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Information on EC 1.97.1.9 - selenate reductase
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1.97.1.9 selenate reductaseIUBMB Comments
The periplasmic enzyme from Thauera selenatis is a complex comprising three heterologous subunits (alpha, beta and gamma) that contains molybdenum, iron, acid-labile sulfide and heme b as cofactor constituents. Nitrate, nitrite, chlorate and sulfate are not substrates. A number of compounds, including acetate, lactate, pyruvate, and certain sugars, amino acids, fatty acids, di- and tricarboxylic acids, and benzoate can serve as electron donors.
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Word Map
- 1.97.1.9
- nitrate
- selenium
- thauera
- molybdenum
- selenatis
- environmental protection
-
molybdoenzymes
- cloacae
-
twin-arginine
- ethylbenzene
-
alphabetagamma
-
molybdopterin-containing
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
selenate reductase, serabc, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
BisC
selenate reductase
serA
SrdA
SrdB
SrdC
YnfE
additional information
additional information
-
enzyme probably belongs to the DMSO reductase family of mononuclear molybdenum enzymes
additional information
-
enzyme probably belongs to the DMSO reductase family of mononuclear molybdenum enzymes
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
selenite + H2O + acceptor = selenate + reduced acceptor
active site molybdenum
-
selenite + H2O + acceptor = selenate + reduced acceptor
electron transport mechanism
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selenite + H2O + acceptor = selenate + reduced acceptor
selenate transport mechanism
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selenite + H2O + acceptor = selenate + reduced acceptor
A number of compounds, including acetate, lactate, pyruvate, and certain sugars, amino acids, fatty acids, di- and tricarboxylic acids, and benzoate can serve as electron donors
-
-
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selenite + H2O + acceptor = selenate + reduced acceptor
active site molybdenum
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
selenite:reduced acceptor oxidoreductase
The periplasmic enzyme from Thauera selenatis is a complex comprising three heterologous subunits (alpha, beta and gamma) that contains molybdenum, iron, acid-labile sulfide and heme b as cofactor constituents. Nitrate, nitrite, chlorate and sulfate are not substrates. A number of compounds, including acetate, lactate, pyruvate, and certain sugars, amino acids, fatty acids, di- and tricarboxylic acids, and benzoate can serve as electron donors.
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CAS REGISTRY NUMBER
COMMENTARY
146359-71-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
selenate + reduced cytc-Ts4
selenite + H2O + oxidized cytc-Ts4
-
a c-type cytochrom is purified and shown to donate electrons to SerABC in vitro. Redox potentiometry, combined with UV-visible spectroscopy, show that cytc-Ts4 is a diheme cytochrome with a redox potential of +/-282 mV, and both hemes are predicted to have His-Met ligation
-
-
?
selenate + reduced methyl viologen
selenite + H2O + methyl viologen
-
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized donor
Enterobacter cloacae SLD1a-1 / ATCC 700258
-
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
Enterobacter cloacae SLD1a-1 / ATCC 700258
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
enzyme also reduces nitrate, thiosulfate, and fumarate
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
enzyme also reduces nitrate, thiosulfate, and fumarate
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
specific for selenate
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
specific for selenate
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + lactate
selenite + H2O + acetate + HCO3-
-
-
-
?
selenate + lactate
selenite + H2O + acetate + HCO3-
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-
-
?
selenate + lactate
selenite + H2O + acetate + HCO3-
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
detoxification of selenate
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
Enterobacter cloacae SLD1a-1 / ATCC 700258
-
detoxification of selenate
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
Enterobacter cloacae SLD1a-1 / ATCC 700258
-
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
?
selenate + reduced benzyl viologen
selenite + H2O + benzyl viologen
-
-
-
-
?
selenate + reduced benzyl viologen
selenite + H2O + benzyl viologen
Enterobacter cloacae SLD1a-1 / ATCC 700258
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-
-
-
?
