Information on EC 1.5.1.16 - D-lysopine dehydrogenase

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The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
1.5.1.16
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RECOMMENDED NAME
GeneOntology No.
D-lysopine dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O = L-lysine + pyruvate + NADPH + H+
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
-
-
-
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reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Lysine degradation
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-
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SYSTEMATIC NAME
IUBMB Comments
N2-(D-1-carboxyethyl)-L-lysine:NADP+ oxidoreductase (L-lysine-forming)
In the reverse reaction, a number of L-amino acids can act instead of L-lysine, and 2-oxobutanoate and, to a lesser extent, glyoxylate can act instead of pyruvate.
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CAS REGISTRY NUMBER
COMMENTARY hide
65187-41-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
marine mussel
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-
Manually annotated by BRENDA team
no activity in Brugmansia sanguinea
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
show the reaction diagram
N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O
L-ornithine + pyruvate + NADPH
show the reaction diagram
additional information
?
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-
no substrate: L-citrulline
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
show the reaction diagram
-
-
-
-
r
N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O
L-ornithine + pyruvate + NADPH
show the reaction diagram
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-
-
r
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.8
canavanine
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-
0.16
D(+)-lysopine
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-
0.8
D(+)-octopine
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-
1.1 - 4.4
L-arginine
17.1
L-citrulline
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-
6.1
L-glutamine
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-
6.1
L-histidine
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-
0.56 - 1.8
L-lysine
1.3
L-methionine
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-
2.3 - 6
L-ornithine
0.01
NADP+
-
-
0.003
NADPH
-
-
0.23
pyruvate
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
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L-arginine
6.3
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L-methionine
6.7
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L-ornithine
8.9
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lysopine
9.1
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N2-(D-1-carboxyethyl)-L-ornithine i.e. octopine
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39000
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SDS-PAGE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, many weeks
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4°C, some days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homogeneity
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internode segments infected with Agrobacterium tumefaciens strain B6S3
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leaf tumors initiated by infection with Agrobacterium tumefaciens, partial
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA fragment encodes for a fusion protein of chloramphenicol acetyltransferase and a part of lysopine dehydrogenase
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LINKS TO OTHER DATABASES (specific for EC-Number 1.5.1.16)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)