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2 biopterin + 3 NADPH + 3 H+
dihydrobiopterin + tetrahydrobiopterin + 3 NADP+
6-biopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
7,8-dihydrofolate + NADPH + H+
5,6,7,8-tetrahydrofolate + NADP+
7,8-dihydromonapterin + NADPH + H+
5,6,7,8-tetrahydromonapterin + NADP+
7,8-dihydroneopterin + NADPH + H+
5,6,7,8-tetrahydroneopterin + NADP+
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
biopterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
biopterin + NADPH + H+
dihydrobiopterin + NADP+
dihydrobiopterin + NADPH
5,6,7,8-tetrahydrobiopterin + NADP+
Dihydrobiopterin + NADPH
?
dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
Dihydrofolate + NADPH
5,6,7,8-Tetrahydrofolate + NADP+
Dihydroneopterin + NADPH
?
dihydrosepiapterin + NADPH
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
folate + NADPH + H+
7,8-dihydrofolate + NADP+
folate + NADPH + H+
7,8-dihydrofolate + tetrahydrofolate + NADP+
quinonoid 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
?
quinonoid dihydrobiopterin + NADPH + H+
tetrahydrobiopterin + NADP+
additional information
?
-
2 biopterin + 3 NADPH + 3 H+

dihydrobiopterin + tetrahydrobiopterin + 3 NADP+
essential enzyme of pterin and folate metabolism
-
-
?
2 biopterin + 3 NADPH + 3 H+
dihydrobiopterin + tetrahydrobiopterin + 3 NADP+
-
salvage of pterins, enzyme acts as a metabolic bypass for drugs targeting dihydrofolate reductase, PTR1 contributes about 10% of the reduction of folates in wild-type cells while the remaining 90% is due to the activity of dihydrofolate reductase-thymidylate synthase (EC 1.5.1.3)
-
-
?
6-biopterin + NADPH + H+

5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
6-biopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+

5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
low activity
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrofolate + NADPH + H+

5,6,7,8-tetrahydrofolate + NADP+
low activity
-
-
?
7,8-dihydrofolate + NADPH + H+
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
?
7,8-dihydrofolate + NADPH + H+
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
?
7,8-dihydromonapterin + NADPH + H+

5,6,7,8-tetrahydromonapterin + NADP+
cf. EC 1.5.1.50, moderate activity
-
-
?
7,8-dihydromonapterin + NADPH + H+
5,6,7,8-tetrahydromonapterin + NADP+
cf. EC 1.5.1.50, moderate activity
-
-
?
7,8-dihydromonapterin + NADPH + H+
5,6,7,8-tetrahydromonapterin + NADP+
cf. EC 1.5.1.50, moderate activity
-
-
?
7,8-dihydroneopterin + NADPH + H+

5,6,7,8-tetrahydroneopterin + NADP+
stereoisomer of biopterin, moderate activity
-
-
?
7,8-dihydroneopterin + NADPH + H+
5,6,7,8-tetrahydroneopterin + NADP+
stereoisomer of biopterin, moderate activity
-
-
?
7,8-dihydroneopterin + NADPH + H+
5,6,7,8-tetrahydroneopterin + NADP+
stereoisomer of biopterin, moderate activity
-
-
?
biopterin + 2 NADPH + 2 H+

5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
-
-
?
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
-
substrate binding structure
-
-
?
Biopterin + NADPH

5,6,7,8-Tetrahydrobiopterin + NADP+
-
-
-
?
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
-
-
-
-
?
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
-
activity with NADH is less than 5% of the activity with NADPH
-
-
?
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
-
-
-
-
?
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
2-step reaction via intermediate 7,8-dihydrobiopterin
-
-
?
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
-
-
-
-
?
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
-
no activity with NADH and NADP+
-
-
?
Biopterin + NADPH
5,6,7,8-Tetrahydrobiopterin + NADP+
-
no activity with NADH and NADP+
-
-
?
biopterin + NADPH + H+

