Information on EC 1.5.98.3 - coenzyme F420:methanophenazine dehydrogenase

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The expected taxonomic range for this enzyme is: Methanosarcinales

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EC NUMBER
COMMENTARY hide
1.5.98.3
-
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RECOMMENDED NAME
GeneOntology No.
coenzyme F420:methanophenazine dehydrogenase
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
reduced coenzyme F420 + methanophenazine = oxidized coenzyme F420 + dihydromethanophenazine
show the reaction diagram
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme B/coenzyme M regeneration I (methanophenazine-dependent)
-
-
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SYSTEMATIC NAME
IUBMB Comments
reduced coenzyme F420:methanophenazine oxidoreductase
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oxidized coenzyme F420 + reduced 2,3-dimethyl-1,4-naphthoquinone
reduced coenzyme F420 + oxidized 2,3-dimethyl-1,4-naphthoquinone
show the reaction diagram
oxidized coenzyme F420 + reduced 2-bromophenazine
reduced coenzyme F420 + oxidized 2-bromophenazine
show the reaction diagram
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
reduced coenzyme F420 + oxidized 2-hydroxyphenazine
show the reaction diagram
oxidized coenzyme F420 + reduced ferredoxin
reduced coenzyme F420 + oxidized ferredoxin
show the reaction diagram
oxidized coenzyme F420 + reduced methyl viologen
reduced coenzyme F420 + oxidized methyl viologen
show the reaction diagram
oxidized coenzyme F420 + reduced phenazine
reduced coenzyme F420 + oxidized phenazine
show the reaction diagram
-
-
-
-
?
oxidized coenzyme F420 + reduced tetramethyl-4-benzoquinone
reduced coenzyme F420 + oxidized tetramethyl-4-benzoquinone
show the reaction diagram
-
-
-
-
?
reduced coenzyme F420 + 2-bromophenazine
?
show the reaction diagram
-
-
-
-
?
reduced coenzyme F420 + 2-hydroxyphenazine
?
show the reaction diagram
-
-
-
-
?
reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
show the reaction diagram
reduced coenzyme F420 + methyl viologen
?
show the reaction diagram
-
-
-
-
?
reduced coenzyme F420 + oxidized 2-hydroxyphenazine
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
show the reaction diagram
reduced coenzyme F420 + oxidized metronidazole
oxidized coenzyme F420 + reduced metronidazole
show the reaction diagram
reduced coenzyme F420 + phenazine
?
show the reaction diagram
-
-
-
-
?
reduced coenzyme F420 + phenazine-1-carboxylic acid
?
show the reaction diagram
-
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iron-sulfur center
-
the enzyme contains iron-sulfur clusters
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
16 mol non-heme iron per mol enzyme
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.035
2-hydroxyphenazine
-
at pH 7.0 and 25°C
0.25
phenazine
-
at pH 7.0 and 25°C
0.0054
reduced coenzyme F420
-
at pH 6.8 and 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
25.5
reduced coenzyme F420
-
at pH 6.8 and 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.6
-
with reduced tetramethyl-4-benzoquinone as cosubstrate, at pH 7.0 and 37°C
1.1
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with reduced 2,3-dimethyl-1,4-naphthoquinone as cosubstrate, at pH 7.0 and 37°C; with reduced methyl viologen as cosubstrate, at pH 7.0 and 37°C; with reduced phenazine as cosubstrate, at pH 7.0 and 37°C
1.7
-
with reduced 2-bromophenazine as cosubstrate, at pH 7.0 and 37°C
1.8
-
with phenazine as cosubstrate, at pH 7.0 and 25°C
4.6
-
with phenazine-1-carboxylic acid as cosubstrate, at pH 7.0 and 25°C
8.4
-
with 2-bromophenazine as cosubstrate, at pH 7.0 and 25°C
8.8
-
with 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 25°C
10
-
with methyl viologen as cosubstrate, at pH 7.0 and 25°C
10.4
-
with reduced 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
isoelectric focusing
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
-
gel filtration
120000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
the purified enzyme becomes rapidly inactivated by repeated freezing and thawing
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sephacel column chromatography and EAH-Sepharose column chromatography
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LINKS TO OTHER DATABASES (specific for EC-Number 1.5.98.3)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)