HOME
login
history
all enzymes
BRENDA home
History
Information on EC 2.1.1.128 - (RS)-norcoclaurine 6-O-methyltransferase
for references in articles please use BRENDA:EC2.1.1.128Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.1.1.128 (RS)-norcoclaurine 6-O-methyltransferaseIUBMB Comments
The enzyme will also catalyse the 6-O-methylation of (RS)-norlaudanosoline to form 6-O-methyl-norlaudanosoline, but this alkaloid has not been found to occur in plants.
Specify your search results
Word Map
- 2.1.1.128
- poppy
-
benzylisoquinoline
-
o-methyltransferases
-
opium
- berberine
-
coptis
- papaver
- japonica
-
4'-o-methyltransferase
- somniferum
-
4\'omt
-
3\'-hydroxylase
-
s-reticuline
- norreticuline
- papaverine
- codeinone
-
s-norcoclaurine
- isoquinoline
-
o-methylated
-
s-n-methylcoclaurine
-
branch-point
-
ping-pong
-
antispasmodic
- synthesis
-
polymethylated
- californica
- americanum
- flavum
-
aporphine
-
eschscholzia
-
regio-specificity
- cyp80b1
- sanguinarine
-
thalictrum
-
7-o-acetyltransferase
-
morphinan
- flavonols
-
phenylpropanoids
-
6-hydroxyl
- s-adenosyl-l-homocysteine
- medicine
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
(R,S)-norcoclaurine 6-O-methyltransferase, (S)-Coclaurine N-methyltransferase, (S)-norcoclaurine 6-O-methyltransferase, (S)-norcoclaurine-6-O-methyltransferase, 3’-hydroxy-N-methylcoclaurine 4’-O-methyltransferase, 6-OMT, 6OMT, 6OMT1, 6OMT2, 7OMT, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(R,S)-norcoclaurine 6-O-methyltransferase
(S)-Coclaurine N-methyltransferase
-
-
-
-
3’-hydroxy-N-methylcoclaurine 4’-O-methyltransferase
-
Ec4’OMT shows clear 6-O-methylation activity for norcoclaurine and produced coclaurine
6-OMT
6OMT
Methyltransferase, (S)-coclaurine N-
-
-
-
-
Methyltransferase, norlaudanosoline
-
-
-
-
NLS-OMT
-
-
-
-
Norcoclaurine 6-O-methyltransferase
S-Adenosyl-L-methionine:(S)-coclaurine-N-methyltransferase
-
-
-
-
S-Adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
S-Adenosylmethionine:(R),(S)-norlaudanosoline-6-O-methyltransferase
-
-
-
-
6-OMT
-
-
-
-
Norcoclaurine 6-O-methyltransferase
-
-
-
-
S-Adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + (RS)-norcoclaurine = S-adenosyl-L-homocysteine + (RS)-coclaurine
S-adenosyl-L-methionine + (RS)-norcoclaurine = S-adenosyl-L-homocysteine + (RS)-coclaurine
bi-bi ping-pong mechanism
-
S-adenosyl-L-methionine + (RS)-norcoclaurine = S-adenosyl-L-homocysteine + (RS)-coclaurine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
methyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(RS)-norcoclaurine 6-O-methyltransferase
The enzyme will also catalyse the 6-O-methylation of (RS)-norlaudanosoline to form 6-O-methyl-norlaudanosoline, but this alkaloid has not been found to occur in plants.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
167398-06-3
-
89700-33-4
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-homocysteine + (S)-reticuline
S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
-
-
?
S-Adenosyl-L-methionine + (R)-norlaudanosoline
S-Adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
S-Adenosyl-L-methionine + (R,S)-2,3-dihydroxy-9,10-dimethoxytetrahydroprotoberberine
S-Adenosyl-L-homocysteine + ?
-
5% of the activity with (S)-norlaudanosoline
-
-
?
S-Adenosyl-L-methionine + (R,S)-4'-O-methylnorlaudanosoline
S-Adenosyl-L-homocysteine + norprotosinomenine
-
34% of the activity with (S)-norlaudanosoline
-
?
S-Adenosyl-L-methionine + (R,S)-5'-O-methylnorlaudanosoline
S-Adenosyl-L-homocysteine + 6-O-methyllaudanosoline
-
81% of the activity with (S)-norlaudanosoline
-
?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
S-adenosyl-L-homocysteine + (RS)-6-O-methylnorlaudanosoline
-
-
-
-
?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + jatrorrhizine
-
1% of the activity with (S)-norlaudanosoline
-
?
S-Adenosyl-L-methionine + 2,3-dihydroxy-9,10-dimethoxyprotoberberine
S-Adenosyl-L-homocysteine + tetrahydrojatrorrhizine
-
7% of the activity with (S)-norlaudanosoline
-
?
S-Adenosyl-L-methionine + laudanosoline
S-Adenosyl-L-homocysteine + tetrahydrocolumbamine
-
79% of the activity with (S)-norlaudanosoline
-
?
S-Adenosyl-L-methionine + norcoclaurine
S-Adenosyl-L-homocysteine + ?
