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Information on EC 1.97.1.12 - photosystem I and Organism(s) Synechocystis sp. and UniProt Accession P29255

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IUBMB Comments
Contains chlorophyll, phylloquinones, carotenoids and [4Fe-4S] clusters. Cytochrome c6 can act as an alternative electron donor, and flavodoxin as an alternative acceptor in some species.
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Synechocystis sp.
UNIPROT: P29255 not found.
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The taxonomic range for the selected organisms is: Synechocystis sp.
The enzyme appears in selected viruses and cellular organisms
Synonyms
photosystem i, psi core complex, ps-i complex, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
plastocyanin:ferredoxin oxidoreductase (light-dependent)
Contains chlorophyll, phylloquinones, carotenoids and [4Fe-4S] clusters. Cytochrome c6 can act as an alternative electron donor, and flavodoxin as an alternative acceptor in some species.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
reduced plastocyanin + oxidized ferredoxin + hv
oxidized plastocyanin + reduced ferredoxin
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
reduced plastocyanin + oxidized ferredoxin + hv
oxidized plastocyanin + reduced ferredoxin
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-carotene
-
cyanobacterial PSI complexes contain 22 molecules of beta-carotene, 17 of which are in all-trans configuration
chlorophyll a
-
most abundant cofactor in PSI, role of these molecules in light absorption, charge separation, electron transfer, and biogenesis
chlorophyll a'
-
one member of the P700 special pair is a chlorophyll a' molecule
iron-sulfur centre
-
a PSI complex contains 12 iron atoms that constitute 3 [4Fe-4S] clusters
Lipid
-
four lipid molecules can be assigned in the high-resolution structure of PSI. Three of these molecules are phosphatidylglycerol and one is monogalactosyldiacylglycerol. These molecules are embedded in the PSI complex, with the acyl chains anchored among transmembrane helices. The phosphodiester group of one of the phospholipids coordinates an antenna chlorophyll molecule
phylloquinone
-
the PSI complex of cyanobacteria and chloroplasts contains two phylloquinone molecules, which function in the electron transfer as the redox center A1
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007
reduced plastocyanin
-
pH and temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
a cyanobacterial PSI monomer consists of 11–12 protein subunits
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F647C
-
mutant strain cannot grow under photoautotrophic conditions because of low photosystem I activity, possibly due to low levels of proteins
F649C/G650I
-
mutant strain cannot grow under photoautotrophic conditions because of low photosystem I activity, possibly due to low levels of proteins
H651C/L652M
-
mutant strain cannot grow under photoautotrophic conditions because of low photosystem I activity, possibly due to low levels of proteins
S641C/V642I
-
mutation in subunit PsaB. Mutant strain grows photoautotrophically and shows no obvious reduction in the photosystem I activity. Kinetics of P700 re-reduction by plastocyanin remains unaltered
W643C/A644I
-
mutation in subunit PsaB. Mutant strain grows photoautotrophically and shows no obvious reduction in the photosystem I activity. Kinetics of P700 re-reduction by plastocyanin remains unaltered
W645C
-
mutation in subunit PsaB. Mutant strain grows photoautotrophically and shows no obvious reduction in the photosystem I activity. Kinetics of P700 re-reduction by plastocyanin remains unaltered
additional information
-
molecular analysis of spontaneous revertants from the mutants H651C/L652M, F649C/G650I and F647C suggests that an aromatic residue at F647 and a small residue at G650 may be necessary for maintaining the structural integrity of photosystem I
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chitnis, P.R.
Photosystem I: function and physiology
Annu. Rev. Plant Physiol. Plant Mol. Biol.
52
593-626
2001
Synechocystis sp., Arabidopsis thaliana, Chlamydomonas reinhardtii
Manually annotated by BRENDA team
van Thor, J.J.; Geerlings, T.H.; Matthijs, H.C.; Hellingwerf, K.J.
Kinetic evidence for the PsaE-dependent transient ternary complex photosystem I/ferredoxin/ferredoxin:NADP+ reductase in a cyanobacterium
Biochemistry
38
12735-12746
1999
Synechocystis sp.
Manually annotated by BRENDA team
Kubota, H.; Sakurai, I.; Katayama, K.; Mizusawa, N.; Ohashi, S.; Kobayashi, M.; Zhang, P.; Aro, E.M.; Wada, H.
Purification and characterization of photosystem I complex from Synechocystis sp. PCC 6803 by expressing histidine-tagged subunits
Biochim. Biophys. Acta
1797
98-105
2010
Synechocystis sp.
Manually annotated by BRENDA team
Xu, W.; Wang, Y.; Taylor, E.; Laujac, A.; Gao, L.; Savikhin, S.; Chitnis, P.
Mutational analysis of photosystem I of Synechocystis sp. PCC 6803: The role of four conserved aromatic residues in the j-helix of PsaB
PLoS ONE
6
e24625
2011
Synechocystis sp.
Manually annotated by BRENDA team