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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH + H+
5,10-methylenetetrahydrofolate + NADP+
5,10-methenyltetrahydrofolate + NADPH + H+
5,10-methylenetetrahydropteroylglutamate + NAD+
5,10-methenyltetrahydropteroylglutamate + NADH + H+
5,10-methylenetetrahydropteroylglutamate + NADP+
5,10-methenyltetrahydropteroylglutamate + NADPH + H+
5,10-methylenetetrahydropteroylpentaglutamate + NAD+
5,10-methenyltetrahydropteroylpentaglutamate + NADH + H+
5,10-methylenetetrahydropteroylpentaglutamate + NADP+
5,10-methenyltetrahydropteroylpentaglutamate + NADPH + H+
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
additional information
?
-
5,10-methylenetetrahydrofolate + NAD+

5,10-methenyltetrahydrofolate + NADH
-
-
-
?
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH
-
-
-
-
?
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH
-
-
-
?
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH
enzyme provides one-carbon units for purine synthesis during embryogenesis, metabolism overview
-
-
?
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH
N-terminal catalytic domain
-
-
?
5,10-methylenetetrahydrofolate + NAD+

5,10-methenyltetrahydrofolate + NADH + H+
the bifunctional enzyme also shows N5,N10-methenyltetrahydrofolate cyclohydrolase activity EC 3.5.4.9. with NADP+ (1 mM), the enzyme shows less than 0.1% of the activity obtained with NAD+ (1 mM). The purified enzyme shows no activity with methylene-tetrahydromethanopterin or with 5,10-methylenetetrahydrosarcinapterin either in the presence of NAD+ or NADP+ or in the presence of F420
-
-
?
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH + H+
the bifunctional enzyme also shows N5,N10-methenyltetrahydrofolate cyclohydrolase activity EC 3.5.4.9. with NADP+ (1 mM), the enzyme shows less than 0.1% of the activity obtained with NAD+ (1 mM). The purified enzyme shows no activity with methylene-tetrahydromethanopterin or with 5,10-methylenetetrahydrosarcinapterin either in the presence of NAD+ or NADP+ or in the presence of F420
-
-
?
5,10-methylenetetrahydrofolate + NADP+

5,10-methenyltetrahydrofolate + NADPH + H+
-
-
-
?
5,10-methylenetetrahydrofolate + NADP+
5,10-methenyltetrahydrofolate + NADPH + H+
-
?
-
-
5,10-methylenetetrahydrofolate + NADP+
5,10-methenyltetrahydrofolate + NADPH + H+
about 15% of the rate with NAD+
-
-
?
5,10-methylenetetrahydropteroylglutamate + NAD+

5,10-methenyltetrahydropteroylglutamate + NADH + H+
NAD+-dependent dehydrogenase activity of MTHFD2 is 8.5fold higher than its NADP+-dependent activity
-
-
?
5,10-methylenetetrahydropteroylglutamate + NAD+
5,10-methenyltetrahydropteroylglutamate + NADH + H+
NAD+-dependent dehydrogenase activity of MTHFD2L is 3.4fold higher than its NADP+-dependent activity under saturating substrate conditions
-
-
?
5,10-methylenetetrahydropteroylglutamate + NADP+

5,10-methenyltetrahydropteroylglutamate + NADPH + H+
NAD+-dependent dehydrogenase activity of MTHFD2 is 8.5fold higher than its NADP+-dependent activity
-
-
?
5,10-methylenetetrahydropteroylglutamate + NADP+
5,10-methenyltetrahydropteroylglutamate + NADPH + H+
NAD+-dependent dehydrogenase activity of MTHFD2L is 3.4fold higher than its NADP+-dependent activity under saturating substrate conditions
-
-
?
5,10-methylenetetrahydropteroylpentaglutamate + NAD+

5,10-methenyltetrahydropteroylpentaglutamate + NADH + H+
NAD+-dependent dehydrogenase activity of MTHFD2 with the substrate 5,10-methylenetetrahydropteroylglutamate is 8.5fold higher than its NADP+-dependent activity. While the NAD+-dependent activity of MTHFD2 slightly decreases, its maximal NADP+-dependent activity considerably increases with 5,10-methylenetetrahydropteroylpentaglutamate compared to 5,10-methylenetetrahydropteroylglutamate
-
-
?
5,10-methylenetetrahydropteroylpentaglutamate + NAD+
5,10-methenyltetrahydropteroylpentaglutamate + NADH + H+
NAD+-dependent dehydrogenase activity of MTHFD2L with the substrate 5,10-methylenetetrahydropteroylglutamate is 3.4fold higher than its NADP+-dependent activity under saturating substrate conditions. While the NAD+-dependent activity of MTHFD2 slightly decreases, its maximal NADP+-dependent activity considerably increases with 5,10-methylenetetrahydropteroylpentaglutamate compared to 5,10-methylenetetrahydropteroylglutamate
-
-
?
5,10-methylenetetrahydropteroylpentaglutamate + NADP+

