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Information on EC 1.8.3.2 - thiol oxidase and Organism(s) Cavia porcellus and UniProt Accession O08841

for references in articles please use BRENDA:EC1.8.3.2
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EC Tree
     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.3 With oxygen as acceptor
                1.8.3.2 thiol oxidase
IUBMB Comments
R may be =S or =O, or a variety of other groups. The enzyme is not specific for R'.
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This record set is specific for:
Cavia porcellus
UNIPROT: O08841
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Word Map
The taxonomic range for the selected organisms is: Cavia porcellus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
qsox1, sulfhydryl oxidase, augmenter of liver regeneration, ero1p, thiol oxidase, hepatopoietin, erv2p, erv1p, sulphydryl oxidase, sfalr, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sulfhydryl oxidase
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DTT-oxidase
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-
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ERv2p
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oxidase, thiol
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-
-
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Quiescin-sulfhydryl oxidase
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sulfhydryl oxidase
sulfhydryl oxidase SOx-3
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-
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thiooxidase
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-
-
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
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oxidation
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-
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reduction
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-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
thiol:oxygen oxidoreductase
R may be =S or =O, or a variety of other groups. The enzyme is not specific for R'.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-39-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dithiothreitol + O2
dithiothreitol disulfide + H2O2
show the reaction diagram
-
-
-
ir
R-SH + O2
R-S-S-R + H2O2
show the reaction diagram
R-SH + O2
R-S-S-R + H2O2
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
R-SH + O2
R-S-S-R + H2O2
show the reaction diagram
enzyme plays a role in synaptic strengthening and in redox activities in the brain
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ir
R-SH + O2
R-S-S-R + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
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small FAD-binding domain
additional information
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enzyme contains a thioredoxin domain
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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enzyme expression in endometrial glandular epithelium is inducible by estradiol in presence of cycloheximide, and by serum deprivation
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
detailed tissue distribution analysis in brain, overview
Manually annotated by BRENDA team
highest enzyme expression in reticular structures, such as the basal forebrain, reticular thalamic nucleus, and reticular nuclei of the brainstem
Manually annotated by BRENDA team
endometrial cells. Enzyme level increases during a serum depletion-induced quiescence, decreases when cells enter the g1 phase after serum stimulation, and is restored during the S and G2/M phases
Manually annotated by BRENDA team
specific expression in glandular and luminal epithelial cells
Manually annotated by BRENDA team
secretory, endometrial
Manually annotated by BRENDA team
epithelial cells
Manually annotated by BRENDA team
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glandular epithelial cells
Manually annotated by BRENDA team
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endometrial glandular
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
enzyme is secreted
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Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
QSOX1_CAVPO
613
1
68595
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
x * 68000, recombinant His-tagged enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 68000, recombinant His-tagged enzyme, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel chelate chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene cpQSOx1, primary gene sequence, DNA and amino acid sequence analysis, stable expression of the wild-type enzyme in MCF-7 cells with intra- and extracellular localization of the recombinant enzyme, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
overexpression in MCF-7 cell lines
DNA and amino acid sequence determination and analysis, gene structure
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
protective role of QSOX1 against apoptosis. QSOX1 short transcript overexpression slows down proliferation and protects cells from oxidative stress-induced cell death
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Musard, J.F.; Sallot, M.; Dulieu, P.; Fraichard, A.; Ordener, C.; Remy-Martin, J.P.; Jouvenot, M.; Adami, P.
Identification and expression of a new sulfhydryl oxidase SOx-3 during the cell cycle and the estrus cycle in uterine cells
Biochem. Biophys. Res. Commun.
287
83-91
2001
Cavia porcellus (O08841), Cavia porcellus
Manually annotated by BRENDA team
Thorpe, C.; Hoober, K.L.; Raje, S.; Glynn, N.M.; Burnside, J.; Turi, G.K.; Coppock, D.L.
Sulfhydryl oxidases: emerging catalysts of protein disulfide bond formation in eukaryotes
Arch. Biochem. Biophys.
405
1-12
2002
Aspergillus niger, Cavia porcellus, Gallus gallus, Homo sapiens (O00391), Homo sapiens, Rattus norvegicus (Q6IUU3), Saccharomyces cerevisiae, Trypanosoma brucei
Manually annotated by BRENDA team
Amiot, C.; Musard, J.F.; Hadjiyiassemis, M.; Jouvenot, M.; Fellmann, D.; Risold, P.Y.; Adami, P.
Expression of the secreted FAD-dependent sulfhydryl oxidase (QSOX) in the guinea pig central nervous system
Mol. Brain Res.
125
13-21
2004
Cavia porcellus (O08841)
Manually annotated by BRENDA team
Morel, C.; Adami, P.; Musard, J.F.; Duval, D.; Radom, J.; Jouvenot, M.
Involvement of sulfhydryl oxidase QSOX1 in the protection of cells against oxidative stress-induced apoptosis
Exp. Cell Res.
313
3971-3982
2007
Rattus norvegicus, Cavia porcellus (O08841), Cavia porcellus
Manually annotated by BRENDA team