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EC Tree
IUBMB Comments This enzyme, from the marine cyanobacterium Synechococcus sp. WH8102, differs from EC 2.1.1.157, sarcosine/dimethylglycine N-methyltransferase in that it cannot use sarcosine as an alternative substrate . Betaine is a 'compatible solute' that enables cyanobacteria to cope with osmotic stress by maintaining a positive cellular turgor.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
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S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine
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S-adenosyl-L-methionine:N,N-dimethylglycine N-methyltransferase (betaine-forming)
This enzyme, from the marine cyanobacterium Synechococcus sp. WH8102, differs from EC 2.1.1.157, sarcosine/dimethylglycine N-methyltransferase in that it cannot use sarcosine as an alternative substrate [1]. Betaine is a 'compatible solute' that enables cyanobacteria to cope with osmotic stress by maintaining a positive cellular turgor.
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S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
additional information
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several other amino acids tested as substrates and non of them serves as methyl group acceptor
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?
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
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?
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
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synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
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?
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
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-
?
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
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?
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S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
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synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
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?
S-adenosyl-L-methionine + N,N-dimethylglycine
S-adenosyl-L-homocysteine + N,N,N-trimethylglycine
synthesis of the compatible solute betaine de novo through the methylation of glycine, sarcosine and dimethylglycine with the methyl group from S-adenosylmethionine
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?
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alanine
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slight inhibition
cysteine
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slight inhibition
isovaleric acid
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slight inhibition
methionine
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slight inhibition
N-methylglycine
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slight inhibition
phenylalanine
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slight inhibition
propionic acid
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slight inhibition
S-adenosyl-L-homocysteine
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additional information
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no inhibition with betaine
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0.5 - 2.11
N,N-dimethylglycine
0.18 - 0.41
S-adenosyl-L-methionine
0.5
N,N-dimethylglycine
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pH 8.8, 37°C
2.11
N,N-dimethylglycine
pH 8.2, 37°C
0.18
S-adenosyl-L-methionine
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pH 8.8, 37°C
0.41
S-adenosyl-L-methionine
pH 8.2, 37°C
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8.5
with N,N-dimethylglycin as substrate
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7 - 11
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very low activity below pH 7
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brenda
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UniProt
brenda
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SDMT_SYNPX
Synechococcus sp. (strain WH8102)
280
0
30752
Swiss-Prot
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A0A163YNR4_9SYNE
285
0
31193
TrEMBL
-
A0A2L1CCD3_METMI
218
0
24456
TrEMBL
-
A0A1Y6MFX3_9GAMM
209
0
23788
TrEMBL
other Location (Reliability: 3 )
A0A1V5MBR6_9FIRM
193
0
21544
TrEMBL
-
A0A162DZC5_PROMR
275
0
30546
TrEMBL
-
A0A6C0PZE4_9ACTN
271
0
30315
TrEMBL
-
A0A163CWI6_9PROC
275
0
30516
TrEMBL
-
A0A163UJG3_PROMR
275
0
30589
TrEMBL
-
A0A3G4W1L5_9ACTN
224
0
24115
TrEMBL
-
U5DKV9_9CHRO
279
0
31110
TrEMBL
-
A0A1V5CR99_9DELT
247
0
26987
TrEMBL
-
A0A163ZQY6_9SYNE
285
0
31286
TrEMBL
-
A0A1T4U915_9GAMM
213
0
24302
TrEMBL
-
A0A2T7YM11_BACTU
209
0
24169
TrEMBL
-
A0A163EYJ7_9PROC
275
0
30527
TrEMBL
-
A0A1Z3HKJ2_9CYAN
258
1
30248
TrEMBL
-
A0A1V4WDK4_9DELT
244
0
26386
TrEMBL
-
A0A1Y5S9X2_9PROT
320
0
35192
TrEMBL
-
A0A518CUT6_9BACT
251
0
27283
TrEMBL
-
A0A163PWD2_PROMR
275
0
30530
TrEMBL
-
A0A3N6HFA8_9ACTN
284
0
30948
TrEMBL
-
A0A1Y2MXX7_PSEAH
245
0
26336
TrEMBL
-
A0A0F0LLV4_9MICO
195
0
20936
TrEMBL
-
A0A1Y6KUK2_9GAMM
209
0
23867
TrEMBL
-
A0A1V5CJL6_9DELT
244
0
26386
TrEMBL
-
A0A142WR62_9PLAN
254
0
28843
TrEMBL
-
A0A1B2GYU4_STRNR
290
0
32288
TrEMBL
-
A0A1T5HXB6_9GAMM
209
0
23962
TrEMBL
-
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31000
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1 * 31000, SDS-PAGE, 1 * 29000, gel filtration
31210
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calculated from the deduced amino acid sequence
32000
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration
36000
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration
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monomer
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1 * 31000, SDS-PAGE, 1 * 29000, gel filtration
monomer
1 * 36000, SDS-PAGE, 1 * 32000, calculated from the deduced amino acid sequence, 1 * 34000, gel fltration
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P171Q
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strongly increased Km for dimethylglycine, slight increase in the Km for S-adenosyl-L-methionine
P171Q/M172R
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almost inactive
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recombinant protein using His-tag
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expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS
expressed in Escherichia coli BL21(DE3)
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Waditee, R.; Tanaka, Y.; Aoki, K.; Hibino, T.; Jikuya, H.; Takano, J.; Takabe, T.
Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica
J. Biol. Chem.
278
4932-4942
2003
Aphanothece halophytica
brenda
Lu, W.D.; Chi, Z.M.; Su, C.D.
Identification of glycine betaine as compatible solute in Synechococcus sp. WH8102 and characterization of its N-methyltransferase genes involved in betaine synthesis
Arch. Microbiol.
186
495-506
2006
Synechococcus sp. WH 8102 (Q7U4Z9)
brenda
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Enzyme Nomenclature
2.1.1.161 dimethylglycine N-methyltransferase
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