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Information on EC 2.1.1.146 - (iso)eugenol O-methyltransferase
for references in articles please use BRENDA:EC2.1.1.146
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EC Tree 2 Transferases
2.1 Transferring one-carbon groups
2.1.1 Methyltransferases
2.1.1.146 (iso)eugenol O-methyltransferase
IUBMB Comments
Acts on eugenol and chavicol as well as isoeugenol.
The enzyme appears in viruses and cellular organisms
- 2.1.1.146
- breweri
-
clarkia
-
caffeic
-
ocimum
-
phenylpropene
- methyleugenol
-
phenylpropanoids
- basil
-
floral
-
scent
- basilicum
- trichomes
- flower
-
peltate
- monolignols
- lignin
-
glandular
- petal
-
coniferyl
-
para-hydroxyls
- aroma
-
onagraceae
-
o-methylation
-
chemotype
-
regiospecificity
-
emit
- california
-
etherifying
Reaction Schemes
showSynonyms
aimt1, chavicol o-methyltransferase, eugenol o-methyltransferase, rcomt1, monolignol 4-o-methyltransferase, (iso)eugenol o-methyltransferase, s-adenosyl-l-methionine:(iso)eugenol o-methyltransferase, isoeugenol-o-methyltransferase, eugenol-o-methyltransferase, momt5, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(iso)eugenol O-methyltransferase
-
-
-
-
allylphenol O-methyltransferase
-
-
-
-
chavicol O-methyltransferase
-
-
-
-
EOMT
eugenol methyltransferase
-
-
-
-
eugenol O-methyltransferase
-
-
-
-
IEMT
isoeugenol methyltransferase
-
-
-
-
methyltransferase, (iso)eugenol
-
-
-
-
methyltransferase, allylphenol O-
-
-
-
-
S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase
-
-
-
-
EOMT
-
-
-
-
IEMT
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + isoeugenol = S-adenosyl-L-homocysteine + isomethyleugenol
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
volatile cinnamoic ester biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:isoeugenol O-methyltransferase
Acts on eugenol and chavicol as well as isoeugenol.
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CAS REGISTRY NUMBER
COMMENTARY
191744-33-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2-methoxy-4-methylphenol
S-adenosyl-L-homocysteine + 1,2-dimethoxy-4-methylbenzene
-
Substrates: 16.8% of the activity with eugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + 2-methoxyphenol
S-adenosyl-L-homocysteine + ?
-
Substrates: 4% of the activity with eugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + 5-methoxyeugenol
S-adenosyl-L-homocysteine + ?
-
Substrates: about 18% of the activity with isoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + caffeyl alcohol
S-adenosyl-L-homocysteine + 4-methoxycaffeyl alcohol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + chavicol
S-adenosyl-L-homocysteine + estragol
Substrates: 24% of the activity with eugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + coniferyl alcohol
S-adenosyl-L-homocysteine + 4-methoxyconiferyl alcohol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + coumaryl alcohol
S-adenosyl-L-homocysteine + 4-methoxycoumaryl alcohol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + dihydrochavicol
S-adenosyl-L-homocysteine + ?
-
Substrates: about 23% of the activity with isoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + dihydroeugenol
S-adenosyl-L-homocysteine + methyldihydroeugenol
-
Substrates: about 50% of the activity with isoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + guaiacol
S-adenosyl-L-homocysteine + 1,2-dimethoxybenzene
Substrates: 29.9% of the activity with eugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + monolignol
S-adenosyl-L-homocysteine + 4-O-methylmonolignol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + sinapyl alcohol
S-adenosyl-L-homocysteine + 4-methoxysinapyl alcohol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + t-anol
S-adenosyl-L-homocysteine + t-anethole
-
Substrates: methylation of the p-OH group. 45% of the rate with isoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + trans-isoeugenol
S-adenosyl-L-homocysteine + (E)-2',3'-dimethoxy-1-propenylbenzene
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + zingerone
S-adenosyl-L-homocysteine + 4-(3,4-dimethoxyphenyl)-butan-2-one
-
Substrates: 26.8% of the activity with eugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
Substrates: the enzyme is involved in the production of the floral volatile compounds methyleugenol and methylisoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
Substrates: 26% of the activity with eugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
Substrates: the enzyme is involved in the production of the floral volatile compounds methyleugenol and methylisoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
Substrates: methylation of the p-OH group
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
Substrates: 75% of the activity with eugenol
Products: -
Products: -
?
additional information
?
-
Substrates: substrate specificities of engineered monolignol 4-O-methyltransferases, MOMT3, MOMT4, MOMT5, MOMT8, and MOMT9
Products: -
Products: -
-
additional information
?