selenate + reduced benzyl viologen
selenite + H2O + oxidized benzyl viologen
-
-
-
-
?
selenate + reduced benzyl viologen
selenite + H2O + oxidized benzyl viologen
Enterobacter cloacae SLD1a-1 / ATCC 700258
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-
-
-
?
selenate + reduced benzyl viologen
selenite + H2O + oxidized benzyl viologen
-
-
-
-
?
selenate + reduced benzyl viologen
selenite + H2O + oxidized benzyl viologen
-
best electron donor
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-
?
selenate + reduced methyl viologen
selenite + H2O + oxidized methyl viologen
-
preferred electron donor
-
-
?
selenate + reduced methyl viologen
selenite + H2O + oxidized methyl viologen
Enterobacter cloacae SLD1a-1 / ATCC 700258
-
preferred electron donor
-
-
?
selenate + reduced methyl viologen
selenite + H2O + oxidized methyl viologen
-
-
-
-
?
selenate + reduced methyl viologen
selenite + H2O + oxidized methyl viologen
-
-
-
-
?
selenate + reduced methyl viologen
selenite + H2O + oxidized methyl viologen
-
-
-
-
?
selenate + reduced methyl viologen
selenite + H2O + oxidized methyl viologen
-
11% of the activity with benzyl viologen
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-
?
additional information
?
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-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
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-
?
additional information
?
-
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no substrate: nitrate, sulfate, perchlorate, thiosulfate
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-
?
additional information
?
-
Enterobacter cloacae SLD1a-1 / ATCC 700258
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no substrate: nitrate, sulfate, perchlorate, thiosulfate
-
-
?
additional information
?
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the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
Gottschalkia acidurici ATCC 7906
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
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?
additional information
?
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also active on nitrate, thiosulfate and fumarate
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?
additional information
?
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NADH, succinate, and lactate are no electron donors
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-
?
additional information
?
-
-
no activity with nitrate, nitrite, sulfate, and chlorate
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-
?
additional information
?
-
-
no activity with nitrate, nitrite, sulfate, and chlorate
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
-
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + electron donor
selenite + H2O + oxidized electron donor
selenate is the terminal electron acceptor in dissimilatory selenate reduction
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
detoxification of selenate
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
Enterobacter cloacae SLD1a-1 / ATCC 700258
-
detoxification of selenate
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
?
selenate + reduced acceptor
selenite + H2O + acceptor
-
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
Gottschalkia acidurici ATCC 7906
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
additional information
?
-
-
the organism is part of an enrichment of a bacterial assemblage from a mine impacted natural marsh sediment that is capable of simultaneous selenate reduction and denitrification, selenate reduction in selenate enrichment culture, SeRB, and selenate reduction in the presence of varying concentrations of nitrate, overview
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
iron-sulfur center
-
activity of genes iscU or hscB in the Isc iron-sulfur cluster biosynthesis pathway is required for formation of selenate reductase activity in vivo
iron-sulfur centre
-
at least 2 [Fe-S]-centre as prosthetic groups per enzyme molecule
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Iron
Molybdenum
selenium
-
isolated enzyme contains a reduced form of selenium, probably as selenocysteine
Iron
-
part of at least 2 iron-sulfur centres per enzyme molecule and of the heme group of cytochrome b
Molybdenum
-
enhances the activity 10fold in vivo when added to the growth medium
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
EDTA
-
inhibits the enzymatic reducing activity slightly, addition of Mo2+ and Zn2+ to the reaction mixture eliminates the EDTA inhibitory effect
tungstate
-
SER isolated from periplasmic fractions from cells grown on 1 mM tungstate display selenate reductase activities with a 20fold reduction in Vmax and a 23fold increase in substrate binding affinity. The thermo-stability and pH dependence of tungsten-SER is shown to be similar to that observed for molybdenum-SER
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0007
selenate
-
tungsten-SER, pH and temperature not specified in the publication, Vmax: 0.01 micromol/min/mg