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Information on EC 1.8.3.1 - sulfite oxidase and Organism(s) Gallus gallus and UniProt Accession P07850
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1.8.3.1 sulfite oxidaseIUBMB Comments
A molybdohemoprotein.
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Word Map
- 1.8.3.1
- molybdenum
- sulfur
- xanthine
- heme
- thiosulfate
- moco
- epr
- seizures
- molybdopterin
-
molybdoenzymes
-
sulfur-containing
- tungsten
-
molybdenum-containing
- pterin
- s-sulfocysteine
- soxs
- sulfurtransferase
-
ectopia
-
low-ph
-
pyranopterins
- lentis
-
dithiolene
-
amidoxime
-
eseem
-
hyperfine
- food industry
- agriculture
-
high-ph
- analysis
-
oxidase-deficient
- medicine
-
xanthinuria
-
marc
- molecular biology
- encephalomalacia
The taxonomic range for the selected organisms is: Gallus gallus
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
sulfite oxidase, shopper, atsox, sulfite:acceptor oxidoreductase, at-so, yedyz, sulfite oxidase homologue, sulfite acceptor oxidoreductase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
oxidase, sulfite
-
-
-
-
sulphite oxidase cytochrome b9
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sulfite + O2 + H2O = sulfate + H2O2
x-ray absorption spectroscopy of oxidation states
-
sulfite + O2 + H2O = sulfate + H2O2
intramolecular electron transfer and effect of solution viscosity
-
sulfite + O2 + H2O = sulfate + H2O2
mechanism and kinetics of electron transfer
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -
SYSTEMATIC NAME
IUBMB Comments
sulfite:oxygen oxidoreductase
A molybdohemoprotein.
CAS REGISTRY NUMBER
COMMENTARY
9029-38-3
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
sulfite + H2O + A
SO42- + AH2
-
A: electron acceptor, i.e. O2, cytochrome c, K3[Fe(CN)6], 2,6-dichloroindophenol, methylene blue, highly specific for sulfite as electron donor
-
-
?
additional information
?
-
R138, R190, and R450 contribute to a positively charged binding pocket, which stabilizes substrate/product binding
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
molybdenum cofactor
the enzyme contains one molybdenum active site per subunit
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mo
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
K2HPO4
-
at 26 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
K2SO4
-
at 22 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
KCl
-
at 95 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
KF
-
at 72 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
KNCS
-
at 57 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
KNO3
-
at 78 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
N-cyclohexyl-N'-[2-(N-methylmorpholino)-ethyl]carbodiimide p-toluene sulfonate
-
CMC
NaCl
-
at 100 mM 50% inhibition if cytochrome c or ferricyanide is electron acceptor
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). SUOX_CHICK
459
0
50205
Swiss-Prot
other Location (Reliability: 5)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55000
-
? * 55000, SDS-PAGE, heterogenous behaviour in sedimentation equilibrium experiments
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
? * 55000, SDS-PAGE, heterogenous behaviour in sedimentation equilibrium experiments
dimer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
R138Q
the side chain nitrogen of the Gln appears to be within the coordination sphere of the Mo
Y322F/R450M
introduction of predicted catalytic site residues of assimilatory nitrate reductase, markedly decreased ability to bind sulfite at pH 8.5
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
-
electroimmobilisation into polypyrrole film, use for amperometric detection of sulfite
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Neame, P.J.; Barber, M.J.
Conserved domains in molybdenum hydroxylases. The amino acid sequence of chicken hepatic sulfite oxidase
J. Biol. Chem.
264
20894-20901
1989
Gallus gallus
Kipke, C.A.; Enemark, J.H.; Sunde, R.A.
Purification of prosthetically intact sulfite oxidase from chicken liver using a modified procedure
Arch. Biochem. Biophys.
270
383-390
1989
Gallus gallus
Ritzman, M.; Bosshard, H.R.
Sulfite oxidase from chicken liver. Further characterization of the role of carboxyl groups in the reaction with cytochrome c
Eur. J. Biochem.
172
377-381
1988
Gallus gallus
Ritzmann, M.; Bosshard, H.R.
Sulfite oxidase from chicken liver. The role of imidazole and carboxyl groups for the reaction with cytochrome c
Eur. J. Biochem.
159
493-497
1986
Gallus gallus
Kramer, S.P.; Johnson, J.L.; Ribeiro, A.A.; Millington, D.S.; Rajagopalan, K.V.
The structure of the molybdenum cofactor. Characterization of di-(carboxamidomethyl)molybdopterin from sulfite oxidase and xanthine oxidase
J. Biol. Chem.
262
16357-16363
1987
Gallus gallus
Johnson, J.L.; Hainline, B.E.; Rajagopalan, K.V.
Characterization of the molybdenum cofactor of sulfite oxidase, xanthine, oxidase, and nitrate reductase. Identification of a pteridine as a structural component
J. Biol. Chem.
255
1783-1786
1980
Gallus gallus, Rattus norvegicus
Bray, R.C.
Molybdenum iron-sulfur flavin hydroxylases and related enzymes
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
12
299-419
1975
Bos taurus, Gallus gallus, Oryctolagus cuniculus, Rattus norvegicus, Sus scrofa
-
Kessler, D.L.; Rajagopalan, K.V.
Hepatic sulfite oxidase. Identification of the molybdenum center as the site of irreversible inactivation by ferricyanide
Biochim. Biophys. Acta
370
399-409
1974
Gallus gallus, Rattus norvegicus
Kessler, D.L.; Rajagopalan, K.V.
