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Information on EC 1.4.3.2 - L-amino-acid oxidase and Organism(s) Bothrops pauloensis and UniProt Accession B5AR80

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     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase
IUBMB Comments
A flavoprotein (FAD).
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This record set is specific for:
Bothrops pauloensis
UNIPROT: B5AR80
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The taxonomic range for the selected organisms is: Bothrops pauloensis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
laao, il4i1, l-amino-acid oxidase, l-aao, escapin, head kidney and gill, dolabellanin, l-phenylalanine oxidase, akbu-laao, m-lao, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aromatic L-amino acid oxidase
-
-
-
-
L-amino acid oxidase
-
-
-
-
L-amino acid:O2 oxidoreductase
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-
-
-
L-aminooxidase
-
-
-
-
LAO
-
-
-
-
ophio-amino-acid oxidase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
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oxidative deamination
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-amino-acid:oxygen oxidoreductase (deaminating)
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
9000-89-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-Ala + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Arg + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Glu + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
best substrate
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Trp + H2O + O2
indol-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Val + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Ile + H2O + O2
3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
LAAO shows leishmanicidal, antitumoral and bactericidal activities dose dependently, overview. LAAO induces platelet aggregation in platelet-rich plasma which is inhibited by catalase
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
a flavoenzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110
purified enzyme, substrate L-Val
140
purified enzyme, substrates L-Tyr or L-Glu
310
purified enzyme, substrate L-Trp
335
purified enzyme, substrate L-His
340
purified enzyme, substrate L-Arg
480
purified enzyme, substrate L-Ile
545
purified enzyme, substrate L-Phe
575
purified enzyme, substrate L-Leu
580
purified enzyme, substrate L-Met
60
purified enzyme, substrate L-Ala
additional information
substrate specificity, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.3
sequence calculation
6.3
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme exhibits cytotoxic, bactericidal, leishmanicidal, and antiviral effects
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
OXLA_BOTPA
503
0
56799
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation
65000
2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 65000, SDS-PAGE, 2 * 58000, about, sequence calculation
homodimer
-
2 * 65000, SDS-PAGE
additional information
structure analysis and molecular dynamics simulation, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from venom by cation exchange and hydrophobic interaction chromatography, followed by adosrption chromatography and HPLC
CM-Sepharose column chromatography, phenyl-Sepharose CL-4B column chromatography, benzamidine Sepharose and C18 RP column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rodrigues, R.S.; da Silva, J.F.; Boldrini Franca, J.; Fonseca, F.P.; Otaviano, A.R.; Henrique Silva, F.; Hamaguchi, A.; Magro, A.J.; Braz, A.S.; dos Santos, J.I.; Homsi-Brandeburgo, M.I.; Fontes, M.R.; Fuly, A.L.; Soares, A.M.; Rodrigues, V.M.
Structural and functional properties of Bp-LAAO, a new L-amino acid oxidase isolated from Bothrops pauloensis snake venom
Biochimie
91
490-501
2009
Bothrops pauloensis (B5AR80)
Manually annotated by BRENDA team
Izidoro, L.F.; Sobrinho, J.C.; Mendes, M.M.; Costa, T.R.; Grabner, A.N.; Rodrigues, V.M.; da Silva, S.L.; Zanchi, F.B.; Zuliani, J.P.; Fernandes, C.F.; Calderon, L.A.; Stabeli, R.G.; Soares, A.M.
Snake venom L-amino acid oxidases trends in pharmacology and biochemistry
BioMed Res. Int.
2014
196754
2014
Deinagkistrodon acutus, Calloselasma rhodostoma, Bothrops atrox, Bothrops jararaca, Bothrops marajoensis, Bungarus fasciatus, Lachesis muta, Naja oxiana, Ophiophagus hannah, Daboia russelii, Macrovipera lebetina, Bothrops alternatus, Bothrops pirajai, Gloydius ussuriensis, Bungarus caeruleus, Aplysia punctata, Bothrops insularis, Bothrops pauloensis, Crotalus durissus cumanensis, Bothrops leucurus, Vipera berus berus
Manually annotated by BRENDA team