We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 2.1.1.117 - (S)-scoulerine 9-O-methyltransferase for references in articles please use BRENDA:EC2.1.1.117
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The product of this reaction is a precursor for protoberberine alkaloids in plants
The enzyme appears in viruses and cellular organisms
Synonyms
(S)-Adenosyl-L-methionine:(S)-scoularine 9-O-methyltransferase, (S)-scoulerine 9-O-methyltransferase, Methyltransferase, (S)-scoularine 9-, Methyltransferase, (S)-scoularine 9- (Coptis japonica clone pCJSMT), scoulerine 9-O-methyltransferase, scoulerine-9-O-methyltransferase, SMT, SOMT1,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-Adenosyl-L-methionine:(S)-scoularine 9-O-methyltransferase
-
-
-
-
(S)-scoulerine 9-O-methyltransferase
-
Methyltransferase, (S)-scoularine 9-
-
-
-
-
Methyltransferase, (S)-scoularine 9- (Coptis japonica clone pCJSMT)
-
-
-
-
scoulerine 9-O-methyltransferase
scoulerine-9-O-methyltransferase
-
-
scoulerine 9-O-methyltransferase
-
-
scoulerine 9-O-methyltransferase
-
-
SMT
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + (S)-scoulerine = S-adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
methyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine:(S)-scoulerine 9-O-methyltransferase
The product of this reaction is a precursor for protoberberine alkaloids in plants
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
164205-21-4
methyltransferase, (S)-scoularine 9- (Coptis japonica clone pCJSMT)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-Adenosyl-L-methionine + (R,S)-2,3,9,10-tetrahydroxytetrahydroprotoberberine
?
-
methylated at 50% of the rate of (S)-scoulerine
-
-
-
S-Adenosyl-L-methionine + (S)-3,9-dihydroxy-2,10-dimethoxytetrahydroprotoberberine
?
-
methylated at 4% of the rate of (S)-scoulerine
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
?
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
S-Adenosyl-L-methionine + 6-O-methylnorlaudanosoline
?
-
8% of the activity relative to (S)-scoulerine
-
-
-
S-Adenosyl-L-methionine + norlaudanosoline
?
-
2% of the activity relative to (S)-scoulerine
-
-
-
S-Adenosyl-L-methionine + norreticuline
?
-
9% of the activity relative to (S)-scoulerine
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
?
-
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
?
-
central role in orotoberberine biosynthesis
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
?
-
the enzyme is responsible for a late step in the biosynthesis of berberine
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
ir
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
?
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-Adenosyl-L-methionine + (S)-scoulerine
?
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
S-Adenosyl-L-methionine + (S)-scoulerine
?
-
-
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
?
-
central role in orotoberberine biosynthesis
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
?
-
the enzyme is responsible for a late step in the biosynthesis of berberine
-
-
-
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
E0W6R9
-
-
-
ir
S-Adenosyl-L-methionine + (S)-scoulerine
S-Adenosyl-L-homocysteine + (S)-tetrahydrocolumbamine
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Berberine
-
5 mM, 70% inhibition
Ca2+
-
1 mM, 64% inhibition
Mn2+
-
1 mM, 59% inhibition
palmatine
-
5 mM, 70% inhibition
PCMB
-
0.5 mM, 51% inhibition
Cu2+
-
5 mM, 97% inhibition
Cu2+
-
1 mM, 72% inhibition
S-adenosylhomocysteine
-
-
S-adenosylhomocysteine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.042 - 0.17
S-adenosyl-L-methionine
0.042
S-adenosyl-L-methionine
-
-
0.17
S-adenosyl-L-methionine
-
-
0.1
scoulerine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.5 - 10
-
50% of maximal activity at pH 7.5 and at pH 10
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
var. subcauliata
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
-
UniProt
brenda
-
-
-
brenda
transient RNA silencing of scoulerine 9-O-methyltransferase expression by double stranded RNA in Coptis japonica protoplasts
Uniprot
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
low activity
brenda
-
high activity; lateral; low activity; main root
brenda
-
high activity
brenda
-
-
brenda
-
activity of high berberine-producing cells is higher than that of non-selected cells
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
-
the enzyme is involved in berberine biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SMT_COPJA
381
0
41665
Swiss-Prot
SOMT1_PAPSO
390
0
42699
Swiss-Prot
SOMT2_PAPSO
356
0
39087
Swiss-Prot
SOMT3_PAPSO
339
0
37687
Swiss-Prot
OMT2B_PAPSO
356
0
39163
Swiss-Prot
OMT3B_PAPSO
339
0
37691
Swiss-Prot
A0A2P6QGQ1_ROSCH
116
0
13527
TrEMBL
A0A2P6Q5T8_ROSCH
361
0
40736
TrEMBL
Q5C9L2_THLFG
355
0
38591
TrEMBL
A0A2P6PAG7_ROSCH
364
0
40324
TrEMBL
A0A2P6PA32_ROSCH
362
0
40180
TrEMBL
A0A2P6PEX7_ROSCH
365
0
40072
TrEMBL
A0A2P6QE06_ROSCH
169
0
18683
TrEMBL
A0A1P7ZIM6_ARGME
277
0
30390
TrEMBL
E0W6R9_9MAGN
350
0
38484
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
38364
-
x * 38364, calculation from nucleotide sequence
41000
-
x * 41000, SDS-PAGE
120000 - 140000
-
gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 41000, SDS-PAGE
?