Hepatic sulfite oxidase. Effect of anions on interaction with cytochrome c
Biochim. Biophys. Acta
370
389-398
1974
Gallus gallus
Kessler, D.L.; Rajagopalan, K.V.
Purification and properties of sulfite oxidase from chicken liver. Presence of molybdenum in sulfite oxidase from diverse sources
J. Biol. Chem.
247
6566-6573
1972
Gallus gallus
George, G.N.; Kipke, C.A.; Prince, R.C.; Sunde, R.A.; Enemark, J.H.; Cramer, S.P.
Structure of the active site of sulfite oxidase. X-ray absorption spectroscopy of the Mo(IV), Mo(V), and Mo(VI) oxidation states
Biochemistry
28
5075-5080
1989
Gallus gallus
Kipke, C.A.; Cusanovich, M.A.; Tolin, G.; Sunde, R.A.; Enemark, J.H.
Chicken liver sulfite oxidase. Kinetics of reduction by laser-photoreduced flavins and intramolecular electron transfer
Biochemistry
27
2918-2926
1988
Gallus gallus
Sullivan, E.P., Jr.; Hazzard, J.T.; Tollin, G.; Enemark, J.H.
Electron transfer in sulfite oxidase: Effects of pH and anions on transient kinetics
Biochemistry
32
12465-12470
1993
Gallus gallus
Ratnam, K.; Brody, M.S.; Hille, R.
Purification of xanthine dehydrogenase and sulfite oxidase from chicken liver
Prep. Biochem. Biotechnol.
26
143-154
1996
Gallus gallus
Feng, C.; Kedia, R.V.; Hazzard, J.T.; Hurley, J.K.; Tollin, G.; Enemark, J.H.
Effect of solution viscosity on intramolecular electron transfer in sulfite oxidase
Biochemistry
41
5816-5821
2002
Gallus gallus
Brody, M.S.; Hille, R.
The kinetic behavior of chicken liver sulfite oxidase
Biochemistry
38
6668-6677
1999
Gallus gallus
Pacheco, A.; Hazzard, J.T.; Tollin, G.; Enemark, J.H.
The pH dependence of intramolecular electron transfer rates in sulfite oxidase at high and low anion concentrations
J. Biol. Inorg. Chem.
4
390-401
1999
Gallus gallus
Das, S.K.; Chaudhury, P.K.; Biswas, D.; Sarkar, S.
Modeling for the Active Site of Sulfite Oxidase: Synthesis, Characterization, and Reactivity of [MoVIO2(mnt)2]2- (mnt2- = 1,2-Dicyanoethylenedithiolate)
J. Am. Chem. Soc.
116
9061-9070
1994
Gallus gallus
-
Adeloju, S.B.; Barisci, J.N.; Wallace, G.G.
Electroimmobilization of sulfite oxidase into a polypyrrole film and its utilization for flow amperometric detection of sulfite
Anal. Chim. Acta
332
145-153
1996
Gallus gallus
-
Workun, G.J.; Moquin, K.; Rothery, R.A.; Weiner, J.H.
Evolutionary persistence of the molybdopyranopterin-containing sulfite oxidase protein fold
Microbiol. Mol. Biol. Rev.
72
228-48
2008
Arabidopsis thaliana (Q9S850), Bacillus licheniformis, Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) NRRL B-14911, Chlorella vulgaris, Deinococcus radiodurans, Erythrobacter litoralis, Escherichia coli, Gallus gallus (P07850), Homo sapiens, Maribacter sp. HTCC2170, Ogataea angusta, Paenarthrobacter aurescens, Rhizorhabdus wittichii RW1 (A5V4U9), Roseovarius nubinhibens, Rubrobacter xylanophilus, Saccharopolyspora erythraea, Spinacia oleracea, Streptomyces ambofaciens, Thermus thermophilus, Zea mays
Haensch, R.; Lang, C.; Rennenberg, H.; Mendel, R.R.
Significance of plant sulfite oxidase
Plant Biol.
9
589-595
2007
Arabidopsis thaliana, Cytisus scoparius, Gallus gallus, Hedera helix, Homo sapiens, Nicotiana plumbaginifolia, Phragmites australis, Populus tremula x Populus alba, Quercus ilex, Rattus norvegicus, Spinacia oleracea
Klein, E.; Astashkin, A.; Ganyushin, D.; Riplinger, C.; Johnson-Winters, K.; Neese, F.; Enemark, J.
Direct detection and characterization of chloride in the active site of the low-pH form of sulfite oxidase using electron spin echo envelope modulation spectroscopy, isotopic labeling, and density functional theory calculations
Inorg. Chem.
48
4743-4752
2009
Gallus gallus (P07850)
Astashkin, A.V.; Klein, E.L.; Ganyushin, D.; Johnson-Winters, K.; Neese, F.; Kappler, U.; Enemark, J.H.
Exchangeable oxygens in the vicinity of the molybdenum center of the high-pH form of sulfite oxidase and sulfite dehydrogenase
Phys. Chem. Chem. Phys.
11
6733-6742
2009
Gallus gallus (P07850)
Qiu, J.; Wilson, H.; Rajagopalan, K.
Structure-based alteration of substrate specificity and catalytic activity of sulfite oxidase from sulfite oxidation to nitrate reduction
Biochemistry
51
1134-1147
2012
Gallus gallus (P07850), Gallus gallus, Homo sapiens
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