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Information on EC 1.5.99.6 - spermidine dehydrogenase
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1.5.99.6 spermidine dehydrogenaseIUBMB Comments
A flavohemoprotein (FAD). Ferricyanide, 2,6-dichloroindophenol and cytochrome c can act as acceptor. 4-Aminobutanal condenses non-enzymically to 1-pyrroline.
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Word Map
- 1.5.99.6
- marcescens
-
serratia
- 1,3-diaminopropane
- amines
-
methylene
- polyamines
- triamine
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
spermidine dehydrogenase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
spermidine + acceptor + H2O = propane-1,3-diamine + 4-aminobutanal + reduced acceptor
A flavohemoprotein (FAD). Ferricyanide, 2,6-dichloroindophenol and cytochrome c can act as acceptor. 4-aminobutanal condenses non-enzymically to 1-pyrroline
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
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redox reaction
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-
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reduction
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-
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-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
spermidine:acceptor oxidoreductase
A flavohemoprotein (FAD). Ferricyanide, 2,6-dichloroindophenol and cytochrome c can act as acceptor. 4-Aminobutanal condenses non-enzymically to 1-pyrroline.
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CAS REGISTRY NUMBER
COMMENTARY
9076-64-6
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3,3'-diaminodipropylamine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
monoacetylspermidine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
N,N'-bis(3-aminopropyl)-1,3-propanediamine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
N,N'-di(aminopropyl)-propanediamine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
N-(3-aminopropyl)-1,3-diaminopropane + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
N-(3-aminopropyl)-1,3-propanediamine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
N-(3-hydroxypropyl)-1,4-diaminobutane + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
N-alkylputrescine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
N8-acetylspermidine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
spermidine + dichlorophenolindophenol
1,3-diaminopropane + 4-aminobutyraldehyde + reduced dichlorophenolindophenol
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dichlorophenolindophenol results in 10% of the efficiency of ferricyanide
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-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
spermidine + ferricyanide
1,3-diaminopropane + 4-aminobutyraldehyde + ferrocyanide
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-
-
-
?
spermidine + phenazine methosulfate
1,3-diaminopropane + 4-aminobutyraldehyde + reduced phenazine methosulfate
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phenazine methosulfate results in 10% of the efficiency of ferricyanide
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-
?
spermidine + phenazine methosulfate
1,3-diaminopropane + DELTA1-pyrroline + reduced phenazine methosulfate
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-
-
-
?
spermine + dichlorophenolindophenol
spermidine + 3-aminopropionaldehyde + reduced dichlorophenolindophenol
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dichlorophenolindophenol results in 10% of the efficiency of ferricyanide
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-
?
spermine + ferricyanide
spermidine + 3-aminopropionaldehyde + ferrocyanide
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-
-
-
?
spermine + phenazine methosulfate
spermidine + 3-aminopropionaldehyde + reduced phenazine methosulfate
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phenazine methosulfate results in 10% of the efficiency of ferricyanide
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-
?
additional information
?
-
-
Pseudomonas aeruginosa does not produce sufficient amounts of the enzyme to perform a catabolic role in vivo
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-
?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
spermidine + 2,6-dichlorophenolindophenol
1,3-diaminopropane + DELTA1-pyrroline + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
spermidine + cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
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-
-
-
?
spermidine + cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
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natural electron acceptor horse heart cytochrome c
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-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
Flavobacterium dormitator
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-
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?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
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-
-
-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
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-
-
-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
-
?
spermidine + electron acceptor
1,3-diaminopropane + DELTA1-pyrroline + reduced electron acceptor
-
-
-
-
?
spermidine + K3Fe(CN)6
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
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-
-
-
?
spermidine + K3Fe(CN)6
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
-
?
spermidine + K3Fe(CN)6
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
-
?
spermidine + K3Fe(CN)6
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
-
-
-
-
?
spermidine + K3Fe(CN)6
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
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-
-
-
?
spermidine + K3Fe(CN)6
1,3-diaminopropane + DELTA1-pyrroline + ferrocyanide
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-
-
-
?
spermine + electron acceptor
? + reduced electron acceptor
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-
-
-
?
spermine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
spermine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
spermine + electron acceptor
? + reduced electron acceptor
-
oxidized slowly
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?
spermine + electron acceptor
? + reduced electron acceptor
-
-
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Pseudomonas aeruginosa does not produce sufficient amounts of the enzyme to perform a catabolic role in vivo
-
-
?
spermidine + cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
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-
-
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?
spermidine + cytochrome c
1,3-diaminopropane + gamma-aminobutylaldehyde
-
natural electron acceptor horse heart cytochrome c
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
at optimal pH Km for spermidine less than 0.0001 mM
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.8
1,4-diaminooctane
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does not inhibit the enzyme when spermidine is used as substrate
0.34
1,6-diaminooctane
-
does not inhibit the enzyme when spermidine is used as substrate
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.2
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
membrane-bound, outer surface of the cytoplasmic membranes or periplasmic space of the organism
brenda
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membrane-bound, outer surface of the cytoplasmic membranes or periplasmic space of the organism
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brenda
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A840NZ03_9ACTN
625
0
67595
TrEMBL
-
Q9HXS8_PSEAE
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
620
0
68862
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
43000
63000
65000
76000
76000
-
sedimentation equilibrium centrifugation, centrifugation in a sucrose density gradient
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, can be stored in 0.1 M potassium phosphate buffer, pH 7.2 for over 6 months with less than 10% loss of activity
-
-20°C, can be stored in supernatant solution for several weeks without loss of activity
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE