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Information on EC 2.1.1.104 - caffeoyl-CoA O-methyltransferase and Organism(s) Triticum aestivum and UniProt Accession Q38J50

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.104 caffeoyl-CoA O-methyltransferase
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Select one or more organisms in this record: ?
This record set is specific for:
Triticum aestivum
UNIPROT: Q38J50 not found.
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Word Map
The taxonomic range for the selected organisms is: Triticum aestivum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
o-methyltransferase, ccoaomt, ccoaomt1, caffeic acid 3-o-methyltransferase, caffeoyl-coa o-methyltransferase, ccomt, ccoaomt2, ccoaomt-like, caffeoyl coa 3-o-methyltransferase, caffeoyl-coa 3-o-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
caffeic acid 3-O-methyltransferase
-
caffeoyl coenzyme A 3-O-methyltransferase
-
-
caffeoyl coenzyme A methyltransferase
-
-
-
-
caffeoyl-CoA 3-O-methyltransferase
-
-
-
-
TaCCoAOMT1
-
-
trans-caffeoyl-CoA 3-O-methyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:caffeoyl-CoA 3-O-methyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
120433-42-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + sinapaldehyde
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 4-coumaroyl-CoA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
very low activity
-
-
?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
show the reaction diagram
S-adenosyl-L-methionine + 5-hydroxyferuoyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
show the reaction diagram
S-adenosyl-L-methionine + feruloyl-CoA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
very low activity
-
-
?
S-adenosyl-L-methionine + sinapoyl-CoA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
low activity
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
S-adenosyl-L-methionine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0173
5-hydroxy-coniferaldehyde
-
0.0952
5-hydroxy-feruloyl-CoA
-
0.0688
caffeic acid
-
0.0437
caffeoyl aldehyde
-
0.083
caffeoyl-CoA
-
0.00223
5-hydroxy-feruloyl-CoA
-
pH 7.5, 30°C, recombinant enzyme
0.0022
5-hydroxyferuoyl-CoA
-
pH 7.5, 30°C
0.112 - 0.1123
caffeoyl-CoA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
40% of maximum activity
6.5 - 9.5
-
the enzyme has a very narrow pH range, it is most active at pH 7.5, and inactive below pH 6.5 and above pH 9.5
8.5
-
40% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 35
-
over 50% of maximal activity within this range, profile overview
40
-
40% of maximum activity
50
-
almost complete loss of activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. H4564
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
lower expression level
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
COMT plays a pivotal position in the lignin biosynthetic pathway
physiological function
-
TaCCoAOMT1 is an important CCoAOMT for lignin biosynthesis that is critical for stem development, but not directly associated with lodging-resistant trait in wheat
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FOMT2_WHEAT
356
0
38570
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38600
x * 38600, about, sequence calculation
28802
-
x * 28802, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38600, about, sequence calculation
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, expression of antisense enzyme in transgenic Nicotiana tabacum plants
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression in Escherichia coli
-
gene TaCCoAOMT1 is located in chromosome 7A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ma, Q.; Xu, Y.
Characterization of a caffeic acid 3-O-methyltransferase from wheat and its function in lignin biosynthesis
Biochimie
90
515-524
2008
Nicotiana tabacum (O24149), Nicotiana tabacum, Triticum aestivum (Q38J50), Triticum aestivum
Manually annotated by BRENDA team
Ma, Q.H.; Luo, H.R.
Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat
Planta
242
113-122
2015
Triticum aestivum
Manually annotated by BRENDA team
Ma, Q.; Luo, H.
Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat
Planta
242
113-122
2015
Triticum aestivum
Manually annotated by BRENDA team