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Information on EC 1.5.98.3 - coenzyme F420:methanophenazine dehydrogenase for references in articles please use BRENDA:EC1.5.98.3
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EC Tree
The expected taxonomic range for this enzyme is: Methanosarcinales
Synonyms
f420h2-dehydrogenase,
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F420-phenazine oxidoreductase
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F420H2 dehydrogenase
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F420H2 dehydrogenase
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F420H2 dehydrogenase
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F420H2-dehydrogenase
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F420H2-dehydrogenase
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ffd
gene name
Fpo
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FpoAHJKLMN
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gene name
fpoBCDIF
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reduced coenzyme F420 + methanophenazine = oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420:methanophenazine oxidoreductase
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oxidized coenzyme F420 + reduced 2,3-dimethyl-1,4-naphthoquinone
reduced coenzyme F420 + oxidized 2,3-dimethyl-1,4-naphthoquinone
oxidized coenzyme F420 + reduced 2-bromophenazine
reduced coenzyme F420 + oxidized 2-bromophenazine
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
reduced coenzyme F420 + oxidized 2-hydroxyphenazine
oxidized coenzyme F420 + reduced ferredoxin
reduced coenzyme F420 + oxidized ferredoxin
oxidized coenzyme F420 + reduced methyl viologen
reduced coenzyme F420 + oxidized methyl viologen
oxidized coenzyme F420 + reduced phenazine
reduced coenzyme F420 + oxidized phenazine
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oxidized coenzyme F420 + reduced tetramethyl-4-benzoquinone
reduced coenzyme F420 + oxidized tetramethyl-4-benzoquinone
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reduced coenzyme F420 + 2-bromophenazine
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reduced coenzyme F420 + 2-hydroxyphenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
reduced coenzyme F420 + methyl viologen
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reduced coenzyme F420 + oxidized 2-hydroxyphenazine
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
reduced coenzyme F420 + oxidized metronidazole
oxidized coenzyme F420 + reduced metronidazole
reduced coenzyme F420 + phenazine
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reduced coenzyme F420 + phenazine-1-carboxylic acid
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oxidized coenzyme F420 + reduced 2,3-dimethyl-1,4-naphthoquinone
reduced coenzyme F420 + oxidized 2,3-dimethyl-1,4-naphthoquinone
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oxidized coenzyme F420 + reduced 2,3-dimethyl-1,4-naphthoquinone
reduced coenzyme F420 + oxidized 2,3-dimethyl-1,4-naphthoquinone
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oxidized coenzyme F420 + reduced 2-bromophenazine
reduced coenzyme F420 + oxidized 2-bromophenazine
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oxidized coenzyme F420 + reduced 2-bromophenazine
reduced coenzyme F420 + oxidized 2-bromophenazine
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oxidized coenzyme F420 + reduced 2-hydroxyphenazine
reduced coenzyme F420 + oxidized 2-hydroxyphenazine
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oxidized coenzyme F420 + reduced 2-hydroxyphenazine
reduced coenzyme F420 + oxidized 2-hydroxyphenazine
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oxidized coenzyme F420 + reduced ferredoxin
reduced coenzyme F420 + oxidized ferredoxin
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oxidized coenzyme F420 + reduced ferredoxin
reduced coenzyme F420 + oxidized ferredoxin
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oxidized coenzyme F420 + reduced methyl viologen
reduced coenzyme F420 + oxidized methyl viologen
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oxidized coenzyme F420 + reduced methyl viologen
reduced coenzyme F420 + oxidized methyl viologen
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + oxidized 2-hydroxyphenazine
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
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reduced coenzyme F420 + oxidized 2-hydroxyphenazine
oxidized coenzyme F420 + reduced 2-hydroxyphenazine
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reduced coenzyme F420 + oxidized metronidazole
oxidized coenzyme F420 + reduced metronidazole
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reduced coenzyme F420 + oxidized metronidazole
oxidized coenzyme F420 + reduced metronidazole
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
Q9UXN9
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
Q9UXN9
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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reduced coenzyme F420 + methanophenazine
oxidized coenzyme F420 + dihydromethanophenazine
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iron-sulfur center
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the enzyme contains iron-sulfur clusters
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Iron
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16 mol non-heme iron per mol enzyme
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0.035
2-hydroxyphenazine
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at pH 7.0 and 25°C
0.25
phenazine
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at pH 7.0 and 25°C
