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EC Tree
IUBMB Comments This enzyme, isolated from the bacterium Geobacillus thermodenitrificans, participates in the pathway of tryptophan degradation. The enzyme is part of a system that also includes a bifunctional riboflavin kinase/FMN adenylyltransferase and EC 1.14.14.8, anthranilate 3-monooxygenase (FAD). It can reduce either FAD or flavin mononucleotide (FMN) but prefers FAD. The enzyme has a slight preference for NADPH as acceptor. cf. EC 1.5.1.37, FAD reductase (NADH).
The enzyme appears in viruses and cellular organisms
Synonyms
FAD reductase,
GTNG_3158 ,
more
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FAD reductase
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GTNG_3158
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FADH2 + NAD(P)+ = FAD + NAD(P)H + H+
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FADH2:NAD(P)+ oxidoreductase
This enzyme, isolated from the bacterium Geobacillus thermodenitrificans, participates in the pathway of tryptophan degradation. The enzyme is part of a system that also includes a bifunctional riboflavin kinase/FMN adenylyltransferase and EC 1.14.14.8, anthranilate 3-monooxygenase (FAD). It can reduce either FAD or flavin mononucleotide (FMN) but prefers FAD. The enzyme has a slight preference for NADPH as acceptor. cf. EC 1.5.1.37, FAD reductase (NADH).
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FAD + NADH + H+
FADH2 + NAD+
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95% activity with NADH relative to NADPH
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FAD + NADPH + H+
FADH2 + NADP+
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higher activity is obtained with FAD than with FMN using NADPH as the cofactor
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FMN + NADPH + H+
FMNH2 + NADP+
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higher activity is obtained with FAD than with FMN using NADPH as the cofactor
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additional information
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anthranilate can also be oxidized to 3-hydroxyanthranilate by GTNG_3158 in the presence of FMN and ATP
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55.47
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using FMN and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
58.2
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using FAD and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
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brenda
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NFADR_GEOTN
Geobacillus thermodenitrificans (strain NG80-2)
185
0
20725
Swiss-Prot
-
A0A1X9MAI8_9BACI
177
0
19690
TrEMBL
-
A0A410W8K9_9CORY
188
0
20161
TrEMBL
-
A0A150HAC8_9MICO
219
0
23168
TrEMBL
-
A0A5C5Y5J0_9PLAN
183
0
19628
TrEMBL
-
A0A3G6J161_9CORY
188
0
20375
TrEMBL
-
A0A2P4UFH9_9ACTN
206
0
21117
TrEMBL
-
A0A3G6IUB8_9CORY
188
0
20120
TrEMBL
-
A0A5C6AV99_9BACT
184
0
20051
TrEMBL
-
A0A3N6DAE2_9ACTN
583
0
62779
TrEMBL
-
A0A2S5D5D5_LYSSH
184
0
21353
TrEMBL
-
A0A1E2S3H3_9RHIZ
188
0
19947
TrEMBL
-
A0A0F0L6U2_9MICO
189
0
20364
TrEMBL
-
A0A3N6GB76_9ACTN
223
0
23513
TrEMBL
-
A0A3G4VRB0_9ACTN
