Information on EC 1.4.99.2 - taurine dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.4.99.2
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RECOMMENDED NAME
GeneOntology No.
taurine dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
taurine + H2O + acceptor = 2-sulfoacetaldehyde + NH3 + reduced acceptor
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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oxidative deamination
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
taurine degradation II
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taurine degradation
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SYSTEMATIC NAME
IUBMB Comments
taurine:acceptor oxidoreductase (deaminating)
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CAS REGISTRY NUMBER
COMMENTARY hide
50812-14-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
quantitative excretion of sulfoacetaldehyde during growth with taurine as sole source of nitrogen
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Manually annotated by BRENDA team
quantitative excretion of sulfoacetaldehyde during growth with taurine as sole source of nitrogen
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Manually annotated by BRENDA team
putative large subunit; growth on N-methyltaurine as sole source of carbon and energy or as sole source of fixed nitrogen for aerobic growth. Secretion of stoichiometric amounts of methylamine
SwissProt
Manually annotated by BRENDA team
putative large subunit; growth on N-methyltaurine as sole source of carbon and energy or as sole source of fixed nitrogen for aerobic growth. Secretion of stoichiometric amounts of methylamine
SwissProt
Manually annotated by BRENDA team
gram-negative
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
upon growth on N-acetyltaurine as sole carbon source, excretion of stoichiometric amounts of sulfate and ammonium. Proposal of degradative pathway
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Manually annotated by BRENDA team
no activity in Castellaniella defragrans
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Manually annotated by BRENDA team
no activity in Castellaniella defragrans NKNTAU / DSM 11046
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Manually annotated by BRENDA team
no activity in Silicibacter pomeroyi
DSS-3
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Manually annotated by BRENDA team
no activity in Silicibacter pomeroyi DSS-3
DSS-3
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Manually annotated by BRENDA team
putative large subunit; growth on N-methyltaurine as sole source of fixed nitrogen for aerobic growth. Secretion of stoichiometric amounts of methylamine
SwissProt
Manually annotated by BRENDA team
putative large subunit; growth on N-methyltaurine as sole source of fixed nitrogen for aerobic growth. Secretion of stoichiometric amounts of methylamine
SwissProt
Manually annotated by BRENDA team
large subunit; partial gene tauY
SwissProt
Manually annotated by BRENDA team
Ralstonia sp. EDS1 / DSM 13640
large subunit
SwissProt
Manually annotated by BRENDA team
Ralstonia sp. SFCD1 / DSM 15091
partial gene tauY
SwissProt
Manually annotated by BRENDA team
exponential growth with taurine as sole source of energy and carbon. Enzyme is inducible by growth on substrate
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Manually annotated by BRENDA team
Rhodobacter sphaeroides ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158
exponential growth with taurine as sole source of energy and carbon. Enzyme is inducible by growth on substrate
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Manually annotated by BRENDA team
Rhodobacter sphaeroides ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158 ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158
exponential growth with taurine as sole source of energy and carbon. Enzyme is inducible by growth on substrate
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Manually annotated by BRENDA team
gene tauXY
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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individual deletion mutants of tauR, tauC, and tauY, show growth defects. Growth is restored when each mutant is complemented with the appropriate gene on a plasmid, suggesting that the individual deletions do not deter expression of downstream genes, they are not polar mutations. Induced or uninduced DELTAtauY mutant displays the highest levels of reporter expression compared to other strains grown under similar conditions. Increased accumulation of taurine, and thus enhanced expression, occurs in the DELTAtauY mutant due to its inability to degrade the sulfonate compound
physiological function
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the genes of the tau region, tauR, tauC, and tauY, are each required for taurine dissimilation in Sinorhizobium meliloti. Enzyme TauY is involved in the degradation of taurine, most likely by forming part of the taurine dehydrogenase
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hypotaurine + H2O + phenazine methosulfate
? + NH3 + reduced phenazine methosulfate
show the reaction diagram
N-methyltaurine + H2O + acceptor
sulfoacetaldehyde + N-methylamine + reduced acceptor
show the reaction diagram
taurine + H2O + 2,6-dichlorophenol indophenol
sulfoacetaldehyde + NH3 + reduced 2,6-dichlorophenol indophenol
show the reaction diagram
taurine + H2O + acceptor
sulfoacetaldehyde + NH3 + reduced acceptor
show the reaction diagram
taurine + H2O + bovine heart cytochrome c
2-sulfoacetaldehyde + NH3 + reduced bovine heart cytochrome c
show the reaction diagram
taurine + H2O + cytochrome c
sulfoacetaldehyde + NH3 + reduced cytochrome c
show the reaction diagram
taurine + H2O + ferricyanide
2-sulfoacetaldehyde + NH3 + ferrocyanide
show the reaction diagram
taurine + H2O + phenazine methosulfate
sulfoacetaldehyde + NH3 + reduced phenazine methosulfate
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
taurine + H2O + acceptor
sulfoacetaldehyde + NH3 + reduced acceptor
show the reaction diagram
taurine + H2O + bovine heart cytochrome c
2-sulfoacetaldehyde + NH3 + reduced bovine heart cytochrome c
show the reaction diagram
taurine + H2O + cytochrome c
sulfoacetaldehyde + NH3 + reduced cytochrome c
show the reaction diagram
taurine + H2O + ferricyanide
2-sulfoacetaldehyde + NH3 + ferrocyanide
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-heptyl-4-hydroxyquinoline N-oxide
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some inhibition
Atebrin
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some inhibition
Isonicotinic acid 2-isopropylhydrazine
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strong inhibition
methylene blue
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some inhibition
Neocuproine
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some inhibition
p-chloromercuribenzoate
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strong inhibition
Phenelzine
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strong inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.056
phenazine methosulfate
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20
taurine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9
optimized assay with beef heart cytochrome c as acceptor
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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40% loss of activity in 20 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Stable to freeze-thawing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 1.5 mg protein per ml, 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
homogenous, precipitation, chromatography steps
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
enzyme might be encoded by genes tauX and tauY, gene cluster DNA and sequence determination and analysis, gene cluster organization
gene tauY (SMb21529) encodes the large subunit of the putative taurine dehydrogenas, expression analysis in wild-type and mutant strainse
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gene tauY, DNA and sequence determination and analysis
genes tauX and tauY, gene cluster DNA and sequence determination and analysis, gene cluster organization
genes tauXY, gene cluster DNA and sequence determination and analysis, gene cluster organization
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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in-frame deletion of gene tauY. Individual deletion mutants of tauR, tauC, and tauY, show growth defects. Growth is restored when each mutant is complemented with the appropriate gene on a plasmid, suggesting that the individual deletions do not deter expression of downstream genes, they are not polar mutations. Induced or uninduced DELTAtauY mutant displays the highest levels of reporter expression compared to other strains grown under similar conditions. Increased accumulation of taurine, and thus enhanced expression, occurs in the DELTAtauY mutant due to its inability to degrade the sulfonate compound