Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.8.1.7 - glutathione-disulfide reductase and Organism(s) Schistosoma mansoni and UniProt Accession Q962Y6

for references in articles please use BRENDA:EC1.8.1.7
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
A dimeric flavoprotein (FAD); activity is dependent on a redox-active disulfide in each of the active centres.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Schistosoma mansoni
UNIPROT: Q962Y6
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Schistosoma mansoni
The enzyme appears in selected viruses and cellular organisms
Synonyms
glutathione reductase, gsh reductase, gssg reductase, glutathione disulfide reductase, ptgr2, gsr-1, glutathione-disulfide reductase, glutathione s-reductase, thioredoxin/glutathione reductase, trxr3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
thioredoxin glutathione reductase
multifunctional enzyme involving a fusion of two proteins, glutaredoxin and thioredoxin reductase
glutathione reductase
-
-
-
-
glutathione reductase (NADPH)
-
-
-
-
glutathione S-reductase
-
-
-
-
GOR1
-
-
-
-
GOR2
-
-
-
-
GRase
-
-
-
-
GSH reductase
-
-
-
-
GSSG reductase
-
-
-
-
NADPH-glutathione reductase
-
-
-
-
NADPH-GSSG reductase
-
-
-
-
NADPH:oxidized-glutathione oxidoreductase
-
-
-
-
OBP29
-
-
-
-
reductase, glutathione
-
-
-
-
thioredoxin glutathione reductase
-
chimeric enzyme able to transport reducing equivalents from NADPH to both GSH/GSSG and thioredoxin systems via the fusion of the thioredxin reductase and glutathione reductase domains
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
glutathione:NADP+ oxidoreductase
A dimeric flavoprotein (FAD); activity is dependent on a redox-active disulfide in each of the active centres.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-48-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GSSG + NADPH + H+
GSH + NADP+
show the reaction diagram
-
-
-
?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
show the reaction diagram
-
-
-
-
?
GSSG + NADPH + H+
GSH + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.114 - 0.71
5,5'-dithiobis(2-nitrobenzoic acid)
0.0715 - 0.205
GSSG
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 16
5,5'-dithiobis(2-nitrobenzoic acid)
0.19
GSSG
-
truncated recombinant enzyme (lacking the last two amino acids Sec597-Gly598), in 150 mM potassium phosphate pH 7.0, at 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q962Y6_SCHMA
598
0
64992
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90000
-
SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
x-ray crystallography
dimer
-
2 * 90000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 22% (w/v) PEG 3350, 0.1 M HEPES pH 7.2, 0.2 M NaNO3, 5 mM dithiothreitol, or beta-mercaptoethanol
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Angelucci, F.; Miele, A.E.; Boumis, G.; Dimastrogiovanni, D.; Brunori, M.; Bellelli, A.
Glutathione reductase and thioredoxin reductase at the crossroad: the structure of Schistosoma mansoni thioredoxin glutathione reductase
Proteins
72
936-945
2008
Schistosoma mansoni
Manually annotated by BRENDA team
Sharma, M.; Khanna, S.; Bulusu, G.; Mitra, A.
Comparative modeling of thioredoxin glutathione reductase from Schistosoma mansoni: a multifunctional target for antischistosomal therapy
J. Mol. Graph. Model.
27
665-675
2009
Schistosoma mansoni (Q962Y6), Schistosoma mansoni
Manually annotated by BRENDA team