217
0
22418
TrEMBL
-
A0A1V9QY51_9LACO
187
0
21138
TrEMBL
-
A0A3E2YI82_9ACTN
207
0
21628
TrEMBL
-
A0A3N6HBR1_9ACTN
219
0
23103
TrEMBL
-
A0A0F0KUV1_9MICO
190
0
20365
TrEMBL
-
A0A3P5WWM9_9RHOB
186
0
19835
TrEMBL
-
A0A7M4DLC5_9MICO
191
0
19441
TrEMBL
-
A0A3N6I4C1_9ACTN
219
0
23124
TrEMBL
-
A0A127MKU8_9PSED
186
0
20224
TrEMBL
-
A0A2K8PRA5_STRLA
220
0
22722
TrEMBL
-
A0A5K6CLW7_ACIB3
Acinetobacter baumannii (strain AB307-0294)
245
0
26585
TrEMBL
-
A0A3N6J5W2_9ACTN
240
0
24987
TrEMBL
-
A0A517VNP9_9PLAN
177
0
19742
TrEMBL
-
A0A6B8VKW0_9CORY
220
0
22777
TrEMBL
-
A0A517T8W1_9PLAN
174
0
19074
TrEMBL
-
A0A0M2HXM1_9MICO
189
0
20218
TrEMBL
-
A0A2K8QTT5_9ACTN
213
0
22788
TrEMBL
-
A0A2K8RH74_9ACTN
213
0
22530
TrEMBL
-
A0A1E7W6U0_9BURK
193
0
21271
TrEMBL
-
A0A150I387_9GAMM
246
0
26587
TrEMBL
-
A0A7X0HTL0_9BACI
184
0
20546
TrEMBL
-
A0A059ZQM3_ACIBA
245
0
26585
TrEMBL
-
A0A0M2H7T4_9MICO
205
0
22148
TrEMBL
-
A0A0F2C9A5_9MICO
205
0
21854
TrEMBL
-
A0A5J6Z9L7_9CORY
213
0
22802
TrEMBL
-
A0A117EB89_9ACTN
202
0
20977
TrEMBL
-
A0A0M2WSV4_9BURK
193
0
21290
TrEMBL
-
A0A4P7QFK8_9CORY
231
0
24409
TrEMBL
-
A0A7W3RCT8_9BACI
185
0
20757
TrEMBL
-
A0A5B9QG42_9BACT
191
0
20698
TrEMBL
-
A0A517WN80_9PLAN
177
0
19800
TrEMBL
-
A0A0C3A4P8_RHOER
188
0
20283
TrEMBL
-
A0A222TNR3_GORRU
232
0
24041
TrEMBL
-
A0A433DQN2_9ACTN
225
0
23862
TrEMBL
-
A0A3N6EP27_9ACTN
216
0
22556
TrEMBL
-
A0A0F0KV86_9MICO
189
0
20137
TrEMBL
-
A0A5C6FCC8_9BACT
172
0
18399
TrEMBL
-
A0A5C6BZQ3_9BACT
174
0
18919
TrEMBL
-
A0A1V5L0J2_9BACT
188
0
20139
TrEMBL
-
A0A5B1CL56_9BACT
174
0
18414
TrEMBL
-
A0A163T1I4_9CELL
201
0
21387
TrEMBL
-
A0A3P5WDS1_9BACL
187
0
21526
TrEMBL
-
A0A1E2S073_9RHIZ
188
0
20562
TrEMBL
-
A0A2U3N252_9GAMM
245
0
26910
TrEMBL
-
A0A517SPL4_9BACT
174
0
18742
TrEMBL
-
A0A5B7UKC7_9ACTN
204
0
21123
TrEMBL
-
A0A3N6DXA0_9ACTN
221
0
23050
TrEMBL
-
A0A399H3N3_9ACTN
213
0
22128
TrEMBL
-
A0A1R7QEB2_ACIJO
211
0
23296
TrEMBL
-
A0A2Z5KBM6_9ACTN
204
0
21295
TrEMBL
-
A0A0M2HFA5_9MICO
212
0
22401
TrEMBL
-
A0A7G6VB15_9BURK
186
0
19806
TrEMBL
-
A0A1R7QBE9_ACIJO
242
0
26387
TrEMBL
-
A0A177HEX9_9ACTN
248
0
25412
TrEMBL
-
A0A1D8AYM2_9BACT
174
0
18734
TrEMBL
-
A0A1V5M2C5_9BACT
172
0
18856
TrEMBL
-
A0A5C5XNH4_9PLAN
175
0
19203
TrEMBL
-
A0A150HXN4_9GAMM
246
0
26595
TrEMBL
-
A0A447IM75_9RHOB
185
0
19190
TrEMBL
-
A0A3P5WP26_9BACL
188
0
21420
TrEMBL
-
A0A2J7YIH0_PSEPU
186
0
20419
TrEMBL
-
A0A517QZR0_9BACT
175
0
18792
TrEMBL
-
A0A1V5M9N9_9BACT
186
0
20355
TrEMBL
-
A0A100JXQ7_STRSC
199
0
20362
TrEMBL
-
A0A5B9QU95_9BACT
181
0
19516
TrEMBL
-
A0A2K9J4X2_9BACI
179
0
20190
TrEMBL
-
A0A1B9EKR7_9ACTN
219
0
23120
TrEMBL
-
A0A517YV42_9BACT
204
0
22408
TrEMBL
-
A0A2S6QZL5_9PROT
192
0
20495
TrEMBL
-
A0A518I1W4_9BACT
175
0
18753
TrEMBL
-
A0A0B0HZ83_9BACL
178
0
19963
TrEMBL
-
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chelating Sepharose column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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Liu, X.; Dong, Y.; Li, X.; Ren, Y.; Li, Y.; Wang, W.; Wang, L.; Feng, L.
Characterization of the anthranilate degradation pathway in Geobacillus thermodenitrificans NG80-2
Microbiology
156
589-595
2010
Geobacillus thermodenitrificans, Geobacillus thermodenitrificans NG80-2
brenda
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