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Information on EC 1.5.99.5 - methylglutamate dehydrogenase
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1.5.99.5 methylglutamate dehydrogenaseIUBMB Comments
A number of N-methyl-substituted amino acids can act as donor; 2,6-dichloroindophenol is the best acceptor.
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Word Map
- 1.5.99.5
- methylamine
- aminovorans
-
gamma-glutamylmethylamide
- monomethylamine
-
facultative
-
methylobacterium
- triton
-
extorquens
- sarcosine
- formaldehyde
The enzyme appears in viruses and cellular organisms
Synonyms
n-methylglutamate dehydrogenase, nmg dehydrogenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
N-methyl-L-glutamate:oxidoreductase
N-methylglutamate dehydrogenase
NMG dehydrogenase
NMGDH
N-methylglutamate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
N-methyl-L-glutamate + acceptor + H2O = L-glutamate + formaldehyde + reduced acceptor
a number od N-methyl-substituted amino acids can act as donor, 2,6-dichloroindophenol is the best acceptor
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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oxidative deamination
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redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
N-methyl-L-glutamate:acceptor oxidoreductase (demethylating)
A number of N-methyl-substituted amino acids can act as donor; 2,6-dichloroindophenol is the best acceptor.
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CAS REGISTRY NUMBER
COMMENTARY
37217-26-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-methyl D-alanine + 2,6-dichlorophenolindophenol + H2O
D-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
N-methyl DL-valine + 2,6-dichlorophenolindophenol + H2O
DL-valine + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
N-methyl L-isoleucine + 2,6-dichlorophenolindophenol + H2O
L-isoleucine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
N-methyl L-phenylalanine + 2,6-dichlorophenolindophenol + H2O
L-phenylalanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
N-methyl L-serine + 2,6-dichlorophenolindophenol + H2O
L-serine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
N-methyl-DL-glutamate + 2,6-dichlorophenolindophenol + H2O
DL-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
bacterium AT2
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-
-
?
N-methyl-glycine + 2,6-dichlorophenolindophenol + H2O
glycine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
N-methylglutamate + horse heart cytochrome c + H2O
L-glutamate + formaldehyde + reduced horse heart cytochrome c
N-methylglutamate + N,N,N,N-tetramethyl-o-phenylene diamine + H2O
L-glutamate + formaldehyde + reduced N,N,N,N-tetramethyl-o-phenylene diamine
bacterium AT2
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-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
N-methylglutamate + potassium ferricyanide + H2O
L-glutamate + formaldehyde + reduced potassium ferricyanide
N-methylglutamate + radical cation of 2,2'-azinodi-[3-ethylbenzthiazoline 6-sulfonate] + H2O
L-glutamate + formaldehyde + reduced acceptor
bacterium AT2
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-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
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?
N-methyl DL-alanine + 2,6-dichlorophenolindophenol + H2O
DL-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
bacterium AT2
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-
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?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
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?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
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?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
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?
N-methyl DL-aspartate + 2,6-dichlorophenolindophenol + H2O
DL-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
bacterium AT2
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-
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?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
bacterium AT2
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?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
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?
N-methyl L-alanine + 2,6-dichlorophenolindophenol + H2O
L-alanine + formaldehyde + reduced 2,6-dichlorophenolindophenol
Pseudomonas sp. MA / M.A. / ATCC 23819
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?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
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?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
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?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
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?
N-methyl L-aspartate + 2,6-dichlorophenolindophenol + H2O
L-aspartate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
bacterium AT2
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
Pseudomonas methylica
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
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?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
Pseudomonas sp. MA / M.A. / ATCC 23819
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?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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the enzyme shows monomethylamine dehydrogenase activity with methylamine hydrochloride as substrate, assay using 2,6-dichlorophenolindophenol (DCPIP) as an artificial electron acceptor with or without KCN
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?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
the enzyme shows monomethylamine dehydrogenase activity with methylamine hydrochloride as substrate, assay using 2,6-dichlorophenolindophenol (DCPIP) as an artificial electron acceptor with or without KCN
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?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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?
N-methyl-L-glutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
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?
N-methylglutamate + cytochrome c + H2O
L-glutamate + formaldehyde + reduced cytochrome c
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-
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?
N-methylglutamate + cytochrome c + H2O
L-glutamate + formaldehyde + reduced cytochrome c
Pseudomonas sp. MA / M.A. / ATCC 23819
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?
N-methylglutamate + horse heart cytochrome c + H2O
L-glutamate + formaldehyde + reduced horse heart cytochrome c
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ir
N-methylglutamate + horse heart cytochrome c + H2O
L-glutamate + formaldehyde + reduced horse heart cytochrome c
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ir
N-methylglutamate + O2
L-glutamate + formaldehyde + H2O
Pseudomonas sp. MA / M.A. / ATCC 23819
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ir
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
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?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
bacterium AT2
-
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
N-methylglutamate + oxidized 2,6-dichlorophenolindophenol + H2O
L-glutamate + formaldehyde + reduced 2,6-dichlorophenolindophenol
Pseudomonas sp. MA / M.A. / ATCC 23819
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-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
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-
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?
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
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?
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
bacterium AT2
-
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
-
-
-
-
?
N-methylglutamate + phenazine methosulfate + H2O
L-glutamate + formaldehyde + reduced phenazine methosulfate
Pseudomonas sp. MA / M.A. / ATCC 23819
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-
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?
N-methylglutamate + potassium ferricyanide + H2O
L-glutamate + formaldehyde + reduced potassium ferricyanide
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-
-
?
N-methylglutamate + potassium ferricyanide + H2O
L-glutamate + formaldehyde + reduced potassium ferricyanide
Pseudomonas sp. MA / M.A. / ATCC 23819
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-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
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-
-
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?
sarcosine + 2,6-dichlorophenolindophenol + H2O
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
bacterium AT2
-
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
sarcosine + 2,6-dichlorophenolindophenol + H2O
aminoacetate + formaldehyde + reduced 2,6-dichlorophenolindophenol
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-
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?
additional information
?
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L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+
-
-
?
additional information
?
-
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L-glutamate is no substrate, mono-, di- or tri-methylamine, trimethylamine N-oxide or tetramethylammonium chloride are not oxidized, no N-methylglutamate-dependent reduction of ferricyanide, NAD+ or NADP+
-
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?
additional information
?
-
bacterium AT2
-
not active with NAD+, ferricyanide cytochrome c or horse heart cytochrome c, no activity detected with N-methyl-D-alanine
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?
additional information
?
-
-
L-glutamate, methylamine, formaldehyde and gamma-glutaryl-N-methylamide are no substrates
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?
additional information
?
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
L-glutamate, methylamine, formaldehyde and gamma-glutaryl-N-methylamide are no substrates
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?
additional information
?
-
Pseudomonas sp. MA / M.A. / ATCC 23819
-
N,N-dimethylglutamate is no substrate
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
bacterium AT2
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
Pseudomonas methylica
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
-
-
-
-
?
N-methyl-L-glutamate + acceptor + H2O
L-glutamate + formaldehyde + reduced acceptor
Pseudomonas sp. MA / M.A. / ATCC 23819
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
-
-
brenda
genes mgdA, mgdB, and mgdC, encoding subunits A, B, and large subunit c
-
-
brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
additional information
metabolism
-
SoxBDAG, encoding sarcosine dehydrogenase,may be involved in oxidation of N-methyl-L-glutamate, overview
metabolism
-
the bacterium utilizes a methylated amino acid pathway gamma-glutamylmethylamide and N-methylglutamate for monomethylamine metabolism. The two key enzymes in this pathway are gamma-glutamylmethylamide synthetase and N-methylglutamate dehydrogenase. The gamma-glutamylmethylamide synthetase is essential for the pathway
metabolism
-
SoxBDAG, encoding sarcosine dehydrogenase,may be involved in oxidation of N-methyl-L-glutamate, overview
-
metabolism
-
the bacterium utilizes a methylated amino acid pathway gamma-glutamylmethylamide and N-methylglutamate for monomethylamine metabolism. The two key enzymes in this pathway are gamma-glutamylmethylamide synthetase and N-methylglutamate dehydrogenase. The gamma-glutamylmethylamide synthetase is essential for the pathway
-
additional information
-
the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway in the methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5. The enzyme contains an aminomethyltransferase folate-binding domain near the C-terminus of its large subunit
additional information
-
the enzyme is encoded in a gene cluster essential for operation of the N-methylglutamate pathway in the methylotrophic beta-proteobacterium Methyloversatilis universalis FAM5. The enzyme contains an aminomethyltransferase folate-binding domain near the C-terminus of its large subunit
-
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UNIPROT
ENTRY NAME
ORGANISM