We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The taxonomic range for the selected organisms is: Triticum aestivum The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
o-methyltransferase, ccoaomt, ccoaomt1, caffeic acid 3-o-methyltransferase, caffeoyl-coa o-methyltransferase, ccomt, ccoaomt2, ccoaomt-like, caffeoyl coa 3-o-methyltransferase, caffeoyl-coa 3-o-methyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
caffeic acid 3-O-methyltransferase
-
caffeoyl coenzyme A 3-O-methyltransferase
-
-
caffeoyl coenzyme A methyltransferase
-
-
-
-
caffeoyl-CoA 3-O-methyltransferase
-
-
-
-
trans-caffeoyl-CoA 3-O-methyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
methyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-, -, -, -, -, -, -, -, -
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine:caffeoyl-CoA 3-O-methyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxyconiferaldehyde
S-adenosyl-L-homocysteine + sinapaldehyde
-
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
-
-
-
?
S-adenosyl-L-methionine + caffeoyl alcohol
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + caffeoyl aldehyde
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
-
-
-
?
S-adenosyl-L-methionine + 4-coumaroyl-CoA
S-adenosyl-L-homocysteine + ?
-
very low activity
-
-
?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
S-adenosyl-L-methionine + 5-hydroxyferuoyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
S-adenosyl-L-methionine + feruloyl-CoA
S-adenosyl-L-homocysteine + ?
-
very low activity
-
-
?
S-adenosyl-L-methionine + sinapoyl-CoA
S-adenosyl-L-homocysteine + ?
-
low activity
-
-
?
additional information
?
-
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
-
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
-
56% activity compared to caffeoyl-CoA
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
-
best substrate
-
-
?
additional information
?
-
COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway
-
-
?
additional information
?
-
-
COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway
-
-
?
additional information
?
-
the enzyme is capable of 3'- and 5'-methylating not only acid, aldehyde and alcohol precursors, but also ester precursors, broad substrate specificity, overview
-
-
?
additional information
?
-
-
the enzyme is capable of 3'- and 5'-methylating not only acid, aldehyde and alcohol precursors, but also ester precursors, broad substrate specificity, overview
-
-
?
additional information
?
-
-
very poor substrates: 4-coumarate, caffeate, ferulate, 5-hydroxyferulate and sinapate, coumarylaldehyde, caffeoyaldehyde, coniferaldehyde, 5-hydroxyconiferaldehyde, sinapaldehyde, 4-coumaryl alcohol, caffeoyl alcohol, coniferyl alcohol, 5-hydroxyconiferyl alcohol and sinapyl alcohol
-
-
?
additional information
?
-
-
recombinant TaCCoAOMT1 protein can only use caffeoyl-CoA and 5-hydroxyferuloyl-CoA as effective substrates and caffeoyl-CoA as the best substrate. No activity with p-coumarate, caffeate, ferulate, 5-hydroxyferulate, sinapate, coumarylaldehyde, caffeoyaldehyde, coniferaldehyde, 5-hydroxyconiferaldehyde, and sinapaldehyde, 4-coumaryl alcohol, caffeoyl alcohol, coniferyl alcohol, 5-hydroxyconiferyl alcohol, and sinapyl alcohol
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
-
-
-
?
S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
S-adenosyl-L-homocysteine + sinapoyl-CoA
-
-
-
-
?
S-adenosyl-L-methionine + caffeoyl-CoA
S-adenosyl-L-homocysteine + feruloyl-CoA
-
-
-
-
?
additional information
?
-
additional information
?
-
COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway
-
-
?
additional information
?
-
-
COMT catalyzes the multi-step methylation reactions of hydroxylated monomeric lignin precursors, and plays a pivotal position in the lignin biosynthetic pathway
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine
-
S-adenosyl-L-methionine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0173
5-hydroxy-coniferaldehyde
-
0.0952
5-hydroxy-feruloyl-CoA
-
0.0437
caffeoyl aldehyde
-
0.00223
5-hydroxy-feruloyl-CoA
-
pH 7.5, 30°C, recombinant enzyme
0.0022
5-hydroxyferuoyl-CoA
-
pH 7.5, 30°C
0.112 - 0.1123
caffeoyl-CoA
0.112
caffeoyl-CoA
-
pH 7.5, 30°C
0.1123
caffeoyl-CoA
-
pH 7.5, 30°C, recombinant enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5.5
-
40% of maximum activity
6.5 - 9.5
-
the enzyme has a very narrow pH range, it is most active at pH 7.5, and inactive below pH 6.5 and above pH 9.5
8.5
-
40% of maximum activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
15 - 35
-
over 50% of maximal activity within this range, profile overview
40
-
40% of maximum activity
50
-
almost complete loss of activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.9
-
calculated
4.9
-
sequence calculation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
cv. H4564
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
lower expression level
brenda
-
high expression level
brenda
-
and stem, high expression
brenda
-
high expression level
brenda
-
and root, high expression
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
metabolism
COMT plays a pivotal position in the lignin biosynthetic pathway
physiological function
-
TaCCoAOMT1 is an important CCoAOMT for lignin biosynthesis that is critical for stem development, but not directly associated with lodging-resistant trait in wheat
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
FOMT2_WHEAT
356
0
38570
Swiss-Prot
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
38600
x * 38600, about, sequence calculation
28802
-
x * 28802, calculated
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 38600, about, sequence calculation
?
-
x * 28802, calculated
?
-
x * 28802, sequence calculation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum via Agrobacterium tumefaciens transfection, phenotype, overview
additional information
-
construction of transgenic COMT knockout Nicotiana tabacum plants by antisense expression of COMT from Triticum aestivum via Agrobacterium tumefaciens transfection, phenotype, overview
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, expression of antisense enzyme in transgenic Nicotiana tabacum plants
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression in Escherichia coli
-
gene TaCCoAOMT1 is located in chromosome 7A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, recombinant expression of the enzyme in Escherichia coli strain BL21(DE3)
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ma, Q.; Xu, Y.
Characterization of a caffeic acid 3-O-methyltransferase from wheat and its function in lignin biosynthesis
Biochimie
90
515-524
2008
Nicotiana tabacum (O24149), Nicotiana tabacum, Triticum aestivum (Q38J50), Triticum aestivum
brenda
Ma, Q.H.; Luo, H.R.
Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat
Planta
242
113-122
2015
Triticum aestivum
brenda
Ma, Q.; Luo, H.
Biochemical characterization of caffeoyl coenzyme A 3-O-methyltransferase from wheat
Planta
242
113-122
2015
Triticum aestivum
brenda