Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.1.1.165 - methyl halide transferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q6AWU6

for references in articles please use BRENDA:EC2.1.1.165
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.165 methyl halide transferase
IUBMB Comments
This enzyme contributes to the methyl halide emissions from Arabidopsis .
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q6AWU6
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
methyl chloride transferase, methyl halide transferase, athol3, athol2, s-adenosyl-l-methionine:halide/bisulfide methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosylmethionine:I- methyltransferase
This enzyme contributes to the methyl halide emissions from Arabidopsis [6].
CAS REGISTRY NUMBER
COMMENTARY hide
129877-08-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + chloride
S-adenosyl-L-homocysteine + methyl chloride
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + iodide
S-adenosyl-L-homocysteine + methyl iodide
show the reaction diagram
recombinant proteins methylate iodide with greater efficiency than chloride
-
-
?
S-adenosyl-L-methionine + chloride
S-adenosyl-L-homocysteine + methyl chloride
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + iodide
S-adenosyl-L-homocysteine + methyl iodide
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00009
production of methyl iodide
0.0000058
production of methyl iodide
205
production of methyl iodide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HOL3_ARATH
226
0
25043
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
53300
x * 53300, SDS-PAGE
53500
x * 53500, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 90% of the iodide methyltransferase activity
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 40% of the iodide methyltransferase activity
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 60% of the iodide methyltransferase activity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rhew, R.C.; Ostergaard, L.; Saltzman, E.S.; Yanofsky, M.F.
Genetic control of methyl halide production in Arabidopsis
Curr. Biol.
13
1809-1813
2003
Arabidopsis thaliana (Q0WP12)
Manually annotated by BRENDA team
Nagatoshi Y, Nakamura T.
Arabidopsis HARMLESS TO OZONE LAYER protein methylates a glucosinolate breakdown product and functions in resistance to Pseudomonas syringae pv. maculicola
J. Biol. Chem.
284
19301-19309
2009
Arabidopsis thaliana (Q0WP12)
Manually annotated by BRENDA team
Nagatoshi. Y.; Nakamura, T.
Characterization of three halide methyltransferases in Arabidopsis thaliana
Plant Biotechnol.
24
503-506
2007
Arabidopsis thaliana (O80562), Arabidopsis thaliana (Q0WP12), Arabidopsis thaliana (Q6AWU6)
-
Manually annotated by BRENDA team