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Information on EC 1.6.2.2 - cytochrome-b5 reductase and Organism(s) Mus musculus and UniProt Accession Q3TDX8

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EC Tree
     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.2 cytochrome-b5 reductase
IUBMB Comments
A flavoprotein (FAD).
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This record set is specific for:
Mus musculus
UNIPROT: Q3TDX8
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
nadh-cytochrome b5 reductase, cytochrome b5 reductase, methemoglobin reductase, cyb5r3, ncb5or, nadh cytochrome b5 reductase, nadh:cytochrome b5 reductase, cyb5r2, cytochrome-b5 reductase, cyb5r, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cytochrome b5 reductase
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-
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dihydronicotinamide adenine dinucleotide-cytochrome b5 reductase
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-
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NADH-cytochrome b 5 reductase
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-
NADH-cytochrome b5 reductase
NADH-cytochrome-b5 reductase
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-
-
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NADH-ferricytochrome b5 oxidoreductase
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-
-
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P34/P32
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-
-
-
P35
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-
-
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reduced nicotinamide adeninedinucleotide-cytochrome b5 reductase
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-
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reductase, cytochrome b5
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
NADH:ferricytochrome-b5 oxidoreductase
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
9032-25-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome b5 + NADH
2 ferrocytochrome b5 + NAD+ + H+
show the reaction diagram
1,2-dihydro-8-(4-methylpiperazin-1-yl)-4-phenylimidazol[3,2-e]pyrazine 5-oxide + NADH
1,2-dihydro-8-(4-methylpiperazin-1-yl)-4-phenylimidazol[3,2-e]pyrazine + NAD+
show the reaction diagram
-
potential bioreductive drug, trivial name RB90740
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?
NADH + ferricytochrome b5
NAD+ + H+ + ferrocytochrome b5
show the reaction diagram
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-
-
-
?
NADPH + ferricytochrome b5
NADP+ + H+ + ferrocytochrome b5
show the reaction diagram
-
low affinity for NADPH
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome b5 + NADH
2 ferrocytochrome b5 + NAD+ + H+
show the reaction diagram
involved in the synthesis of fatty acids and cholesterol, and in the oxidation of xenobiotics, removal of reactive oxygen species
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-
?
NADH + ferricytochrome b5
NAD+ + H+ + ferrocytochrome b5
show the reaction diagram
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-
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activates
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-propyl-2-thiouracil
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25 mM, almost complete inhibition
p-hydroxymercuribenzoate
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0.1 mM, almost complete inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00072
-
liver microsomes, reduction of 1,2-dihydro-8-(4-methylpiperazin-1-yl)-4-phenylimidazol[3,2-e]pyrazine 5-oxide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cytochrome b5/cytochrome b5 reductase fusion protein, exists also as a variant where the whole exon 12 is deleted
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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cytochrome b5/cytochrome b5 reductase can act as a sole electron donor to the P450 system in vitro and in vivo
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NB5R4_MOUSE
528
0
59716
Swiss-Prot
Mitochondrion (Reliability: 5)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression and protein abundance is clearly decreased during fasting
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Barham, H.M.; Stratford, I.J.
Enzymology of the reduction of the novel fused pyrazine mono-N-oxide bioreductive drug, RB90740 roles for P450 reductase and cytochrome b5 reductase
Biochem. Pharmacol.
51
829-837
1996
Mus musculus
Manually annotated by BRENDA team
Curry, B.J.; Roman, S.D.; Wallace, C.A.; Scott, R.; Miriami, E.; Aitken, R.J.
Identification and characterization of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase
Genomics
83
425-438
2004
Mus musculus (Q3TDX8), Mus musculus, Rattus norvegicus (Q68EJ0)
Manually annotated by BRENDA team
Oikawa, K.; Odero, G.L.; Nafez, S.; Ge, N.; Zhang, D.; Kobayashi, H.; Sate, K.; Kimura, S.; Tateno, M.; Albensi, B.C.
Visinin-like protein-3 modulates the interaction between cytochrome b 5 and NADH-cytochrome b 5 reductase in a Ca(2+)-dependent manner
Cell Biochem. Biophys.
74
449-457
2016
Mus musculus
Manually annotated by BRENDA team
Henderson, C.J.; McLaughlin, L.A.; Wolf, C.R.
Evidence that cytochrome b5 and cytochrome b5 reductase can act as sole electron donors to the hepatic cytochrome P450 system
Mol. Pharmacol.
83
1209-1217
2013
Mus musculus
Manually annotated by BRENDA team
Jakobs, H.H.; Mikula, M.; Havemeyer, A.; Strzalkowska, A.; Borowa-Chmielak, M.; Dzwonek, A.; Gajewska, M.; Hennig, E.E.; Ostrowski, J.; Clement, B.
The N-reductive system composed of mitochondrial amidoxime reducing component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase (CYB5R) is regulated by fasting and high fat diet in mice
PLoS ONE
9
e105371
2014
Mus musculus
Manually annotated by BRENDA team