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EC Tree
IUBMB Comments Uses adenylyl sulfate, not phosphoadenylyl sulfate, distinguishing this enzyme from EC 1.8.4.8, phosphoadenylyl-sulfate reductase (thioredoxin). Uses thioredoxin as electron donor, not glutathione or other donors, distinguishing it from EC 1.8.4.9 [adenylyl-sulfate reductase (glutathione)] and EC 1.8.99.2 (adenylyl-sulfate reductase).
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
paapr, apr-b, thioredoxin-dependent aps reductase,
more
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5'-adenylylsulfate reductase
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thioredoxin dependent 5'-adenylylsulfate reductase
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thioredoxin-dependent APS reductase
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-
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AMP,sulfite:thioredoxin-disulfide oxidoreductase (adenosine-5'-phosphosulfate-forming)
Uses adenylyl sulfate, not phosphoadenylyl sulfate, distinguishing this enzyme from EC 1.8.4.8, phosphoadenylyl-sulfate reductase (thioredoxin). Uses thioredoxin as electron donor, not glutathione or other donors, distinguishing it from EC 1.8.4.9 [adenylyl-sulfate reductase (glutathione)] and EC 1.8.99.2 (adenylyl-sulfate reductase).
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5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
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-
-
?
adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
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-
-
-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
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?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
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-
?
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5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
no activity with 3'-phosphoadenosine-5'-phosphosulfate, i.e. PAPS
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-
?
5'-adenylyl sulfate + thioredoxin
AMP + sulfite + thioredoxin disulfide
involved in assimilatory sulfate reduction
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-
?
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thioredoxin
5fold lower activity with dithiothreithol, 70fold lower activity with E. coli Grx1 or dithionite, 580fold lower activity with lipoic acid, no activity with ferredoxin
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Fe-S cluster
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[4Fe-4S] cluster
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5'-AMP
competitive vs. adenosine-5'-phosphosulfate
adenosine-5'-phosphosulfate
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additional information
not activated by sodium sulfate
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0.00175
adenosine-5'-phosphosulfate
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0.0114
5'-adenylyl sulfate
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0.01
thioredoxin
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wild type enzyme, at 20°C in 10 mM phosphate buffer (pH 7.5) containing 100 mM Na2SO4
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1
adenosine-5'-phosphosulfate
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SwissProt
brenda
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C140S
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mutant enzyme shows 3% of wild-type activity
additional information
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a heterologous system is constructed in which the C domain of EiAPR (EC 1.8.4.9) is fused to the carboxyl terminus of the APS reductase from Pseudomonas aeruginosa (EC 1.8.4.10), an enzyme that normally uses thioredoxin as an electron donor and is incapable of using glutathione for this function. The hybrid enzyme, which retains the [4Fe-4S] cluster from PaAPR, can use both thioredoxin and glutathione as an electron donor for APS reduction. The ability to use glutathione is enhanced by the addition of Na2SO4 to the reaction buffer, a property that the hybrid enzyme shares with EiAPR
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5.7
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the enzyme is unstable at 30°C in 20 mM phosphate buffer (pH 5.7)
698950
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the enzyme is unstable at 30°C in 20 mM phosphate buffer (pH 5.7)
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expression in transgenic Zea mays
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Bick, J.A.; Dennis, J.J.; Zylstra, G.J.; Nowack, J.; Leustek, T.
Identification of a new class of 5'-adenylylsulfate (APS) reductases from sulfate-assimilating bacteria
J. Bacteriol.
182
135-142
2000
Burkholderia cepacia (Q9RFS6), Burkholderia cepacia DBO1 / ATCC 29424 (Q9RFS6), Mycobacterium sp., Pseudomonas aeruginosa (O05927), Pseudomonas putida, Ralstonia pickettii, Rhizobium tropici
brenda
Kim, S.K.; Gomes, V.; Gao, Y.; Chandramouli, K.; Johnson, M.K.; Knaff, D.B.; Leustek, T.
The two-domain structure of 5-adenylylsulfate (APS) reductase from Enteromorpha intestinalis is a requirement for efficient APS reductase activity
Biochemistry
46
591-601
2007
Pseudomonas aeruginosa
brenda
Kim, S.; Rahman, A.; Mason, J.T.; Hirasawa, M.; Conover, R.C.; Johnson, M.K.; Miginiac-Maslow, M.; Keryer, E.; Knaff, D.B.; Leustek, T.
The interaction of 5-adenylylsulfate reductase from Pseudomonas aeruginosa with its substrates
Biochim. Biophys. Acta
1710
103-112
2005
Pseudomonas aeruginosa
brenda
Martin, M.N.; Tarczynski, M.C.; Shen, B.; Leustek, T.
The role of 5-adenylylsulfate reductase in controlling sulfate reduction in plants
Photosynth. Res.
86
309-323
2005
Pseudomonas aeruginosa
brenda
Chung, J.S.; Noguera-Mazon, V.; Lancelin, J.M.; Kim, S.K.; Hirasawa, M.; Hologne, M.; Leustek, T.; Knaff, D.B.
Interaction domain on thioredoxin for Pseudomonas aeruginosa 5'-adenylylsulfate reductase
J. Biol. Chem.
284
31181-31189
2009
Pseudomonas aeruginosa
brenda