Information on EC 1.7.5.2 - nitric oxide reductase (menaquinol)

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY hide
1.7.5.2
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RECOMMENDED NAME
GeneOntology No.
nitric oxide reductase (menaquinol)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 nitric oxide + menaquinol = nitrous oxide + menaquinone + H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
nitrate reduction VII (denitrification)
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nitric oxide + ascorbic acid
?
show the reaction diagram
nitric oxide + cytochrome c551
?
show the reaction diagram
nitric oxide + juglone
?
show the reaction diagram
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-
-
-
?
nitric oxide + menaquinol
nitrous oxide + menaquinone + H2O
show the reaction diagram
nitric oxide + plumbagin
?
show the reaction diagram
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-
-
-
?
NO + menaquinol
N2O + H2O + menaquinone
show the reaction diagram
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-
-
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NO + menaquinol
N2O + H2O + menaquinone
show the reaction diagram
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-
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
copper
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the enzyme contains a CuA center in its small subunit
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome c551
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noncompetitive inhibitor of nitric oxide reduction when menaquinol is used as an electron donor
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NO
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substrate inhibition at high concentratins
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ferricytochrome c
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NOR activity is stimulated by ferrocytochrome c when phenazine methosulfate and ascorbate are both present
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0045
cytochrome c551
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pH and temperature not specified in the publication
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0.141
juglone
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pH and temperature not specified in the publication
0.256
Plumbagin
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pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
41 - 153
nitric oxide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.007 - 0.015
NO
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.67
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crude membrane extract, pH and temperature not specified in the publication
3.28
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at 54.5°C
40.7
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after 60.5fold purification, pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
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approx. 50% of maximal activity at pH 5.0, almost no activity at pH 10.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
93
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qNOR in membranes
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 93
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no activity detectable below 25°C
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16000
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1 * 16000 + 1 * 40000, SDS-PAGE
40000
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1 * 16000 + 1 * 40000, SDS-PAGE
78800
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1 * 78800, deduced from nucleotide sequence
112000
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gel filtration
127000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
monomer
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1 * 75000-80000, SDS-PAGE, assuming that qNOR binds one dodecyl maltoside micelle of about 40000-50000 Da; 1 * 78800, deduced from nucleotide sequence
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
no loss of activity after 24 h, qNOR in membrane
56
-
50% loss of activity after 630 min, qNOR in membrane
75
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50% loss of activity after 231 min, qNOR in membrane
100
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50% loss of activity after 4 min, purified qNOR; 50% loss of activity after 86 min, qNOR in membrane
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1% dodecyl maltoside, CM-Sepharose, hydroxyapatite
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Q-Sepharose column chromatography, Sephadex G25 gel filtration, and Bio-Scale Ceramic CHT20-I hydroxyapatite column chromatography
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