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Information on EC 1.7.2.8 - hydrazine dehydrogenase

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EC Tree
IUBMB Comments
The enzyme, which is involved in the pathway of anaerobic ammonium oxidation in anammox bacteria, has been purified from the bacterium Candidatus Kuenenia stuttgartiensis. The electrons derived from hydrazine are eventually transferred to the quinone pool.
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This record set is specific for:
UNIPROT: A0P951
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
hydrazine dehydrogenase, hydrazine oxidoreductase, hydrazine-oxidizing enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrazine-oxidizing enzyme
-
HAO
-
-
-
-
hydrazine oxidoreductase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
hydrazine:ferricytochrome c oxidoreductase
The enzyme, which is involved in the pathway of anaerobic ammonium oxidation in anammox bacteria, has been purified from the bacterium Candidatus Kuenenia stuttgartiensis. The electrons derived from hydrazine are eventually transferred to the quinone pool.
CAS REGISTRY NUMBER
COMMENTARY hide
9075-43-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hydrazine + 2,6-dichloroindophenol
? + reduced 2,6-dichloroindophenol
show the reaction diagram
-
-
-
?
hydrazine + ferricyanide
? + ferrocyanide
show the reaction diagram
-
-
-
?
hydrazine + ferricytochrome c
? + ferrocytochrome c
show the reaction diagram
-
-
-
?
hydrazine + methylthiazolyltetrazolium bromide
? + reduced methylthiazolyltetrazolium bromide
show the reaction diagram
reduction of methylthiazolyltetrazolium bromide via phenazine methosulfate
-
-
?
hydrazine + phenazine methosulfate
? + reduced phenazine methosulfate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
heme
the 62000 Da subunits contains eight heme
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055
hydrazine
pH 8.0, 35°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0024
hydroxylamine
pH 8.0, 35°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
reaction with ferricytochrome c
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0P951_9BACT
568
0
64169
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
gel filtration
62000
2 * 62000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 62000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
35°C, 10 min, stable
695734
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shimamura, M.; Nishiyama, T.; Shigetomo, H.; Toyomoto, T.; Kawahara, Y.; Furukawa, K.; Fujii, T.
Isolation of a multiheme protein with features of a hydrazine-oxidizing enzyme from an anaerobic ammonium-oxidizing enrichment culture
Appl. Environ. Microbiol.
73
1065-1072
2007
Candidatus Jettenia caeni (A0P951), Candidatus Jettenia caeni
Manually annotated by BRENDA team