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Information on EC 1.6.5.4 - monodehydroascorbate reductase (NADH) and Organism(s) Arabidopsis thaliana and UniProt Accession P92947

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Arabidopsis thaliana
UNIPROT: P92947 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
defensin, monodehydroascorbate reductase, mdhar, mdar, afr reductase, mda reductase, ascorbate free radical reductase, trx h2, semidehydroascorbate reductase, monodehydroascorbate radical reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monodehydroascorbate reductase
-
12-oxophytodienoate reductase 3
-
AFR-reductase
-
-
-
-
ascorbate free radical reductase
-
-
-
-
ascorbate free-radical reductase
-
-
-
-
ascorbic free radical reductase
-
-
-
-
MDA reductase
-
-
-
-
MDAsA reductase (NADPH)
-
-
-
-
MDHA
-
-
-
-
monodehydroascorbate reductase
-
monodehydroascorbate reductase 2
-
NADH-semidehydroascorbate oxidoreductase
-
-
-
-
NADH:AFR oxidoreductase
-
-
-
-
NADH:ascorbate radical oxidoreductase
-
-
-
-
NADH:semidehydroascorbic acid oxidoreductase
-
-
-
-
SDA reductase
-
-
-
-
semidehydroascorbate reductase
-
-
-
-
SOR
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
NADH:monodehydroascorbate oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9029-26-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-chloro-2,4-dinitrobenzene + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
?
2,4,6-trinitrotoluene + NADH + H+
? + NAD+
show the reaction diagram
-
-
-
?
monodehydroascorbate + NADH + H+
ascorbate + NAD+
show the reaction diagram
-
-
-
?
2 ferricyanide + NADPH + H+
2 ferrocyanide + NADP+
show the reaction diagram
-
-
-
?
monodehydroascorbate + NADPH + H+
ascorbate + NADP+
show the reaction diagram
-
-
-
?
NADH + monodehydroascorbate
NAD+ + ascorbate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
absorption maxima at 280, 374 and 467 nm
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thioredoxin
the extractable leaf NADPH-dependent MDHAR activity is strongly activated by thioredoxin TRX y2. Activity of recombinant plastid isoform MDHAR6 is more than 2fold increased by reduced TRX y, and not by other plastidial thioredoxins
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.254
1-chloro-2,4-dinitrobenzene
pH not specified in the publication, temperature not specified in the publication
0.522
2,4,6-trinitrotoluene
pH not specified in the publication, temperature not specified in the publication
0.0041
monodehydroascorbate
pH not specified in the publication, temperature not specified in the publication
0.001
NADPH
pH 7.8, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
isoforms with different subcellular localization
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
insertional inactivation of the MDAR2 (At3g09940) gene and the dehydroascorbate reductase 5 (DHAR5, At1g19570) gene shows that they are crucial for maintaining the interaction between Piriformosporaindica and Arabidopsis in a mutualistic state, and under drought stress in particular
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MDAR_ARATH
493
0
53302
Swiss-Prot
Chloroplast (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
-
MDAR1, SDS-PAGE
54000
-
MDAR4, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 42000, SDS-PAGE, x * 42690, calculated from sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA agarose chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression of mitochondrial and chloroplastic isozymes as GFP-fusion proteins in transgenic Arabidopsis plants, targeting of recombinant fusion proteins to the organelles in vivo in transgenic plants, dual targeting is achieved by multiple transcription initiation sites, 2 transcripts, a small and a large one, both have an N-terminal extension
expressed in Nicotiana tabacum
overexpression in Nicotiana tabacum. The transgenic plants exhibit up to 2.1fold higher monodehydroascorbate reductase activity and 2.2fold higher level of reduced ascorbate compared to non-transformed control plants. The transgenic plants show enhanced stress tolerance in term of significantly higher net photosynthesis rates under ozone, salt and polyethylene glycol stresses and greater PSII effective quantum yield under ozone and salt stresses
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ascorbate, the MDAR2 (At3g09940) and the dehydroascorbate reductase 5 (DHAR5, At1g19570) mRNA levels are upregulated in Arabidopsis roots colonized by the beneficial endophytic fungus Piriformosporaindica
exposure to 0.4 mM aluminium decreases the activity of MDAR
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Obara, K.; Sumi, K.; Fukuda, H.
The use of multiple transcription starts causes the dual targeting of Arabidopsis putative monodehydroascorbate reductase to both mitochondria and chloroplasts
Plant Cell Physiol.
43
697-705
2002
Arabidopsis thaliana (P92947)
Manually annotated by BRENDA team
Lisenbee, C.S.; Lingard, M.J.; Trelease, R.N.
Arabidopsis peroxisomes possess functionally redundant membrane and matrix isoforms of monodehydroascorbate reductase
Plant J.
43
900-914
2005
Arabidopsis thaliana
Manually annotated by BRENDA team
Eltayeb, A.E.; Kawano, N.; Badawi, G.H.; Kaminaka, H.; Sanekata, T.; Shibahara, T.; Inanaga, S.; Tanaka, K.
Overexpression of monodehydroascorbate reductase in transgenic tobacco confers enhanced tolerance to ozone, salt and polyethylene glycol stresses
Planta
225
1255-1264
2007
Arabidopsis thaliana, Arabidopsis thaliana (Q9SR59)
Manually annotated by BRENDA team
Vadassery, J.; Tripathi, S.; Prasad, R.; Varma, A.; Oelmueller, R.
Monodehydroascorbate reductase 2 and dehydroascorbate reductase 5 are crucial for a mutualistic interaction between Piriformospora indica and Arabidopsis
J. Plant Physiol.
166
1263-1274
2009
Arabidopsis thaliana (Q9LK94)
Manually annotated by BRENDA team
Yin, L.; Wang, S.; Eltayeb, A.E.; Uddin, M.I.; Yamamoto, Y.; Tsuji, W.; Takeuchi, Y.; Tanaka, K.
Overexpression of dehydroascorbate reductase, but not monodehydroascorbate reductase, confers tolerance to aluminum stress in transgenic tobacco
Planta
231
609-621
2010
Arabidopsis thaliana
Manually annotated by BRENDA team
Johnston, E.; Rylott, E.; Beynon, E.; Lorenz, A.; Chechik, V.; Bruce, N.
Monodehydroascorbate reductase mediates TNT toxicity in plants
Science
349
1072-1075
2015
Arabidopsis thaliana (P92947)
-
Manually annotated by BRENDA team
Vanacker, H.; Guichard, M.; Bohrer, A.S.; Issakidis-Bourguet, E.
Redox regulation of monodehydroascorbate reductase by thioredoxin y in plastids revealed in the context of water stress
Antioxidants (Basel)
7
183
2018
Arabidopsis thaliana (P92947)
Manually annotated by BRENDA team
Maynard, D.; Kumar, V.; Sproi, J.; Dietz, K.J.
12-Oxophytodienoic acid reductase 3 (OPR3) functions as NADPH-dependent alpha,beta-ketoalkene reductase in detoxification and monodehydroascorbate reductase in redox homeostasis
Plant Cell Physiol.
61
584-595
2020
Arabidopsis thaliana (Q9FUP0)
Manually annotated by BRENDA team