5,10-methenyltetrahydropteroylpentaglutamate + NADPH + H+
NAD+-dependent dehydrogenase activity of MTHFD2 with the substrate 5,10-methylenetetrahydropteroylglutamate is 8.5fold higher than its NADP+-dependent activity. While the NAD+-dependent activity of MTHFD2 slightly decreases, its maximal NADP+-dependent activity considerably increases with 5,10-methylenetetrahydropteroylpentaglutamate compared to 5,10-methylenetetrahydropteroylglutamate
-
-
?
5,10-methylenetetrahydropteroylpentaglutamate + NADP+
5,10-methenyltetrahydropteroylpentaglutamate + NADPH + H+
NAD+-dependent dehydrogenase activity of MTHFD2L with the substrate 5,10-methylenetetrahydropteroylglutamate is 3.4fold higher than its NADP+-dependent activity under saturating substrate conditions. While the NAD+-dependent activity of MTHFD2 slightly decreases, its maximal NADP+-dependent activity considerably increases with 5,10-methylenetetrahydropteroylpentaglutamate compared to 5,10-methylenetetrahydropteroylglutamate
-
-
?
L-5,10-methenyl-tetrahydrofolate + NADH

L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
Antheraea eucalypti
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
392077, 392079, 392083, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392093 -
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
392075, 392076, 392077, 392078, 392079, 392080, 392081, 392082, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392092, 392093 -
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+

5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
Antheraea eucalypti
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
392077, 392079, 392083, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392093 -
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
392075, 392076, 392077, 392078, 392079, 392080, 392081, 392082, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392092, 392093 -
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
additional information

?
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-
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?
additional information
?
-
-
active only with L-5,10-methylenetetrahydrofolate, the L-isomer does not affect the reaction
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-
?
additional information
?
-
-
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?
additional information
?
-
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?
additional information
?
-
-
bifunctional enzyme
-
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?
additional information
?
-
-
key enzyme in folate metabolism playing a major role in provision of methyl groups for DNA methylation and in production of dTMP for DNA synthesis
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
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?
additional information
?
-
-
level of enzyme activity appears to be strain dependent with Swiss mice having the highest activity
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?
additional information
?
-
enzyme deficiency results in a number of diseases
-
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?
additional information
?
-
-
enzyme deficiency results in a number of diseases
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
contains 2 enzymes, monofunctional NAD+ dependent dehydrogenase EC1.5.1.15 and trifunctional NADP+ dependent dehydrogenase, EC1.5.1.5
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-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH
5,10-methylenetetrahydrofolate + NADP+
5,10-methenyltetrahydrofolate + NADPH + H+
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
additional information
?
-
5,10-methylenetetrahydrofolate + NAD+

5,10-methenyltetrahydrofolate + NADH
-
-
-
-
?
5,10-methylenetetrahydrofolate + NAD+
5,10-methenyltetrahydrofolate + NADH
enzyme provides one-carbon units for purine synthesis during embryogenesis, metabolism overview
-
-
?
5,10-methylenetetrahydrofolate + NADP+

5,10-methenyltetrahydrofolate + NADPH + H+
-
-
-
?
5,10-methylenetetrahydrofolate + NADP+
5,10-methenyltetrahydrofolate + NADPH + H+
-
?
-
-
L-5,10-methenyl-tetrahydrofolate + NADH

L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
Antheraea eucalypti
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
physiological direction in most organisms is the forward rection, in the acetogens the reverse reaction is the physiologically important one
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
392077, 392079, 392083, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392093 -
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
392075, 392076, 392077, 392078, 392079, 392080, 392081, 392082, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392092, 392093 -
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methenyl-tetrahydrofolate + NADH
L-5,10-methylenetetrahydrofolate + NAD+
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+

5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
Antheraea eucalypti
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
392077, 392079, 392083, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392093 -
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
392075, 392076, 392077, 392078, 392079, 392080, 392081, 392082, 392084, 392085, 392086, 392087, 392088, 392090, 392091, 392092, 392093 -
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
L-5,10-methylenetetrahydrofolate + NAD+
5,10-methenyl-tetrahydrofolate + NADH
-
-
-
-
r
additional information