-
x * 38364, calculation from nucleotide sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-20°C, 20% glycerol, 50% loss of activity after 7 days
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid
expressed in Eschscholzia californica cultivar Hitoezaki cells
-
expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
analysis
transient RNA silencing of scoulerine 9-O-methyltransferase expression by double stranded RNA in Coptis japonica protoplasts. Utility of gene silencing based on in vitro synthesized dsRNA to study function and behavior of endogenous enzymes
medicine
a Saccharomyces cerevisiae strain is engineered to express seven heterologous enzymes (Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR)), resulting in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor. A number of strategies are implemented to improve flux through the pathway, including enzyme variant screening, genetic copy number variation, and culture optimization. This leads to an over 70-fold increase in canadine titer up to 1.8 mg/l. Increased canadine titers enable extension of the pathway to produce berberine, a major constituent of several traditional medicines in a microbial host. This strain is viable at pilot scale
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Fujiwara, H.; Takeshita, N.; Terano, Y.; Fitchen, J.H.; Tsujita, T.; Katagiri, Y.; Sato, F.; Yamada, Y.
Expression of (S)-scoulerine 9-O-methyltransferase in Coptis japonica plants
Phytochemistry
34
949-954
1993
Coptis japonica
-
brenda
Sato, F.; Takeshita, N.; Fitchen, J.H.; Fujiwara, H.; Yamada, Y.
S-Adenosyl-L-methionine:scoulerine-9-O-methyltransferase from cultured Coptis japonica cells
Phytochemistry
32
659-664
1993
Coptis japonica
-
brenda
Takeshita, N.; Fujiwara, H.; Mimura, H.; Fitchen, J.H.; Yamada, Y.; Sato, F.
Molecular cloning and characterization of S-adenosyl-L-methionine:scoulerine 9-O-methyltransferase from cultured cells of Coptis japonica
Plant Cell Physiol.
36
29-36
1995
Coptis japonica
brenda
Galneder, E.; Rueffer, M.; Wanner, G.; Tabata, M.; Zenk, M.H.
Alternative final steps in berberine biosynthesis in Coptis japonica cell cultures
Plant Cell Rep.
7
1-4
1988
Berberis stolonifera, Coptis japonica
brenda
Muemmler, S.; Rueffer, M.; Nagakura, N.; Zenk, M.H.
S-Adenosyl-L-methionine:(S)-scoulerine 9-O-methyltransferase, a highly stereo- and regio-specific enzyme in tetrahydroprotoberberine biosynthesis
Plant Cell Rep.
4
36-39
1985
Berberis wilsoniae
brenda
Dubouzet, J.G.; Morishige, T.; Fujii, N.; An, C.I.; Fukusaki, E.; Ifuku, K.; Sato, F.
Transient RNA silencing of scoulerine 9-O-methyltransferase expression by double stranded RNA in Coptis japonica protoplasts
Biosci. Biotechnol. Biochem.
69
63-70
2005
Coptis japonica, Coptis japonica (Q39522)
brenda
Takemura, T.; Ikezawa, N.; Iwasa, K.; Sato, F.
Metabolic diversification of benzylisoquinoline alkaloid biosynthesis through the introduction of a branch pathway in Eschscholzia californica
Plant Cell Physiol.
51
949-959
2010
Coptis japonica
brenda
Dang, T.T.; Facchini, P.J.
Cloning and characterization of canadine synthase involved in noscapine biosynthesis in opium poppy
FEBS Lett.
588
198-204
2014
Papaver somniferum
brenda
Zhu, Q.; Zhou, J.; Zhang, G.; Liao, H.
Homology modeling and molecular docking studies of (S)-scoulerine 9-O-methyltransferase from Coptis chinensis
Chin. J. Chem.
30
2533-2538
2012
Coptis chinensis (E0W6R9)
-
brenda
Galanie, S.; Smolke, C.
Optimization of yeast-based production of medicinal protoberberine alkaloids
Microb. Cell Fact.
14
144
2015
Thalictrum flavum subsp. glaucum (Q5C9L2)
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
2.1.1.117 (S)-scoulerine 9-O-methyltransferase
more
top
Information