7,8-dihydrobiopterin + NADP+
-
-
-
?
biopterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
best substrate
-
-
?
biopterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
-
-
-
?
biopterin + NADPH + H+

dihydrobiopterin + NADP+
-
-
-
-
?
biopterin + NADPH + H+
dihydrobiopterin + NADP+
-
-
-
?
biopterin + NADPH + H+
dihydrobiopterin + NADP+
-
-
-
-
?
biopterin + NADPH + H+
dihydrobiopterin + NADP+
-
-
-
?
dihydrobiopterin + NADPH

5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
-
?
dihydrobiopterin + NADPH
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
r
Dihydrobiopterin + NADPH

?
-
-
-
-
?
Dihydrobiopterin + NADPH
?
-
-
-
-
?
Dihydrobiopterin + NADPH
?
-
-
-
-
?
Dihydrobiopterin + NADPH
?
-
-
-
-
?
dihydrobiopterin + NADPH + H+

5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
-
?
dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
-
?
dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
Dihydrofolate + NADPH

5,6,7,8-Tetrahydrofolate + NADP+
-
-
-
-
?
Dihydrofolate + NADPH
5,6,7,8-Tetrahydrofolate + NADP+
-
-
-
?
Dihydrofolate + NADPH
5,6,7,8-Tetrahydrofolate + NADP+
-
-
-
-
?
Dihydrofolate + NADPH
5,6,7,8-Tetrahydrofolate + NADP+
-
-
-
-
?
Dihydrofolate + NADPH
5,6,7,8-Tetrahydrofolate + NADP+
-
-
-
-
?
Dihydroneopterin + NADPH

?
-
-
-
-
?
Dihydroneopterin + NADPH
?
-
-
-
-
?
Dihydroneopterin + NADPH
?
-
no activity
-
-
?
dihydrosepiapterin + NADPH

?
-
-
-
-
?
dihydrosepiapterin + NADPH
?
-
-
-
-
?
dihydrosepiapterin + NADPH
?
-
no activity
-
-
?
folate + NADPH

5,6,7,8-tetrahydrofolate + NADP+
-
-
-
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
-
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
-
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
-
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
-
?
folate + NADPH
5,6,7,8-tetrahydrofolate + NADP+
-
-
-
-
?
folate + NADPH + H+

7,8-dihydrofolate + NADP+
high activity, see also EC 1.5.1.3
-
-
?
folate + NADPH + H+
7,8-dihydrofolate + NADP+
reaction of EC 1.5.1.3
-
-
?
folate + NADPH + H+
7,8-dihydrofolate + NADP+
-
-
-
?
folate + NADPH + H+

7,8-dihydrofolate + tetrahydrofolate + NADP+
-
-
-
-
?
folate + NADPH + H+
7,8-dihydrofolate + tetrahydrofolate + NADP+
essential enzyme of pterin and folate metabolism
-
-
?
quinonoid 6,7-dimethyl-7,8-dihydropterin + NADPH + H+

?
-
-
-
-
?
quinonoid 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
?
-
-
-
-
?
quinonoid dihydrobiopterin + NADPH + H+

tetrahydrobiopterin + NADP+
-
-
-
-
?
quinonoid dihydrobiopterin + NADPH + H+
tetrahydrobiopterin + NADP+
-
-
-
-
?
additional information

