-O-methylguanosine2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. 2'-O-Methylguanosine2922 is formed at a later stage of the processing, during the maturation of of the 27S pre-rRNA. In absence of snR52, Spb1p can also catalyse the formation of uridine2921 .
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Saccharomyces cerevisiae
UNIPROT: P25582
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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S-adenosyl-L-methionine
+
guanosine2922 in 27S pre-rRNA
=
S-adenosyl-L-homocysteine
+
2'-O-methylguanosine2922 in 27S pre-rRNA
+
guanosine2922 in 27S pre-rRNA
=
+
2'-O-methylguanosine2922 in 27S pre-rRNA
Synonyms
spb1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
Spb1p is a site-specific 2'-O-ribose RNA methyltransferase that catalyses the formation of 2'-O-methylguanosine2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. 2'-O-Methylguanosine2922 is formed at a later stage of the processing, during the maturation of of the 27S pre-rRNA. In absence of snR52, Spb1p can also catalyse the formation of uridine2921 [1].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA
show the reaction diagram
-
Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA
-
-
?
S-adenosyl-L-methionine + uridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
show the reaction diagram
-
the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
show the reaction diagram
the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation
-
-
?
S-adenosyl-L-methionine + guanosine2922 in 27S pre-rRNA
S-adenosyl-L-homocysteine + 2'-O-methylguanosine2922 in 27S pre-rRNA
show the reaction diagram
-
Spb1p is a site-specific 2'-O-ribose RNA MTase that catalyzes the formation of Gm2922, a universally conserved position of the catalytic center of the ribosome that is essential for translation. The enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation. 2'-O-methylguanosine2922 appears at a late processing stage, during the maturation of the 27S pre-rRNA
-
-
?
S-adenosyl-L-methionine + uridine2918 in 25S rRNA
S-adenosyl-L-homocysteine + 2'-O-methyluridine2918 in 25S rRNA
show the reaction diagram
-
the enzyme is required for proper assembly of pre-ribosomal particles during the biogenesis of the 60S ribosomal subunit. Specifically methylates the guanosine in position 2922 of the 25S rRNA at the stage of 27S pre-rRNA maturation. Methylates also the uridine in position 2921 in the absence of methylation of this residue guided by snoRNA snR52 at the stage of 35S pre-rRNA maturation
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
Spb1p possesses a putative S-adenosyl-L-methionine-binding domain, which is common to the S-adenosyl-L-methionine-dependent methyltransferases
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
96500
calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 96500, calculated from sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D52A
-
when the mutant protein is the sole source of Spb1p in the cell (strain YBL4637), it leads to a severe growth defect with a generation time of about 300 min. No formation of 2'-O-methylguanosine2922
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pintard, L.; Kressler, D.; Lapeyre, B.
Spb1p is a yeast nucleolar protein associated with Nop1p and Nop58p that is able to bind S-adenosyl-L-methionine in vitro
Mol. Cell. Biol.
20
1370-1381
2000
Saccharomyces cerevisiae (P25582), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Bonnerot, C.; Pintard, L.; Lutfalla, G.
Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast
Mol. Cell
12
1309-1315
2003
Saccharomyces cerevisiae (P25582), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Lapeyre, B.; Purushothaman, S.K.
Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage
Mol. Cell
16
663-669
2004
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Kressler, D.; Rojo, M.; Linder P.; Cruz, J.
Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae
Nucleic Acids Res.
27
4598-4608
1999
Saccharomyces cerevisiae (P25582), Saccharomyces cerevisiae
Manually annotated by BRENDA team