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EC Tree
IUBMB Comments In the reverse reaction, a number of L-amino acids can act instead of L-lysine, and 2-oxobutanoate and, to a lesser extent, glyoxylate can act instead of pyruvate.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
D(+)-lysopine dehydrogenase, D-lysopine synthase, dehydrogenase, D-lysopine, lysopine dehydrogenase, Lysopine synthase,
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D(+)-lysopine dehydrogenase
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D-lysopine synthase
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dehydrogenase, D-lysopine
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lysopine dehydrogenase
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Lysopine synthase
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O = L-lysine + pyruvate + NADPH + H+
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N2-(D-1-carboxyethyl)-L-lysine:NADP+ oxidoreductase (L-lysine-forming)
In the reverse reaction, a number of L-amino acids can act instead of L-lysine, and 2-oxobutanoate and, to a lesser extent, glyoxylate can act instead of pyruvate.
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D-(+)-octopine + pyruvate + NADH + H+
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r
L-arginine + pyruvate + NADH + H+
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L-canavanine + pyruvate + NADH + H+
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L-citrulline + pyruvate + NADH + H+
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L-glutamine + pyruvate + NADH + H+
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L-histidine + pyruvate + NADH + H+
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L-methionine + pyruvate + NADH + H+
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O
L-ornithine + pyruvate + NADPH
additional information
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no substrate: L-citrulline
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L-arginine + pyruvate + NADH + H+
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L-arginine + pyruvate + NADH + H+
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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cells transformed with AB-3, AT-6 or Hm1 mainly produce lysopine and octopine from L-lysine or L-arginine, cells transformed with B6S3 convert L-methionine, L-arginine, L-histidine, L-lysine into the corresponding opines
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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enzyme also utilizes L-arginine
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine is identical with lysopine, in the reverse reaction L-methionine, L-ornithine, L-arginine, L-histidine, L-glutamine can act instead of L-lysine
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N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O
L-ornithine + pyruvate + NADPH
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enzyme also utilizes canavanine instead of L-ornithine
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N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O
L-ornithine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-lysine + NADP+ + H2O
L-lysine + pyruvate + NADPH
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N2-(D-1-carboxyethyl)-L-ornithine + NADP+ + H2O
L-ornithine + pyruvate + NADPH
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r
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NADH
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Neoplasms
A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities.
Neoplasms
Differential expression of crown gall tumor markers in transformants obtained after in vitro Agrobacterium tumefaciens-induced transformation of cell wall regenerating protoplasts derived from Nicotiana tabacum.
Neoplasms
Expression of plant tumor-specific proteins in minicells of Escherichia coli: a fusion protein of lysopine dehydrogenase with chloramphenicol acetyltransferase.
Neoplasms
Properties of D(+)-lysopine dehydrogenase from crown gall tumour tissue.
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1.1
L-arginine
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0.56
L-lysine
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2.3
L-ornithine
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9.1
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N2-(D-1-carboxyethyl)-L-ornithine i.e. octopine
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brenda
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brenda
marine mussel
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brenda
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no activity in Brugmansia sanguinea
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brenda
internode segments infected with Agrobacterium tumefaciens strain B6S3
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brenda
tumors induced by Agrobacterium tumefaciens strains: AB-3, AT-6, Hm-1
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brenda
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leaf tumors initiated by infection with Agrobacterium tumefaciens, strain B6S3
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tobacco crown gall line A6-S1
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brenda
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induced in stem segments of Kalanchoe daigremontiana with Agrobacterium tumefaciens strains AB-3, AT-6, Hm-1
brenda
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internode segments infected with Agrobacterium tumefaciens strain B6S3
brenda
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brenda
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leaves infected with Agrobacterium tumefaciens strain B6S3
brenda
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DHLO_AGRT4
358
0
38797
Swiss-Prot
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DHLO_AGRT9
358
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38797
Swiss-Prot
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DHLO_AGRVI
358
0
38860
Swiss-Prot
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internode segments infected with Agrobacterium tumefaciens strain B6S3
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leaf tumors initiated by infection with Agrobacterium tumefaciens, partial
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DNA fragment encodes for a fusion protein of chloramphenicol acetyltransferase and a part of lysopine dehydrogenase
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Coughlan, M.; O'Carra, P.
The lysopine and octopine dehydrogenase activities of Mytilus edulis are catalyzed by a single enzyme
Biochem. Soc. Trans.
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128S
1996
Mytilus edulis
brenda
Otten, L.A.B.M.; Vreugdenhil, D.; Schilperoort, R.A.
Properties of D(+)-lysopine dehydrogenase from crown gall tumour tissue
Biochim. Biophys. Acta
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268-277
1977
Nicotiana tabacum
brenda
Otten, L.
Lysopine dehydrogenase activity as an early marker in crown gall transformation
Plant Sci. Lett.
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15-27
1982
Datura inoxia, Helianthus annuus, Bryophyllum daigremontianum, Nicotiana glauca x Nicotiana langsdorffii, Nicotiana tabacum, no activity in Brugmansia sanguinea
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brenda
Otten, L.; Szegedi, E.
Crown galls induced by octopine-degrading biotype 3 strains of Agrobacterium tumefaciens contain a new form of lysopine dehydrogenase
Plant Sci.
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81-85
1985
Bryophyllum daigremontianum
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brenda
Schröder, J.; Hillebrand, A.; Klipp, W.; Puhler, A.
Expression of plant tumor-specific proteins in minicells of Escherichia coli: a fusion protein of lysopine dehydrogenase with chloramphenicol acetyltransferase
Nucleic Acids Res.
9
5187-5202
1981
Nicotiana tabacum
brenda
Schröder, J.; Schröder, G.; Huisman, H.; Schilperoort, R.A.; Schell, J.
The mRNA for lysopine dehydrogenase in plant tumor cells is complementary to a Ti-plasmid fragment
FEBS Lett.
129
166-168
1981
Nicotiana tabacum
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brenda
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1.5.1.16 D-lysopine dehydrogenase
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