?
-
enzyme PruA does not exhibit pteridine reductase activity with 7,8-dihydrofolate or fully oxidized pterins. PruA exhibits maximal catalytic efficiency with H2BPt, while both H2MPt and 7,8-dihydroneopterin (H2NPt) also serve as competent substrates. Substrate specificity, overview. No activity with 7,8-dihydrofolate, biopterin, and folate
-
-
-
additional information
?
-
enzyme PruA does not exhibit pteridine reductase activity with 7,8-dihydrofolate or fully oxidized pterins. PruA exhibits maximal catalytic efficiency with H2BPt, while both H2MPt and 7,8-dihydroneopterin (H2NPt) also serve as competent substrates. Substrate specificity, overview. No activity with 7,8-dihydrofolate, biopterin, and folate
-
-
-
additional information
?
-
enzyme PruA does not exhibit pteridine reductase activity with 7,8-dihydrofolate or fully oxidized pterins. PruA exhibits maximal catalytic efficiency with H2BPt, while both H2MPt and 7,8-dihydroneopterin (H2NPt) also serve as competent substrates. Substrate specificity, overview. No activity with 7,8-dihydrofolate, biopterin, and folate
-
-
-
additional information
?
-
enzyme is associated with folate metabolism, responsible for salvage of pterins and reduction of folates
-
-
?
additional information
?
-
-
enzyme is associated with folate metabolism, responsible for salvage of pterins and reduction of folates
-
-
?
additional information
?
-
NADPH binds first, the enzyme-NADPH complex binds the substrate
-
-
?
additional information
?
-
-
NADPH binds first, the enzyme-NADPH complex binds the substrate
-
-
?
additional information
?
-
substrate specificity, overview
-
-
-
additional information
?
-
-
no activity with quinoid dihydrobiopterin
-
-
?
additional information
?
-
-
primary enzyme mediating pteridine salvage
-
-
?
additional information
?
-
-
the enzyme mediates the synthesis of tetrahydropteridines
-
-
?
additional information
?
-
essential for the salvage of pterins
-
-
?
additional information
?
-
-
essential for the salvage of pterins
-
-
?
additional information
?
-
-
thermal shift enzyme assay optimization
-
-
?
additional information
?
-
enzyme PTR1 synthesizes the formation of tetrahydrobiopterin from biopterin via dihydrobiopterin or from the quinoide form dihydrobiopterin, pathway overview
-
-
-
additional information
?
-
-
enzyme PTR1 synthesizes the formation of tetrahydrobiopterin from biopterin via dihydrobiopterin or from the quinoide form dihydrobiopterin, pathway overview
-
-
-
additional information
?
-
7,8-dihydro-L-biopterin and pyrimethamine (PMA) interact with enzyme residues S112, Y194, L226, and S227, molecular dynamics and docking study, overview
-
-
-
additional information
?
-
-
7,8-dihydro-L-biopterin and pyrimethamine (PMA) interact with enzyme residues S112, Y194, L226, and S227, molecular dynamics and docking study, overview
-
-
-
additional information
?
-
-
the enzyme mediates the synthesis of tetrahydropteridines
-
-
?
additional information
?
-
enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway
-
-
?
additional information
?
-
-
enzyme is used by the organism to bypass antifolate inhibition, catalytic pathway
-
-
?
additional information
?
-
enzyme PTR1 synthesizes the formation of tetrahydrobiopterin from biopterin via dihydrobiopterin or from the quinoide form dihydrobiopterin, pathway overview
-
-
-
additional information
?
-
-
the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates
-
-
?
additional information
?
-
-
essential for the salvage of pterins
-
-
?
additional information
?
-
-
the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2 biopterin + 3 NADPH + 3 H+
dihydrobiopterin + tetrahydrobiopterin + 3 NADP+
6-biopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
7,8-dihydrofolate + NADPH + H+
5,6,7,8-tetrahydrofolate + NADP+
biopterin + 2 NADPH + 2 H+
5,6,7,8-tetrahydrobiopterin + 2 NADP+
biopterin + NADPH + H+
7,8-dihydrobiopterin + NADP+
biopterin + NADPH + H+
dihydrobiopterin + NADP+
dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
folate + NADPH + H+
7,8-dihydrofolate + NADP+
folate + NADPH + H+
7,8-dihydrofolate + tetrahydrofolate + NADP+
additional information
?
-
2 biopterin + 3 NADPH + 3 H+

dihydrobiopterin + tetrahydrobiopterin + 3 NADP+
essential enzyme of pterin and folate metabolism
-
-
?
2 biopterin + 3 NADPH + 3 H+
dihydrobiopterin + tetrahydrobiopterin + 3 NADP+
-
salvage of pterins, enzyme acts as a metabolic bypass for drugs targeting dihydrofolate reductase, PTR1 contributes about 10% of the reduction of folates in wild-type cells while the remaining 90% is due to the activity of dihydrofolate reductase-thymidylate synthase (EC 1.5.1.3)
-
-
?
6-biopterin + NADPH + H+

5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
6-biopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+

5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
best substrate
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+
-
-
-
?
7,8-dihydrobiopterin + NADPH + H+
5,6,7,8-tetrahydrobiopterin + NADP+