Information on EC 1.5.1.45 - FAD reductase [NAD(P)H]

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The expected taxonomic range for this enzyme is: Geobacillus thermodenitrificans

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EC NUMBER
COMMENTARY hide
1.5.1.45
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RECOMMENDED NAME
GeneOntology No.
FAD reductase [NAD(P)H]
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
FADH2 + NAD(P)+ = FAD + NAD(P)H + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Riboflavin metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
FADH2:NAD(P)+ oxidoreductase
This enzyme, isolated from the bacterium Geobacillus thermodenitrificans, participates in the pathway of tryptophan degradation. The enzyme is part of a system that also includes a bifunctional riboflavin kinase/FMN adenylyltransferase and EC 1.14.14.8, anthranilate 3-monooxygenase (FAD). It can reduce either FAD or flavin mononucleotide (FMN) but prefers FAD. The enzyme has a slight preference for NADPH as acceptor. cf. EC 1.5.1.37, FAD reductase (NADH).
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
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LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
FAD + NADH + H+
FADH2 + NAD+
show the reaction diagram
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95% activity with NADH relative to NADPH
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?
FAD + NADPH + H+
FADH2 + NADP+
show the reaction diagram
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higher activity is obtained with FAD than with FMN using NADPH as the cofactor
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?
FMN + NADPH + H+
FMNH2 + NADP+
show the reaction diagram
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higher activity is obtained with FAD than with FMN using NADPH as the cofactor
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additional information
?
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anthranilate can also be oxidized to 3-hydroxyanthranilate by GTNG_3158 in the presence of FMN and ATP
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55.47
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using FMN and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
58.2
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using FAD and NADPH as substrates, in 20 mM glycine/NaOH (pH 9.0), at 60°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
chelating Sepharose column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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LINKS TO OTHER DATABASES (specific for EC-Number 1.5.1.45)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)