?
-
-
key enzyme in folate metabolism playing a major role in provision of methyl groups for DNA methylation and in production of dTMP for DNA synthesis
-
-
?
additional information
?
-
enzyme deficiency results in a number of diseases
-
-
?
additional information
?
-
-
enzyme deficiency results in a number of diseases
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0001
-
C-57/Bl, bone marrow and whole adrenal, CD-1, adrenal
0.0006
-
RLE cells, virus 3611.MSV v-raf oncogene-transformed
0.00076
-
fibroblasts, syrian hamster embryo
0.0011
-
Swiss, bone marrow
0.0017
-
cell line B-16 solid tumor
0.002
-
RLE cells, virus pRNR16 v-Ha-ras oncogene-transformed
0.0021
-
RLE cells, virus J2 v-raf/v-myc oncogene-transformed
0.0024
-
cell line M4, human cutaneous melanoma
0.0027
-
cell line MCF-7, 100% confluent
0.003
-
cell line LR-73, chinese hamster ovary
0.0031
-
cell line K562, human chronic myeolcytic leukemia
0.0033
-
cell line TL3, EBV-transformed
0.0035
-
cell line MCF-7, 50% confluent
0.004
-
cell line CCRF-CEM
0.0046
-
cell line TL4, EBV-transformed
0.0047
-
cell line MG-63, human osteosarcoma
0.0049
-
cell line BeWo, human choriocarcinoma
0.0059
-
cell line P815, ascites in vivo
0.0066
-
cell line EL4, ascites in vivo
0.0068
-
L-cells, murine fibroblast
0.0069
-
cell line MCF-7, human breast carcinoma
0.0071
-
cell line P-388 leukemia ip
0.0078
-
cell line CCRF-CEM, human leukemia
0.0089
-
Ehrlich ascites, ascites in vivo
0.0094
-
cell line EL4, murine T-cell lymphoma
0.0097
-
cell line YAC, murine lymphoma (Moloney virus-induced)
0.0105
-
cell line 3T3-SV40, SV40 transformed
0.02
-
Ehrlich ascites, murine mastocytoma
0.024
mutant D190E, cosubstrate NAD+, pH 7.3
0.156
mutant R198A, cosubstrate NADP+, pH 7.3
0.16
mutant D133A, cosubstrate NADP+, pH 7.3
0.17
mutant R198A, cosubstrate NAD+, pH 7.3
0.207
mutant R198S, cosubstrate NAD+, pH 7.3
0.392
mutant D133A, cosubstrate NAD+, pH 7.3
1 - 2
pH 6.0, 37ưC, purified enzyme, recombinant
1.01
mutant D133S, cosubstrate NADP+, pH 7.3
1.24
mutant R198K, cosubstrate NADP+, pH 7.3
1.54
mutant R198S, cosubstrate NADP+, pH 7.3
12.6
mutant R198K, cosubstrate NAD+, pH 7.3
12.9
-
embryonal carcinoma
2 - 2.5
wild-type, cosubstrate NAD+, pH 7.3
2.92
wild-type, cosubstrate NADP+, pH 7.3
3.64
mutant D190N, cosubstrate NAD+, pH 7.3
3.96
mutant D190N, cosubstrate NADP+, pH 7.3
3.99
mutant D133S, cosubstrate NAD+, pH 7.3
4.6
mutant D133N, cosubstrate NAD+, pH 7.3
5
pH 6.0, 37ưC, extract from Escherichia coli cells carrying the expression vector
0.0002

-
adrenal cortex
0.0003

-
cell line MRC-5
0.0003
-
HEP-G2 tumor in nude mice
0.0003
-
RLE cells and RLE cells virus J5 v-myc oncogene-transformed
0.0004

-
spleen
0.0004
-
CD-1, bone marrow
0.0005

-
human liver tumor
0.0005
-
colon 38 solid tumor
0.0012

-
cell line Hl-60
0.0012
-
RLE cells, spontaneous oncogene transformed
0.0015

-
cell line HEP-2
0.0015
-
RLE cells, aflatoxin oncogene-transformed
0.0039

-
cell line 3T3, mouse embryo
0.0039
-
cell line P-388 in vitro
0.0042

-
hamster embryo fibroblast, Polyoma virus transformed
0.0042
-
cell line HCT-8R, human intestinal carcinoma
0.0042
-
L-cells, Reovirus infected
0.005

-
cell line TR-3, transformed
0.005
-
cell line Raji, Burkitt lymphoma
0.0055

-
cell line MNNG/HOS, human osteosarcoma
0.0055
-
cell line P815, murine mastocytoma
0.0063

