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Information on EC 1.7.5.2 - nitric oxide reductase (menaquinol)
for references in articles please use BRENDA:EC1.7.5.2
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EC Tree 1 Oxidoreductases
1.7 Acting on other nitrogenous compounds as donors
1.7.5 With a quinone or similar compound as acceptor
1.7.5.2 nitric oxide reductase (menaquinol)
IUBMB Comments
Contains copper.
The expected taxonomic range for this enzyme is: Bacteria, Archaea
- 1.7.5.2
-
15,15'-monooxygenase
- beta-carotene
- lutein
-
carotenoid
- zeaxanthin
-
nitrous
-
inguinal
- azotoformans
- retinal
-
retinoids
-
macula
showSynonyms
menaquinol:no oxidoreductase, menaquinol-dependent nitric oxide reductase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
menaquinol NO reductase
menaquinol:NO oxidoreductase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2 nitric oxide + menaquinol = nitrous oxide + menaquinone + H2O
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
nitrate reduction VII (denitrification)
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
nitric oxide + cytochrome c551
?
-
Substrates: the menaquinol pathway has a 4fold greater maximal activity than the pathway via cytochrome c551
Products: -
Products: -
?
nitric oxide + menaquinol
nitrous oxide + menaquinone + H2O
-
Substrates: -
Products: -
Products: -
?
nitric oxide + menaquinol
nitrous oxide + menaquinone + H2O
-
Substrates: highest activity with menaquinol
Products: -
Products: -
?
nitric oxide + menaquinol
nitrous oxide + menaquinone + H2O
-
Substrates: the menaquinol pathway has a 4fold greater maximal activity than the pathway via cytochrome c551
Products: -
Products: -
?
nitric oxide + menaquinol
nitrous oxide + menaquinone + H2O
Schinkia azotoformans NCCB 100003
-
Substrates: highest activity with menaquinol
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with horse heart ferrocytochrome c
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with lapachol, reduced horse heart cytochrome c, cytochrome c550, and cytochrome c552
Products: -
Products: -
?
additional information
?
-
-
Substrates: the activity using sodium borohydride-reduced menaquinone is about 2times higher than obtained in the presence of ascorbic acid, phenazine methosulfate, and horse heart cytochrome c
Products: -
Products: -
?
additional information
?
-
-
Substrates: the bifunctional qCuANOR can accept electrons from two donors, a specific cytochrome c551 and menaquinol
Products: -
Products: -
?
additional information
?
-
Schinkia azotoformans NCCB 100003
-
Substrates: no activity with horse heart ferrocytochrome c
Products: -
Products: -
?
additional information
?
-
Schinkia azotoformans NCCB 100003
-
Substrates: the activity using sodium borohydride-reduced menaquinone is about 2times higher than obtained in the presence of ascorbic acid, phenazine methosulfate, and horse heart cytochrome c
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
heme
-
the enzyme contains two b-type hemes per enzyme complex. The heme b content of the purified NO reductase is 25.7 nmol/mg. Heme c is absent
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Cu2+
Iron
Cu2+
-
copper A containing enzyme with two copper atoms per enzyme complex, the copper content is 26.7 nmol/mg of protein
Iron
-
the enzyme contains one non-heme iron per enzyme complex. The non-heme iron content is 13.7 nmol/mg
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cytochrome c551
-
noncompetitive inhibitor of nitric oxide reduction when menaquinol is used as an electron donor
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ferricytochrome c
-
NOR activity is stimulated by ferrocytochrome c when phenazine methosulfate and ascorbate are both present
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0045
cytochrome c551
-
pH and temperature not specified in the publication
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
41
nitric oxide
-
using cytochrome c551 as electron donor, pH and temperature not specified in the publication
118
nitric oxide
-
using juglone as electron donor, pH and temperature not specified in the publication
150
nitric oxide
-
using plumbagin as electron donor, pH and temperature not specified in the publication
153
nitric oxide
-
using menaquinol as electron donor, pH and temperature not specified in the publication
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.67
-
crude membrane extract, pH and temperature not specified in the publication
40.7
-
after 60.5fold purification, pH and temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 10
-
approx. 50% of maximal activity at pH 5.0, almost no activity at pH 10.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
-
the ability of qCuANOR to accept electrons from both menaquinol and cytochrome c551 may be related to the regulation of the rate of NO reduction especially as a defense mechanism of B. azotoformans against the toxicity of NO. The menaquinol pathway, which has a 4-fold greater maximal activity than the pathway via cytochrome c551, is used for NO detoxification, whereas electron donation via the endogenous cytochrome c involves the cytochrome b6f complex serving the bioenergetic needs of the organism
physiological function
-
the menaquinol-linked pathway is involved in the detoxification of nitric oxide
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A7ZCA5_CAMC1
Campylobacter concisus (strain 13826)
743
14
83592
TrEMBL
-
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
heterodimer
monomer
monomer
-
1 * 75000-80000, SDS-PAGE, assuming that qNOR binds one dodecyl maltoside micelle of about 40000-50000 Da
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
100
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Q-Sepharose column chromatography, Sephadex G25 gel filtration, and Bio-Scale Ceramic CHT20-I hydroxyapatite column chromatography
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Suharti; Heering, H.A.; de Vries, S.
NO reductase from Bacillus azotoformans is a bifunctional enzyme accepting electrons from menaquinol and a specific endogenous membrane-bound cytochrome c551
Biochemistry
43
13487-13495
2004
Schinkia azotoformans
brenda
de Vries, S.; Strampraad, M.J.; Lu, S.; Moenne-Loccoz, P.; Schroder, I.
Purification and characterization of the MQH2:NO oxidoreductase from the hyperthermophilic archaeon Pyrobaculum aerophilum
J. Biol. Chem.
278
35861-35868
2003
Pyrobaculum aerophilum
brenda
Suharti, S.; de Vries, S.
Membrane-bound denitrification in the Gram-positive bacterium Bacillus azotoformans
Biochem. Soc. Trans.
33
130-133
2005
Schinkia azotoformans
brenda
Suhart, S.; Strampraad, M.; Schrder, I.; De Vries, S.
A novel copper A containing menaquinol NO reductase from Bacillus azotoformans
Biochemistry
40
2632-2639
2001
Schinkia azotoformans, Schinkia azotoformans NCCB 100003
brenda
EXTERNAL LINKS (specific for EC-Number 1.7.5.2)
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Release 2023.1 (January 2023)
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