Information on EC 2.1.1.161 - dimethylglycine N-methyltransferase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
2.1.1.161
-
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RECOMMENDED NAME
GeneOntology No.
dimethylglycine N-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine
show the reaction diagram
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
glycine betaine biosynthesis IV (from glycine)
-
-
glycine betaine biosynthesis V (from glycine)
-
-
Glycine, serine and threonine metabolism
-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:N,N-dimethylglycine N-methyltransferase (betaine-forming)
This enzyme, from the marine cyanobacterium Synechococcus sp. WH8102, differs from EC 2.1.1.157, sarcosine/dimethylglycine N-methyltransferase in that it cannot use sarcosine as an alternative substrate [1]. Betaine is a 'compatible solute' that enables cyanobacteria to cope with osmotic stress by maintaining a positive cellular turgor.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
show the reaction diagram
additional information
?
-
several other amino acids tested as substrates and non of them serves as methyl group acceptor
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
show the reaction diagram
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alanine
-
slight inhibition
cysteine
-
slight inhibition
isovaleric acid
-
slight inhibition
methionine
-
slight inhibition
n-butyric acid
-
-
N-methylglycine
-
slight inhibition
phenylalanine
-
slight inhibition
propionic acid
-
slight inhibition
S-adenosyl-L-homocysteine
-
-
t-butyrylacetic acid
-
-
additional information
-
no inhibition with betaine
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5 - 2.11
N,N-dimethylglycine
0.18 - 0.41
S-adenosyl-L-methionine
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5
with N,N-dimethylglycin as substrate
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11
-
very low activity below pH 7
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
-
gel filtration
31000
-
1 * 31000, SDS-PAGE, 1 * 29000, gel filtration
31210
-
calculated from the deduced amino acid sequence
32000
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration
34000
gel filtration
36000
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein using His-tag
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
expressed in Escherichia coli BL21(DE3)
-
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
M172R
-
almost inactive
P171Q
-
strongly increased Km for dimethylglycine, slight increase in the Km for S-adenosyl-L-methionine
P171Q/M172R
-
almost inactive
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.161)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)