-
cell line MOLT-4
0.0063
-
cell line B-16 in vitro
2.4

-
cutaneous melanoma
2.4
mutant D133N, cosubstrate NADP+, pH 7.3
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Moore, M.R.; O'Brien, W.E.; Ljungdahl, L.G.
Purification and characterisation of nicotinamide adenine dinucleotide-dependent methylenetetrahydrofolate dehydrogenase from Clostridium formicoaceticum
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5250-5253
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Saccharomyces cerevisiae, Gottschalkia acidurici, Clostridium formicaceticum, Mus musculus
brenda
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Purification and properties of NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Acetobacterium woodii
J. Biol. Chem.
259
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1984
Acetobacterium woodii, Saccharomyces cerevisiae, Clostridium formicaceticum, Mus musculus
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Mejia, N.; Mackenzie, R.E.
NAD-dependent methylenetetrahydrofolate dehydrogenase is expressed by immortal cells
J. Biol. Chem.
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1985
Cricetulus griseus, Cricetus cricetus, Homo sapiens, Mesocricetus auratus, Mus musculus, Rattus norvegicus
brenda
Mejia, N.; Rios-Orlandi, E.M.; Mackenzie, R.E.
NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase from ascites tumor cells. Purification and properties
J. Biol. Chem.
261
9509-9513
1986
Acetobacterium woodii, Clostridium formicaceticum, Mus musculus
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Mackenzie, R.E.; Mejia, N.; Yang, X.M.
Methylenetetrahydrofolate dehydrogenases in normal and transformed mammalian cells
Adv. Enzyme Regul.
27
31-39
1988
Homo sapiens, Mus musculus, Saccharomyces cerevisiae
brenda
Mejia, N.; Mackenzie, R.E.
NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase in transformed cells is a mitochondrial enzyme
Biochem. Biophys. Res. Commun.
155
1-6
1988
Mus musculus
brenda
Rios-Orlandi, E.M.; Mackenzie, R.E.
The activities of the NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase from ascites tumor cells are kinetically independent
J. Biol. Chem.
263
4662-4667
1988
Mus musculus
brenda
Belanger, C.; Mackenzie, R.E.
Isolation and characterization of cDNA clones encoding the murine NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase
J. Biol. Chem.
264
4837-4843
1989
Mus musculus
brenda
Barlowe, C.K.; Appling, D.R.
Isolation and characterization of a novel eukaryotic monofunctional NAD+-dependent 5,10-methylenetetrahydrofolate dehydrogenase
Biochemistry
29
7089-7094
1990
Acetobacterium woodii, Saccharomyces cerevisiae, Clostridium formicaceticum, Homo sapiens, Rattus norvegicus, Saccharomyces cerevisiae KSY8
brenda
Smith, G.K.; Banks, S.D.; Monaco, T.J.; Rigual, R.; Duch, D.S.; Mullin, R.J.; Huber, B.E.
Activity of an NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase in normal tissue, neoplastic cells, and oncogene-transformed cells
Arch. Biochem. Biophys.
283
367-371
1990
Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus, Rattus norvegicus Fischer
brenda
Peri, K.G.; Mackenzie, R.E.
NAD(+)-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase: detection of the mRNA in normal murine tissues and transcriptional regulation of the gene in cell lines
Biochim. Biophys. Acta
1171
281-287
1993
Homo sapiens, Mus musculus, Rattus norvegicus
brenda
Price, B.D.; Laughon, A.
The isolation and characterization of a Drosophila gene encoding a putative NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase
Biochim. Biophys. Acta
1173
94-98
1993
Drosophila melanogaster, Homo sapiens, Mus musculus
brenda
Yang, X.M.; Mackenzie, R.E.
NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene
Biochemistry
32
11118-11123
1993
Homo sapiens, Mus musculus, Saccharomyces cerevisiae
brenda
Tremblay, G.B.; Sohi, S.S.; Retnakaran, A.; Mackenzie, R.E.
NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is targeted to the cytoplasm in insect cell lines
FEBS Lett.
368
177-182
1995
Antheraea eucalypti, Choristoneura fumiferana, Drosophila melanogaster, Homo sapiens, Malacosoma disstria, Mus musculus, Spodoptera frugiperda
