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Information on EC 2.1.1.109 - demethylsterigmatocystin 6-O-methyltransferase and Organism(s) Aspergillus parasiticus and UniProt Accession Q9UQY0

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IUBMB Comments
Dihydrodemethylsterigmatocystin can also act as acceptor. Involved in the biosynthesis of aflatoxins in fungi.
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This record set is specific for:
Aspergillus parasiticus
UNIPROT: Q9UQY0
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The taxonomic range for the selected organisms is: Aspergillus parasiticus
The enzyme appears in selected viruses and cellular organisms
Synonyms
o-methyltransferase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyltransferase, demethylsterigmatocystin
-
-
-
-
O-methyltransferase I
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:6-demethylsterigmatocystin 6-O-methyltransferase
Dihydrodemethylsterigmatocystin can also act as acceptor. Involved in the biosynthesis of aflatoxins in fungi.
CAS REGISTRY NUMBER
COMMENTARY hide
123516-47-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 6-demethylsterigmatocystin
S-adenosyl-L-homocysteine + sterigmatocystin
show the reaction diagram
S-adenosyl-L-methionine + dihydrodemethylsterigmatocystin
S-adenosyl-L-homocysteine + dihydrosterigmatocystin
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 6-demethylsterigmatocystin
S-adenosyl-L-homocysteine + sterigmatocystin
show the reaction diagram
-
involved in biosynthesis of aflatoxins in fungi
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-demethylsterigmatocystin
-
at higher concentrations
dihydrodemethylsterigmatocystin
-
at higher concentrations
N-ethylmaleimide
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00094
6-demethylsterigmatocystin
-
-
0.0025
dihydrodemethylsterigmatocystin
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.108
6-demethylsterigmatocystin
-
-
0.171
dihydrodemethylsterigmatocystin
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.043
-
with dihydrodemethylsterigmatocystin
0.061
-
with 6-demethylsterigmatocystin
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15000
-
gel filtration
43000
-
x * 43000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 43000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
phenyl-Sepharose, Sephacryl S-300, Matrex gel Green A
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yabe, K.; Ando, Y.; Hashimoto, J.; Hamasaki, T.
Two distinct O-methyltransferases in aflatoxin biosynthesis
Appl. Environ. Microbiol.
55
2172-2177
1989
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Manually annotated by BRENDA team
Yabe, K.; Matsushima, K.I.; Koyama, T.; Hamasaki, T.
Purification and characterization of O-methyltransferase I involved in conversion of demethylsterigmatocystin to sterigmatocystin and of dihydrodemethylsterigmatocystin to dihydrosterigmatocystin during aflatoxin biosynthesis
Appl. Environ. Microbiol.
64
166-171
1998
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Manually annotated by BRENDA team
Motomura, M.; Chihaya, N.; Shinozawa, T.; Hamasaki, T.; Yabe, K.
Cloning and characterization of the O-methyltransferase I gene (dmtA) from Aspergillus parasiticus associated with the conversions of demethylsterigmatocystin to sterigmatocystin and dihydrodemethylsterigmatocystin to dihydrosterigmatocystin in aflatoxin biosynthesis
Appl. Environ. Microbiol.
65
4987-4994
1999
Aspergillus parasiticus (Q9UQY0), Aspergillus parasiticus
Manually annotated by BRENDA team