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Information on EC 1.7.2.4 - nitrous-oxide reductase and Organism(s) Bradyrhizobium diazoefficiens and UniProt Accession Q89XJ6

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IUBMB Comments
The reaction is observed only in the direction of nitrous oxide reduction. Contains the mixed-valent dinuclear CuA species at the electron entry site of the enzyme, and the tetranuclear Cu-Z centre in the active site. In Paracoccus pantotrophus, the electron donor is cytochrome c552.
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Bradyrhizobium diazoefficiens
UNIPROT: Q89XJ6
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The taxonomic range for the selected organisms is: Bradyrhizobium diazoefficiens
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
nitrous oxide reductase, n2o reductase, n(2)or, nitrous-oxide reductase, cnosz, hdn2or, psn2or, z-type n2or, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N(2)OR
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N2O reductase
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nitrous oxide reductase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
nitrogen:cytochrome c oxidoreductase (N2O-forming)
The reaction is observed only in the direction of nitrous oxide reduction. Contains the mixed-valent dinuclear CuA species at the electron entry site of the enzyme, and the tetranuclear Cu-Z centre in the active site. In Paracoccus pantotrophus, the electron donor is cytochrome c552.
CAS REGISTRY NUMBER
COMMENTARY hide
55576-44-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
the level of nosRZD transcripts is severely impaired in the nasT mutant. In the nasT background, deletions of either hairpin leads to increased level of nosRZD transcripts. Under denitrifying conditions, level of nosR, nosZ and nosD transcripts in the nasT mutant severely decrease compared with the nasS mutant and the wild-type, as for nosZ. Phenotypic effects of deletions in the nosR leader region, overview
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene nosZ, expression as nosRZD transcript, quantitative PCR-based expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in Bradyrhizobium diazoefficiens, maximal expression of the nitrous oxide reductase gene nosZ requires oxygen limitation and the presence of a nitrogen oxide. In contrast to nosRZD coding region, nosR-leader transcript level was not affected by nasS or nasT mutations under aerobic or denitrifying conditions respectively. Mapping of two transcription start sites of nosR and identification of two potential hairpins, H1 and H2, within the 5'-leader of nosR transcripts, the two-hairpin RNA structure acts for transcription termination upstream of nosR and the binding of NasT to H1 facilitates read-through transcription to induce nos expression
the putative transcription antiterminator NasT is a positive regulator of nosZ, mechanism overview. But in the absence of nitrate, NasT is counteracted by the nitrate sensor NasS. NasT-mediated mechanism of nosRZDFYLX gene cluster expression, overview. NasT interacts specifically with nosR 5'-leader RNA and that the H1 hairpin contains the binding site critical for such interaction. The terminal loop of the recognition hairpin and the top base pair adjacent to it are important for the interaction with ANTAR proteins
the putative transcription antiterminator NasT is a positive regulator of nosZ; but in the absence of nitrate, NasT is counteracted by the nitrate sensor NasS
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sanchez, C.; Mitsui, H.; Minamisawa, K.
Regulation of nitrous oxide reductase genes by NasT-mediated transcription antitermination in Bradyrhizobium diazoefficiens
Environ. Microbiol. Rep.
9
389-396
2017
Bradyrhizobium diazoefficiens (Q89XJ6), Bradyrhizobium diazoefficiens
Manually annotated by BRENDA team