-
(S)-norcoclaurine and (R)-norcoclaurine
-
-
?
S-Adenosyl-L-methionine + (R)-norlaudanosoline
S-Adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
92% of the activity with (S)-norlaudanosoline
6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline in the ratio 8:2
?
S-Adenosyl-L-methionine + (R)-norlaudanosoline
S-Adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
-
-
-
?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
-
-
-
?
S-adenosyl-L-methionine + (R,S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
-
-
-
?
S-adenosyl-L-methionine + (RS)-norcoclaurine
S-adenosyl-L-homocysteine + (RS)-coclaurine
-
-
-
-
?
S-adenosyl-L-methionine + (RS)-norcoclaurine
S-adenosyl-L-homocysteine + (RS)-coclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
-
ir
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
preferred substrate
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
-
-
-
?
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
-
-
-
?
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
-
6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline in the ratio 8:2
?
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline + 7-O-methylnorlaudanosoline
-
activity with (R,S)-norlaudanosoline is 76% of the activity with (S)-norcoclaurine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-homocysteine + (S)-reticuline
S-adenosyl-L-methionine + 3'-hydroxy-N-methyl-(S)-coclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-norlaudanosoline
S-adenosyl-L-homocysteine + 6-O-methylnorlaudanosoline
-
putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (RS)-norcoclaurine
S-adenosyl-L-homocysteine + (RS)-coclaurine
-
-
-
-
?
S-adenosyl-L-methionine + (RS)-norcoclaurine
S-adenosyl-L-homocysteine + (RS)-coclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis
-
-
?
S-adenosyl-L-methionine + (S)-norcoclaurine
S-adenosyl-L-homocysteine + (S)-coclaurine
-
putative rate-limiting step enzymes in benzylisoquinoline alkaloid biosynthesis
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5,6-Dihydro-9,10-dimethoxybenzo[g]-1,3-benzodioxolo[5,6-a]quinolizium
-
10 mM, 50% inhibition
additional information
-
not inhibited by chloromercuribenzenesulfonate and iodoacetamide
-
additional information
-
not inhibitory: berberine up to 1 mM
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
WRKY1
-
CjWRKY1 is a necessary regulator to control overall gene expression in berberine biosynthesis
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.18
S-adenosyl-L-homocysteine
Coptis japonica
-
using (S)-norlaudanosoline as cosubstrate, in 0.3 M Tris-HCl (pH 7.5), 25mM sodium ascorbate, at 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
sieve elements of the phloem adjacent or proximal to laticifers
brenda
additional information
-
gene transcripts detected in all organs with highest levels in root and stem and lowest in leaf
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
immunogold labelling studies show the strict association with electron dense regions of the peripheral cytoplasm of sieve elements
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
suppression of norcoclaurine 6-O-methyltransferase transcript levels significantly suppresses total alkaloid accumulation in opium poppy (73% compared to the controls). However, the relative abundance of morphine increased to 55% of the total alkaloid content. Suppression of the enzyme does not significantly affect (S)-3'-hydroxy-N-methylcoclaurine 1 or (S)-3'-hydroxy-N-methylcoclaurine 2 transcript levels
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). 6OMT_COPJA
347
0
38700
Swiss-Prot
Secretory Pathway (Reliability: 3)
6OMT_PAPSO
346
0
38511
Swiss-Prot
other Location (Reliability: 3)
6OMT_THLFG
Yellow meadow rue
350
0
39385
Swiss-Prot
other Location (Reliability: 5)
6OMT_THLFG
350
0
39385
Swiss-Prot
other Location (Reliability: 5)
B9TM05_RICCO
98
0
11062
TrEMBL
other Location (Reliability: 2)
A0A0B4VG62_SINHE
350
0
39221
TrEMBL
Secretory Pathway (Reliability: 4)
A0A0B4VGY7_PODPE
373
0
41941
TrEMBL
other Location (Reliability: 1)
B9SUY0_RICCO
353
0
38999
TrEMBL
other Location (Reliability: 1)
B9TKC4_RICCO
98
0
11209
TrEMBL
other Location (Reliability: 2)
B9SUX9_RICCO
110
0
12590
TrEMBL
Secretory Pathway (Reliability: 5)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PDB
SCOP
CATH
UNIPROT
ORGANISM
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40000
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structures of apo-6OMT, the binary complex 6OMT/SAH and the two ternary complexes 6OMT/SAH/norlaudanosoline and 6OMT/SAH/sanguinarine, refined to a resolution of 2.6, 1.9, 1.6 and 1.8 A, respectively
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid
expression in Escherichia coli
full-length cDNA of Coptis japonica 6OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens
into the pET-21d vector for expression in Escherichia coli BL21DE3 cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is upregulated in the high papaverine mutant pap1
the over-expression of regulatory factors AP2G, AN1-like, ERF2, GARP, MDB025 and WRKY1 increases the levels of codeinone reductase, (S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase) and (R,S)-norcoclaurine 6-O-methyltransferase transcripts by 10- to more than 100fold. The transcriptional activations translate into an enhancement of alkaloid production in opium poppy of up to at least 10fold
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
a Saccharomyces cerevisiae strain is engineered to express seven heterologous enzymes (Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR)), resulting in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor. A number of strategies are implemented to improve flux through the pathway, including enzyme variant screening, genetic copy number variation, and culture optimization. This leads to an over 70-fold increase in canadine titer up to 1.8 mg/l. Increased canadine titers enable extension of the pathway to produce berberine, a major constituent of several traditional medicines in a microbial host. This strain is viable at pilot scale
synthesis
synthesis
-
production of the economically important analgesic morphine and the antimicrobial agent berberine
synthesis
production of the economically important analgesic morphine and the antimicrobial agent berberine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rueffer, M.; Nagakura, N.; Zenk, M.H.
