Information on EC 1.8.4.1 - glutathione-homocystine transhydrogenase

for references in articles please use BRENDA:EC1.8.4.1
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The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
1.8.4.1
-
top print hide
RECOMMENDED NAME
GeneOntology No.
glutathione-homocystine transhydrogenase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 glutathione + homocystine = glutathione disulfide + 2 homocysteine
show the reaction diagram
the reactions catalyzed by this enzyme and by other in this subclass may be similar to those catalyzed by EC 2.5.1.18
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
-
-
Glutathione metabolism
-
-
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SYSTEMATIC NAME
IUBMB Comments
glutathione:homocystine oxidoreductase
The reactions catalysed by this enzyme and by others in this subclass may be similar to those catalysed by EC 2.5.1.18 glutathione transferase.
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top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
9029-40-7
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 glutathione + homocystine
glutathione disulfide + 2 homocysteine
show the reaction diagram
-
-
-
-
?
2 lysine + homocystine
glutathione disulfide + 2 homocysteine
show the reaction diagram
-
-
-
-
?
cysteine + homocystine
cystine + 2 homocysteine
show the reaction diagram
-
-
-
-
?
GSH + homocystine
GSSG + homocysteine
show the reaction diagram
-
-
-
r
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GSH + homocystine
GSSG + homocysteine
show the reaction diagram
-
-
-
r
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
15.1
cysteine
-
pH 5.2, 40°C
14.3
glutathione
-
pH 5.2, 40°C
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.056
cysteine
-
pH 5.2, 40°C
0.078
glutathione
-
pH 5.2, 40°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
289.9
-
pH 5.2, 40°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
79000 - 80000
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 35000, SDS-PAGE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
-
743409
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
top print hide
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
maximum activity is obtained by nutritional optimization using GSH (0.4%) combined with homocystine (0.01%) and glucose (0.4%), NADH (30 mM) at medium initial pH 7.8. The yield of GHTHase is increased about 1.2 times upon starvation of the culture for two days, as compared to the non-sulfur starved culture. The GHTHase activity is increased by 13.7fold
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LINKS TO OTHER DATABASES (specific for EC-Number 1.8.4.1)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)