Information on EC 1.8.99.B3 - Cys-type anaerobic sulfatase-maturating enzyme

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.8.99.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Cys-type anaerobic sulfatase-maturating enzyme
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a [sulfatase]-L-cysteine + S-adenosyl-L-methionine + reduced acceptor = a [sulfatase]-3-oxo-L-alanine + L-methionine + 5'-deoxyadenosine + acceptor
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
[sulfatase]-L-cysteine:S-adenosyl-L-methionine oxidoreductase (3-oxo-L-alanine-forming)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ac-AVPSCIPSRASILTGM-NH2 + S-adenosyl-L-methionine + dithionite
Ac-AVPS-3-oxo-L-Ala-IPSRASILTGM-NH2+ L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
show the reaction diagram
Ac-FENAYTAVPSCIASRASILTGMS-NH2 + S-adenosyl-L-methionine + reduced acceptor
Ac-FENAYTAVPS-3-oxo-L-Ala-IASRASILTGMS-NH2 + L-methionine + 5'-deoxyadenosine + acceptor + hydrogen sulfide
show the reaction diagram
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?
Ac-FENAYTAVPSCIASRASILTMSQ-NH2 + S-adenosyl-L-methionine + dithionite
Ac-FENAYTAVPS-3-oxo-L-Ala-IASRASILTMSQ-NH2 + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
show the reaction diagram
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?
Ac-YTAVPSCIPSRASILTGM + S-adenosyl-L-methionine + dithionite
Ac-YTAVPS-3-oxo-L-Ala-IPSRASILTGM + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
show the reaction diagram
Ac-YYTSPMCAPARSMLLTGN + S-adenosyl-L-methionine
Ac-YYTSPM-3-oxo-L-Ala-APARSMLLTGN + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
show the reaction diagram
Ac-YYTSPMSAPARSMLLTGN + S-adenosyl-L-methionine
Ac-YYTSPM3-oxo-L-Ala-APARSMLLTGN + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
show the reaction diagram
Ac-YYTSPMSeCAPARSMLLTGN + S-adenosyl-L-methionine
Ac-YYTSPM-3-oxo-L-Ala-APARSMLLTGN + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen selenide
show the reaction diagram
Ac-YYTSPMTAPARSMLLTGN + S-adenosyl-L-methionine
Ac-YYTSPM-(2S)-2-amino-3-oxobutanoyl-APARSMLLTGN + L-methionine + 5'-deoxyadenosine + sulfur dioxide + H2O
show the reaction diagram
a flavodoxin/flavodoxin reductase/NADPH system may substitute for dithionite
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?
Tyr-Tyr-Thr-Ser-Pro-Met-Cys-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + S-adenosyl-L-methionine
Tyr-Tyr-Thr-Ser-Pro-Met-3-oxo-L-Ala-Ala-Pro-Ala-Arg-Ser-Met-Leu-Leu-Thr-Gly-Asn + L-methionine + 5'-deoxyadenosine + sulfur dioxide + hydrogen sulfide
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
[4Fe-4S]2+ cluster
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45740
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure at 1.6-1.8 A resolution
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 0.1 M Tris-HCl buffer, pH 7, 150 mM KCl and 5 mM DTT, anaerobic conditions
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-80°C, 50 mM potassium HEPES, pH 7.5, 500 mM KCl, 10 mM DTT, and 30% glycerol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal affinity chromatography under an atmosphere of nitrogen
Ni-NTA Sepharose and Superdex S200HR chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli BL21(DE3)
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C15A/C19A/C22A