Partial purification and properties of S-adenosylmethionine:(R),(S)-norlaudanosoline-6-O-methyltransferase from Argemone platyceras cell culture
J. Med. Plant Res.
49
131-137
1983
Argemone platyceras, Papaver somniferum
brenda
Sato, F.; Tsujita, T.; Katagiri, Y.; Yoshida, S.; Yamada, Y.
Purification and characterization of S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase from cultured Coptis japonica cells
Eur. J. Biochem.
225
125-131
1994
Coptis japonica
brenda
Samanani, N.; Alcantara, J.; Bourgault, R.; Zulak, K.G.; Facchini, P.J.
The role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy
Plant J.
47
547-563
2006
Papaver somniferum
brenda
Inui, T.; Tamura, K.; Fujii, N.; Morishige, T.; Sato, F.
Overexpression of Coptis japonica norcoclaurine 6-O-methyltransferase overcomes the rate-limiting step in Benzylisoquinoline alkaloid biosynthesis in cultured Eschscholzia californica
Plant Cell Physiol.
48
252-262
2007
Eschscholzia californica, Coptis japonica (Q9LEL6), Coptis japonica
brenda
Apuya, N.R.; Park, J.H.; Zhang, L.; Ahyow, M.; Davidow, P.; Van Fleet, J.; Rarang, J.C.; Hippley, M.; Johnson, T.W.; Yoo, H.D.; Trieu, A.; Krueger, S.; Wu, C.Y.; Lu, Y.P.; Flavell, R.B.; Bobzin, S.C.
Enhancement of alkaloid production in opium and California poppy by transactivation using heterologous regulatory factors
Plant Biotechnol. J.
6
160-175
2008
Papaver somniferum
brenda
Kato, N.; Dubouzet, E.; Kokabu, Y.; Yoshida, S.; Taniguchi, Y.; Dubouzet, J.G.; Yazaki, K.; Sato, F.
Identification of a WRKY protein as a transcriptional regulator of benzylisoquinoline alkaloid biosynthesis in Coptis japonica
Plant Cell Physiol.
48
8-18
2007
Coptis japonica
brenda
Desgagne-Penix, I.; Facchini, P.
Systematic silencing of benzylisoquinoline alkaloid biosynthetic genes reveals the major route to papaverine in opium poppy
Plant J.
72
331-344
2012
Papaver somniferum
brenda
Morishige, T.; Tamakoshi, M.; Takemura, T.; Sato, F.
Molecular characterization of O-methyltransferases involved in isoquinoline alkaloid biosynthesis in Coptis japonica
Proc. Jpn. Acad. Ser. B
86
757-768
2010
Coptis japonica
brenda
Pathak, S.; Lakhwani, D.; Gupta, P.; Mishra, B.; Shukla, S.; Asif, M.; Trivedi, P.
Comparative transcriptome analysis using high papaverine mutant of Papaver somniferum reveals pathway and uncharacterized steps of papaverine biosynthesis
PLoS ONE
8
e65622
2013
Papaver somniferum (Q6WUC1), Papaver somniferum
brenda
Galanie, S.; Smolke, C.
Optimization of yeast-based production of medicinal protoberberine alkaloids
Microb. Cell Fact.
14
144
2015
Papaver somniferum (Q6WUC1)
brenda
He, S.; Liang, Y.; Cong, K.; Chen, G.; Zhao, X.; Zhao, Q.; Zhang, J.; Wang, X.; Dong, Y.; Yang, J.; Zhang, G.; Qian, Z.; Fan, W.; Yang, S.
Identification and characterization of genes involved in benzylisoquinoline alkaloid biosynthesis in coptis species
Front. Plant Sci.
9
731
2018
Coptis chinensis (A0A2Z5FRS1), Coptis chinensis (A0A2Z5FRS5), Coptis teeta (A0A2Z5FRT2)
brenda
Robin, A.Y.; Giustini, C.; Graindorge, M.; Matringe, M.; Dumas, R.
Crystal structure of norcoclaurine-6-O-methyltransferase, a key rate-limiting step in the synthesis of benzylisoquinoline alkaloids
Plant J.
87
641-653
2016
Thalictrum flavum subsp. glaucum
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 2.1.1.128)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Information