0.0054
reduced coenzyme F420
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at pH 6.8 and 37°C
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25.5
reduced coenzyme F420
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at pH 6.8 and 37°C
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0.6
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with reduced tetramethyl-4-benzoquinone as cosubstrate, at pH 7.0 and 37°C
1.1
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with reduced 2,3-dimethyl-1,4-naphthoquinone as cosubstrate, at pH 7.0 and 37°C; with reduced methyl viologen as cosubstrate, at pH 7.0 and 37°C; with reduced phenazine as cosubstrate, at pH 7.0 and 37°C
1.7
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with reduced 2-bromophenazine as cosubstrate, at pH 7.0 and 37°C
1.8
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with phenazine as cosubstrate, at pH 7.0 and 25°C
4.6
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with phenazine-1-carboxylic acid as cosubstrate, at pH 7.0 and 25°C
8.4
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with 2-bromophenazine as cosubstrate, at pH 7.0 and 25°C
8.8
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with 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 25°C
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with methyl viologen as cosubstrate, at pH 7.0 and 25°C
10.4
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with reduced 2-hydroxyphenazine as cosubstrate, at pH 7.0 and 37°C
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brenda
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brenda
40 kDa subunit
UniProt
brenda
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brenda
40 kDa subunit
UniProt
brenda
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brenda
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malfunction
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knockout mutants of the membrane-bound F420 dehydrogenase exhibit severe growth deficiencies with trimethylamine, but not with acetate, as substrates
malfunction
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knockout mutants of the membrane-bound F420 dehydrogenase exhibit severe growth deficiencies with trimethylamine, but not with acetate, as substrates
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metabolism
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the enzyme plays a major role in the oxidative part of methylotrophic methanogenesis
metabolism
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the enzyme plays a major role in the oxidative part of methylotrophic methanogenesis
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Q9UXN9_METTI
342
0
37641
TrEMBL
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x * 45000 + x * 40000 + x * 22000 + x * 18000 + x * 17000, SDS-PAGE
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x * 40000, SDS-PAGE; x * 45000 + x * 40000 + x * 22000 + x * 18000 + x * 17000, SDS-PAGE
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the purified enzyme becomes rapidly inactivated by repeated freezing and thawing
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DEAE-Sephacel column chromatography and EAH-Sepharose column chromatography
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Haase, P.; Deppenmeier, U.; Blaut, M.; Gottschalk, G.
Purification and characterization of F420H2-dehydrogenase from Methanolobus tindarius
Eur. J. Biochem.
203
527-531
1992
Methanolobus tindarius, Methanolobus tindarius DSM 2278
brenda
Welte, C.; Deppenmeier, U.
Re-evaluation of the function of the F420 dehydrogenase in electron transport of Methanosarcina mazei
FEBS J.
278
1277-1287
2011
Methanosarcina mazei, Methanosarcina mazei DSM 7222
brenda
Baeumer, S.; Murakami, E.; Brodersen, J.; Gottschalk, G.; Ragsdale, S.; Deppenmeier, U.
The F420H2heterodisulfide oxidoreductase system from Methanosarcina species. 2-Hydroxyphenazine mediates electron transfer from F420H2 dehydrogenase to heterodisulfide reductase
FEBS Lett.
428
295-298
1998
Methanosarcina mazei, Methanosarcina mazei Goe1
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brenda
Westenberg, D.; Braune, A.; Ruppert, C.; Müller, V.; Herzberg, C.; Gottschalk, G.; Blaut, M.
The F420H2-dehydrogenase from Methanolobus tindarius cloning of the ffd operon and expression of the genes in Escherichia coli
FEMS Microbiol. Lett.
170
389-398
1999
Methanolobus tindarius, Methanolobus tindarius (Q9UXN9), Methanolobus tindarius DSM 2278 (Q9UXN9)
brenda
Wang, F.P.; Zhang, Y.; Chen, Y.; He, Y.; Qi, J.; Hinrichs, K.U.; Zhang, X.X.; Xiao, X.; Boon, N.
Methanotrophic archaea possessing diverging methane-oxidizing and electron-transporting pathways
ISME J.
8
1069-1078
2014
Methanosarcina sp.
brenda
Abken, H.; Tietze, M.; Brodersen, J.; Baumer, S.; Beifuss, U.; Deppenmeier, U.
Isolation and characterization of methanophenazine and function of phenazines in membrane-bound electron transport of Methanosarcina mazei Go1
J. Bacteriol.
180
2027-2032
1998
Methanosarcina mazei
brenda
Welte, C.; Deppenmeier, U.
Membrane-bound electron transport in Methanosaeta thermophile
J. Bacteriol.
192
2868-2870
2011
Methanothrix thermoacetophila
brenda
Baumer, S.; Ide, T.; Jacobi, C.; Johann, A.; Gottschalk, G.; Deppenmeier, U.
The F420H2 dehydrogenase from Methanosarcina mazei is a redox-driven proton pump closely related to NADH dehydrogenases
J. Biol. Chem.
275
17968-17973
2000
Methanosarcina mazei, Methanosarcina mazei Goe1
brenda
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1.5.98.3 coenzyme F420:methanophenazine dehydrogenase
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