-
-
Substrates: no substrate: chavicol, eugenol, guaiacol, caffeic acid, 2-propylphenol
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + eugenol
S-adenosyl-L-homocysteine + methyleugenol
-
Substrates: the enzyme is involved in the production of the floral volatile compounds methyleugenol and methylisoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + methylisoeugenol
-
Substrates: the enzyme is involved in the production of the floral volatile compounds methyleugenol and methylisoeugenol
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + isoeugenol
S-adenosyl-L-homocysteine + isomethyleugenol
Substrates: -
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0114
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT5
0.0161
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT8
0.0161
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT9
0.1926
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT4
0.1985
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT3
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.049
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT8
0.063
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT9
0.069
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT5
0.247
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT3
0.528
coniferyl alcohol
pH 7.5, 30Ā°C, recombinant enzyme mutant MOMT4
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0025
enzyme variant MOMT8, pH 7.5, 30Ā°C, substrate coumaryl alcohol
0.0037
enzyme variant MOMT9, pH 7.5, 30Ā°C, substrate coumaryl alcohol
0.0055
enzyme variant MOMT4, pH 7.5, 30Ā°C, substrate coumaryl alcohol
0.0096
enzyme variant MOMT5, pH 7.5, 30Ā°C, substrate coumaryl alcohol
0.0514
enzyme variant MOMT8, pH 7.5, 30Ā°C, substrate coniferyl alcohol
0.0668
enzyme variant MOMT4, pH 7.5, 30Ā°C, substrate coniferyl alcohol
0.0683
enzyme variant MOMT9, pH 7.5, 30Ā°C, substrate coniferyl alcohol
0.0806
enzyme variant MOMT5, pH 7.5, 30Ā°C, substrate coniferyl alcohol
0.3463
enzyme variant MOMT3, pH 7.5, 30Ā°C, substrate coniferyl alcohol
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
expression throughout the plant with highest level in developing fruit
brenda
additional information
-
expression throughout the plant with highest level in developing fruit
brenda
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
homology modeling and substrate docking analysis of enzyme mutant MOMT8, substrate binding in crystal structures of MOMTs, overview
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CVMT1_OCIBA
356
0
39916
Swiss-Prot
other Location (Reliability: 3)
EOMT1_OCIBA
357
0
40237
Swiss-Prot
other Location (Reliability: 4)
IEMT_CLABR
368
0
39993
Swiss-Prot
other Location (Reliability: 2)
OMT1_SORBI
376
0
40847
Swiss-Prot
other Location (Reliability: 2)
B9RW39_RICCO
351
0
38956
TrEMBL
other Location (Reliability: 3)
B9SGP0_RICCO
356
0
39868
TrEMBL
other Location (Reliability: 3)
B9RVR2_RICCO
175
0
19973
TrEMBL
other Location (Reliability: 4)
B9SGP3_RICCO
127
0
13881
TrEMBL
other Location (Reliability: 5)
A0A4D6DKF2_9LAMI
335
0
37639
TrEMBL
Secretory Pathway (Reliability: 5)
B9RGB0_RICCO
360
0
39427
TrEMBL
other Location (Reliability: 2)
A0A4D6DKB1_9LAMI
336
0
37562
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKK9_9LAMI
335
0
37274
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKA5_9LAMI
335
0
37355
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKA6_9LAMI
327
0
36673
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKJ4_9LAMI
336
0
37444
TrEMBL
Secretory Pathway (Reliability: 5)
B9T9S0_RICCO
110
0
12725
TrEMBL
Secretory Pathway (Reliability: 5)
B9RVR5_RICCO
362
0
40257
TrEMBL
other Location (Reliability: 2)
B9S932_RICCO
359
0
40513
TrEMBL
other Location (Reliability: 3)
B9TKY3_RICCO
228
0
25633
TrEMBL
Secretory Pathway (Reliability: 2)
B9SGP1_RICCO
356
0
39539
TrEMBL
other Location (Reliability: 3)
B9RVR4_RICCO
372
0
41854
TrEMBL
other Location (Reliability: 2)
B9SGP4_RICCO
321
0
35518
TrEMBL
other Location (Reliability: 1)
A0A4D6DKU0_9LAMI
335
0
37274
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKF9_9LAMI
334
0
37766
TrEMBL
Secretory Pathway (Reliability: 5)
B9SGP2_RICCO
132
0
14574
TrEMBL
other Location (Reliability: 5)
A0A4D6DKK0_9LAMI
331
0
36999
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKD2_OCIBA
335
0
37551
TrEMBL
Secretory Pathway (Reliability: 5)
B9S931_RICCO
359
0
40392
TrEMBL
other Location (Reliability: 3)
A0A4D6DK91_9LAMI
332
0
37191
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DK86_9LAMI
335
0
37483
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKH4_9LAMI
327
0
36705
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKE5_9LAMI
333
0
37132
TrEMBL
Secretory Pathway (Reliability: 5)
B9RW38_RICCO
352
0
39194
TrEMBL
other Location (Reliability: 4)
B9RVQ9_RICCO
105
0
11912
TrEMBL
other Location (Reliability: 2)
A0A4D6DKH0_9LAMI
333
0
37342
TrEMBL
Secretory Pathway (Reliability: 5)
A0A396JW13_MEDTR
101
0
11465
TrEMBL
other Location (Reliability: 3)
A0A2P6QQ71_ROSCH
366
0
40157
TrEMBL
other Location (Reliability: 2)
A0A4D6DKB6_9LAMI
336
0
37673
TrEMBL
Secretory Pathway (Reliability: 5)
B9RVQ8_RICCO
356
0
39607
TrEMBL
other Location (Reliability: 5)
A0A4D6DKD9_9LAMI
315
0
34928
TrEMBL
other Location (Reliability: 5)
A0A396GKB8_MEDTR
144
0
15899
TrEMBL
other Location (Reliability: 5)
A0A4D6DKG7_9LAMI
336
0
37510
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKB7_9LAMI
335
0
37288
TrEMBL
Secretory Pathway (Reliability: 5)
A0A4D6DKA0_9LAMI
335
0
37191
TrEMBL
Secretory Pathway (Reliability: 5)
B9RVR3_RICCO
362
0
40599
TrEMBL
other Location (Reliability: 2)