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Information on EC 1.4.3.22 - diamine oxidase and Organism(s) Rattus norvegicus and UniProt Accession P36633

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EC Tree
     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.22 diamine oxidase
IUBMB Comments
A group of enzymes that oxidize diamines, such as histamine, and also some primary monoamines but have little or no activity towards secondary and tertiary amines. They are copper quinoproteins (2,4,5-trihydroxyphenylalanine quinone) and, like EC 1.4.3.21 (primary-amine oxidase) but unlike EC 1.4.3.4 (monoamine oxidase), they are sensitive to inhibition by carbonyl-group reagents, such as semicarbazide.
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Rattus norvegicus
UNIPROT: P36633
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The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
diamine oxidase, amine oxidase, histaminase, histamine dehydrogenase, histamine oxidase, amiloride-binding protein, dao-1, n-methylputrescine oxidase, rhdao, methylputrescine oxidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Diamine oxidase
-
SYSTEMATIC NAME
IUBMB Comments
histamine:oxygen oxidoreductase (deaminating)
A group of enzymes that oxidize diamines, such as histamine, and also some primary monoamines but have little or no activity towards secondary and tertiary amines. They are copper quinoproteins (2,4,5-trihydroxyphenylalanine quinone) and, like EC 1.4.3.21 (primary-amine oxidase) but unlike EC 1.4.3.4 (monoamine oxidase), they are sensitive to inhibition by carbonyl-group reagents, such as semicarbazide.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzylamine + O2 + H2O
benzaldehyde + NH3 + H2O2
show the reaction diagram
-
-
-
?
beta-phenylethylamine + O2 + H2O
beta-phenylethanal + NH3 + H2O2
show the reaction diagram
-
-
-
?
histamine + O2 + H2O
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
show the reaction diagram
DAO
-
-
?
tyramine + O2 + H2O
1H-indol-3-ylacetaldehyde + NH3 + H2O2
show the reaction diagram
-
-
-
?
benzylamine + H2O + O2
benzaldehyde + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
histamine + H2O + O2
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
show the reaction diagram
-
preferred substrate
-
-
?
phenylethylamine + H2O + O2
?
show the reaction diagram
-
-
-
-
?
RCH2NH2 + H2O + O2
RCHO + NH3 + H2O2
show the reaction diagram
-
-
-
?
tyramine + H2O + O2
(4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
histamine + H2O + O2
(imidazol-4-yl)acetaldehyde + NH3 + H2O2
show the reaction diagram
-
preferred substrate
-
-
?
RCH2NH2 + H2O + O2
RCHO + NH3 + H2O2
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cu2+
-
Cu2+ ions stimulate the activation/maturation of the enzyme. The highest activity is observed at more than 5fold molar ratio between Cu2+ and the enzyme
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Semicarbazide
very strong inhibition
aminoguanidine
-
-
histamine
-
substrate inhibition
additional information
clorgyline and deprenyl do not decrease DAO activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.64
benzylamine
-
in 100 mM potassium phosphate, pH 7.2, at 37°C
0.035
histamine
-
in 100 mM potassium phosphate, pH 7.2, at 37°C
0.45
tyramine
-
in 100 mM potassium phosphate, pH 7.2, at 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30
histamine
-
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00017
substrate histamine, at low substrate concentration
0.00037
substrate beta-phenylethylamine, at high substrate concentration
0.00074
substrate tyramine, at high substrate concentration
0.00075
substrate benzylamine, at high substrate concentration
0.00226
substrate histamine, at high substrate concentration
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
nine-week-old male Wistar rats
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
amitriptyline at 0.03, 0.05, 0.1 and 0.5 mM concentrations lower enzyme activity in the plasma of the heparinised rat by 14, 22, 23 and 28%, respectively
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
mainly in cytoplasm but to a considerable extent also in mitochondrial fraction
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AOC1_RAT
746
0
85021
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
-
x * 80000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 80000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex 200 gel filtration
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in COS-1 cells
expressed in Escherichia coli Origami2(DE3) cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bieganski, T.; Kusche, J.; Lorenz, W.; Hesterberg, R.; Stahlknecht, C.D.; Feussner, K.D.
Distribution and properties of human intestinal diamine oxidase and its relevance for the histamine catabolism
Biochim. Biophys. Acta
756
196-203
1983
Canis lupus familiaris, Cavia porcellus, Oryctolagus cuniculus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa
Manually annotated by BRENDA team
Ochiai, Y.; Itoh, K.; Sakurai, E.; Adachi, M.; Tanaka, Y.
Substrate selectivity of monoamine oxidase A, monoamine oxidase B, diamine oxidase, and semicarbazide-sensitive amine oxidase in COS-1 expression systems
Biol. Pharm. Bull.
29
2362-2366
2006
Rattus norvegicus (P36633)
Manually annotated by BRENDA team
Rajtar, S.; Irman-Florjanc, T.
Amitriptyline affects histamine-N-methyltransferase and diamine oxidase activity in rats and guinea pigs
Eur. J. Pharmacol.
574
201-208
2007
Cavia porcellus, Rattus norvegicus
Manually annotated by BRENDA team
Rosini, E.; Nossa, S.; Valentino, M.; DArrigo, P.; Marinesco, S.; Pollegioni, L.
Expression of rat diamine oxidase in Escherichia coli
J. Mol. Catal. B
82
115-120
2012
Rattus norvegicus
-
Manually annotated by BRENDA team