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Information on EC 1.4.5.1 - D-amino acid dehydrogenase (quinone) and Organism(s) Helicobacter pylori and UniProt Accession A3KEZ1

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IUBMB Comments
An iron-sulfur flavoprotein (FAD). The enzyme from the bacterium Helicobacter pylori is highly specific for D-proline, while the enzyme from the bacterium Escherichia coli B is most active with D-alanine, D-phenylalanine and D-methionine. This enzyme may be the same as EC 1.4.99.6.
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This record set is specific for:
Helicobacter pylori
UNIPROT: A3KEZ1
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The taxonomic range for the selected organisms is: Helicobacter pylori
The expected taxonomic range for this enzyme is: Archaea, Bacteria, Eukaryota
Synonyms
DadA, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
D-amino acid:quinone oxidoreductase (deaminating)
An iron-sulfur flavoprotein (FAD). The enzyme from the bacterium Helicobacter pylori is highly specific for D-proline, while the enzyme from the bacterium Escherichia coli B is most active with D-alanine, D-phenylalanine and D-methionine. This enzyme may be the same as EC 1.4.99.6.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Ala + oxidized coenzyme Q1 + H2O
pyruvate + NH3 + reduced coenzyme Q1
show the reaction diagram
specific activity is 37% of that with D-proline
-
-
?
D-Phe + oxidized coenzyme Q1 + H2O
phenylpyruvate + NH3 + reduced coenzyme Q1
show the reaction diagram
specific activity is 20% of that with D-proline
-
-
?
D-proline + oxidized coenzyme Q1 + H2O
DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
show the reaction diagram
-
-
-
?
D-Ser + oxidized coenzyme Q1 + H2O
3-hydroxy-2-oxopropanoate + NH3 + reduced coenzyme Q1
show the reaction diagram
specific activity is 7% of that with D-proline
-
-
?
additional information
?
-
activity with D-Val, D-Met, D-Glu, D-Asp, L-Pro and L-Ala is below 5% of the activity with D-Pro
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Mg2+ and Ca2+ have no effect on DadA activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
benzoate
0.1 mM, 98% inhibition
iodoacetamide
0.1 mM, 66% inhibition
iodoacetic acid
0.1 mM, 62% inhibition
p-hydroxybenzoic acid
0.1 mM, 79% inhibition
additional information
EDTA, Mg2+ and Ca2+ have no effect on DadA activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40.2
D-proline
pH 8.0, 37°C
0.0082
oxidized coenzyme Q1
pH 8.0, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
pH 7.5: about 50% of maximal activity, pH 8.5: about 45% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 55
25°C: about 50% of maximal activity, 55°C: about 65% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NCTC 11637
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DADA_HELPX
410
0
46041
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44000
x * 44000, SDS-PAGE
46040
x * 46040, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from Helicobacter pylori and recombinant Escherichia coli cells
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tanigawa, M.; Shinohara, T.; Saito, M.; Nishimura, K.; Hasegawa, Y.; Wakabayashi, S.; Ishizuka, M.; Nagata, Y.D
D-Amino acid dehydrogenase from Helicobacter pylori NCTC 11637
Amino Acids
38
247-255
2010
Helicobacter pylori (A3KEZ1)
Manually annotated by BRENDA team