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Information on EC 1.5.1.49 - 1-pyrroline-2-carboxylate reductase [NAD(P)H] and Organism(s) Colwellia psychrerythraea and UniProt Accession Q485S0

for references in articles please use BRENDA:EC1.5.1.49
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IUBMB Comments
The enzyme from the bacterium Colwellia psychrerythraea is involved in trans-3-hydroxy-L-proline metabolism. In contrast to EC 1.5.1.1, 1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H], which shows similar activity with 1-piperideine-2-carboxylate and 1-pyrroline-2-carboxylate, this enzyme is specific for the latter. While the enzyme is active with both NADH and NADPH, activity is higher with NADPH.
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Colwellia psychrerythraea
UNIPROT: Q485S0
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The taxonomic range for the selected organisms is: Colwellia psychrerythraea
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
LhpH, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
L-proline:NAD(P)+ 2-oxidoreductase
The enzyme from the bacterium Colwellia psychrerythraea is involved in trans-3-hydroxy-L-proline metabolism. In contrast to EC 1.5.1.1, 1-piperideine-2-carboxylate/1-pyrroline-2-carboxylate reductase [NAD(P)H], which shows similar activity with 1-piperideine-2-carboxylate and 1-pyrroline-2-carboxylate, this enzyme is specific for the latter. While the enzyme is active with both NADH and NADPH, activity is higher with NADPH.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DELTA1-pyrroline-2-carboxylate + NADH + H+
L-proline + NAD+
show the reaction diagram
DELTA1-pyrroline-2-carboxylate + NADPH + H+
L-proline + NADP+
show the reaction diagram
L-proline + NAD+
DELTA1-pyrroline-2-carboxylate + NADH + H+
show the reaction diagram
preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
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-
r
L-proline + NADP+
DELTA1-pyrroline-2-carboxylate + NADPH + H+
show the reaction diagram
preference of the reaction equilibrium to the direction toward NADH dependent reduction. In the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
-
-
r
additional information
?
-
no significant activity with DELTA1-piperideine-2-carboxylate or L-pipecolate. The kcat/Km values for DELTA1-piperideine-2-carboxylate and L-pipecolate in the presence of NADP+(H) are 111fold and 223fold lower than those for DELTA1-pyrroline-2-carboxylate and L-proline, mainly caused by 90fold and 51fold lower kcat values, respectively
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DELTA1-pyrroline-2-carboxylate + NADH + H+
L-proline + NAD+
show the reaction diagram
the enzyme is part of the trans-3-hydroxy-L-proline pathway
-
-
?
DELTA1-pyrroline-2-carboxylate + NADPH + H+
L-proline + NADP+
show the reaction diagram
the enzyme is part of the trans-3-hydroxy-L-proline pathway
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
NADH
the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value
NADP+
in the oxidization of L-proline the ratio of NADP+ to NAD+ in kcat/Km value is about 150:1
NADPH
the preferred cofactor is NADPH, lower activity with NADH. The kcat/Km value for DELTA1-pyrroline-2-carboxylate in the presence of NADH is 5.6fold lower than that in the presence of NADPH, mainly caused by the 12fold lower kcat value
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.79 - 5.9
DELTA1-pyrroline-2-carboxylate
18.3 - 18.8
L-proline
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.35 - 124.5
DELTA1-pyrroline-2-carboxylate
0.0069 - 1.01
L-proline
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7 - 21.1
DELTA1-pyrroline-2-carboxylate
0.000367 - 0.05519
L-proline
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00451
L-proline, pH 10.5, 30°C, cosubstrate: NAD+
0.63
L-proline, pH 10.5, 30°C, cosubstrate: NADP+
30.2
DELTA1-pyrroline-2-carboxylate + NADPH, pH 6.5, 30°C
4.76
DELTA1-pyrroline-2-carboxylate + NADH, pH 6.5, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.5
assay at, oxidation of L-proline
6.5
assay at, reduction of DELTA1-pyrroline-2-carboxylate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is part of the trans-3-hydroxy-L-proline pathway
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Watanabe, S.; Tanimoto, Y.; Yamauchi, S.; Tozawa, Y.; Sawayama, S.; Watanabe, Y.
Identification and characterization of trans-3-hydroxy-L-proline dehydratase and DELTA(1)-pyrroline-2-carboxylate reductase involved in trans-3-hydroxy-L-proline metabolism of bacteria
FEBS Open Bio
4
240-250
2014
Colwellia psychrerythraea (Q485S0)
Manually annotated by BRENDA team