brenda
Monzingo, A.F.; West, M.G.; Schelp, E.; Appling, D.R.; Robertus, J.D.
Crystallization of the NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae
Proteins
26
481-482
1996
Acetobacterium woodii, Saccharomyces cerevisiae
brenda
Appling, D.R.; West, M.G.
Monofunctional NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae
Methods Enzymol.
281
178-188
1997
Acetobacterium woodii, Saccharomyces cerevisiae, Clostridium formicaceticum, Drosophila melanogaster, Homo sapiens, Mus musculus
brenda
Mackenzie, R.E.
Mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase
Methods Enzymol.
281
171-177
1997
Saccharomyces cerevisiae, Drosophila melanogaster, Homo sapiens, Mus musculus, Spodoptera frugiperda
brenda
Di Pietro, E.; Sirois, J.; Trenblay, M.L.; Mackenzie, R.E.
Mitochondrial NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is essential for embryonic development
Mol. Cell. Biol.
22
4158-4166
2002
Saccharomyces cerevisiae, Cricetulus griseus, Mus musculus
brenda
Patel, H.; Christensen, K.E.; Mejia, N.; Mackenzie, R.E.
Mammalian mitochondrial methylenetetrahydrofolate dehydrogenase-cyclohydrolase
Arch. Biochem. Biophys.
403
145-148
2002
Drosophila melanogaster, Homo sapiens, Mus musculus
brenda
Takeya, C.; Esumi, M.; Shiroishi, T.; Hishida, R.; Yamamoto, T.
Multiple single-nucleotide polymorphisms in the methylenetetrahydrofolate reductase and its truncated pseudogene of 23 inbred strains of mice
Biochem. Biophys. Res. Commun.
312
480-486
2003
Mus musculus (Q9WU20), Mus musculus
brenda
Di Pietro, E.; Wang, X.L.; MacKenzie, R.E.
The expression of mitochondrial methylenetetrahydrofolate dehydrogenase-cyclohydrolase supports a role in rapid cell growth
Biochim. Biophys. Acta
1674
78-84
2004
Mus musculus (P18155)
brenda
Saffroy, R.; Pham, P.; Chiappini, F.; Gross-Goupil, M.; Castera, L.; Azoulay, D.; Barrier, A.; Samuel, D.; Debuire, B.; Lemoine, A.
The MTHFR 677C > T polymorphism is associated with an increased risk of hepatocellular carcinoma in patients with alcoholic cirrhosis
Carcinogenesis
25
1443-1448
2004
Homo sapiens
brenda
Patel, H.; Pietro, E.D.; MacKenzie, R.E.
Mammalian fibroblasts lacking mitochondrial NAD+-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase are glycine auxotrophs
J. Biol. Chem.
278
19436-19441
2003
Mus musculus
brenda
Patel, H.; Di Pietro, E.; Mejia, N.; MacKenzie, R.E.
NAD- and NADP-dependent mitochondrially targeted methylenetetrahydrofolate dehydrogenase-cyclohydrolases can rescue mthfd2 null fibroblasts
Arch. Biochem. Biophys.
442
133-139
2005
Mus musculus
brenda
Christensen, K.E.; Mirza, I.A.; Berghuis, A.M.; Mackenzie, R.E.
Magnesium and phosphate ions enable NAD binding to methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase
J. Biol. Chem.
280
34316-34323
2005
Homo sapiens (P13995), Homo sapiens
brenda
Buchenau, B.; Thauer, R.K.
Tetrahydrofolate-specific enzymes in Methanosarcina barkeri and growth dependence of this methanogenic archaeon on folic acid or p-aminobenzoic acid
Arch. Microbiol.
182
313-325
2004
Methanosarcina barkeri (Q46A53), Methanosarcina barkeri DSM 804 (Q46A53)
brenda
Shin, M.; Momb, J.; Appling, D.R.
Human mitochondrial MTHFD2 is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
Cancer Metab.
5
11
2017
Homo sapiens (P13995), Homo sapiens (Q9H903)
brenda
Gustafsson, R.; Jemth, A.S.; Gustafsson, N.M.; Faernegardh, K.; Loseva, O.; Wiita, E.; Bonagas, N.; Dahllund, L.; Llona-Minguez, S.; Haeggblad, M.; Henriksson, M.; Andersson, Y.; Homan, E.; Helleday, T.; Stenmark, P.
Crystal structure of the emerging cancer target MTHFD2 in complex with a substrate-based inhibitor
Cancer Res.
77
937-948
2017
Homo sapiens (P13995)
brenda
Hitzel, J.; Lee, E.; Zhang, Y.; Bibli, S.I.; Li, X.; Zukunft, S.; Pflueger, B.; Hu, J.; Schuermann, C.; Vasconez, A.E.; Oo, J.A.; Kratzer, A.; Kumar, S.; Rezende, F.; Josipovic, I.; Thomas, D.; Giral, H.; Schreiber, Y.; Geisslinger, G.; Fork, C.; Yang, X.; Sigala, F.; Romanoski, C.E.; Kroll, J.; Jo, H.; Lan, L.a.n.d.
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
Nat. Commun.
9
2292
2018
Homo sapiens (P13995)
brenda