This bacterial enzyme is involved in degradation of glycine betaine. The enzyme contains non-covalently bound FAD and NAD(P) cofactors, and catalyses the demethylation of both N,N-dimethylglycine and sarcosine, releasing formaldehyde and forming glycine as the final product. The enzyme can utilize both NAD+ and NADP+, but the catalytic efficiency with NAD+ is ~5-fold higher. The native electron acceptor of the enzyme is a membrane-bound clostridial-type ferredoxin, which transfers the electrons to an electron-transfer flavoprotein (ETF).
The enzyme appears in viruses and cellular organisms
This bacterial enzyme is involved in degradation of glycine betaine. The enzyme contains non-covalently bound FAD and NAD(P) cofactors, and catalyses the demethylation of both N,N-dimethylglycine and sarcosine, releasing formaldehyde and forming glycine as the final product. The enzyme can utilize both NAD+ and NADP+, but the catalytic efficiency with NAD+ is ~5-fold higher. The native electron acceptor of the enzyme is a membrane-bound clostridial-type ferredoxin, which transfers the electrons to an electron-transfer flavoprotein (ETF).
a mutant strain lacking DgcA accumulates dimethylglycine and glycine betaine when exposed to choline, resulting in hyperinduction of GbdR-regulated transcripts
under high-salinity conditions, the presence of dimethylglycine inhibits growth of the wild type and induces the production and accumulation of trehalose and glucosylglycerate intracellularly. Exogenous addition of dimethylglycine significantly improves the growth rates of DdhC mutants incubated at 37°C synthetic medium with sarcosine as the sole N source
under high-salinity conditions, the presence of dimethylglycine inhibits growth of the wild type and induces the production and accumulation of trehalose and glucosylglycerate intracellularly. Exogenous addition of dimethylglycine significantly improves the growth rates of DdhC mutants incubated at 37°C synthetic medium with sarcosine as the sole N source
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of genes GbcA, DgcA, and SoxA is induced in the presence of choline, glycine betaine, and dimethylglycine. Gene PA5380 is required for this induction
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